Annals of Mechnikov's Institute 2008 1
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Contents)



C. (P.)


(Editorial Board)

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(Contents)

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(REVIEWS)





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ULTRASOUND USE FOR MICROBIAL CELLS DECOMPOSITION
Isaenko E. YU.
The analysis of the data of the literature about ultrasound used in biology, medicine and industry is presentedin the article.  The  aspects  of  the  sound  factor  influence  on bacteria is chosen. The application of the low and medium frequency acoustic waves for microbial cell decomposition with  the  purpose  of  getting  of  the  different  antigen complexes  for  vaccine  preparation  elaboration  is described.
Key  words:  ultrasound,  antigens,  decomposition, microbial cell, vaccines.


5-9

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(EXPERIMENTAL STUDY)



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CLINIC AND EPIDEMIOLOGY OF THE SMALL FORM INFILTRATIVE PULMONARY TUBERCULOSIS IN CHILDREN AND MODERN POSSIBILITIES OF DETERMINATION OF SPECIFIC ACTIVITY
Kostromina V. P., Strizh V. ., Belogortseva O. I., Symonenkova N. V., Sivachenko . Ye, Rechkina . ., Yaroschuk L. B.
The  features  of  the  limited  form  of  ITL  are  studied by randomized controlled  clinical research among 12 children 13 14 years old (group) and 43 persons 15 17 years old  ( group).   Tuberculosis  in  children  and  teenagers today  appears  mainly  at  an  appeal  for  medical  help, at different intensity of reactions for a sample Mantoux, and in 7,2 p.s. of a negative cases test. On the initial stages of  disease 53,9 p.s. patients had disorganization of backlogs of adaptation toward an overactivation or stress (4:1), that it is necessary to take into account during the reception of  prophylactic  medical  care.  For  the  aims  of  differential diagnostics of infiltrate changes in lights it is expedient to apply  the  photohystochemical  research  of  peripheral blood.


10-14

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ROLE  OF  MOTHER'S  MILK  AS  A  FACTOR  OF TRANSMISSION  OF  HERPESVIRUS   INFECTION FROM  AN  INFECTED  MOTHER  TO  THE NEWBORN
Panchenko L.A., Torianyk I.I., Popova N.G.
This article presents information  which proves pathogenic way  of  HSV-infection  transmission  from  seropositive puerperants to their newborns with diagnosed syndrome of intrauterine  growth  retardation.  Authors  discuss  clinical and  laboratory,  morphological  arguments  which  prove possible  participation  of  breast  milk  cell  pool  in transportation and reproduction of HSV causative agent.
Key  words:  Mothers  milk,  HSV-infection,  factor  of transmission, cell pool, puerperants.

15-17

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INFECTIONS AT THE INFLAMMATORY DISEASES OF WOMANISH PRIVY PARTS
Kvitka N.V. , Peremot S.D. , Smelyanskaya M.V.
We  conducted  determination  of  exciters,  transmissible sexual a way at such widespread gynecological diseases as salpingities,  oofarities  and  erosion  of  neck  of  uterus.  293 patients,  being  on  treatment  in  Center  heals  of  woman  of the South railway, are inspected. Determination of  markers of  exciters  of  disease  was  conducted  through  an  ELISA analysis and MFA method. Findings testify to the high level of  infected  of  patients,  especially,  in  repressing  majority cases  took  a  place  associated  bacterial-viral  infection. Pathological  process,  caused  mixt-infection  characterized more expressing conducted treatments a clinical picture and less efficiency.
Keywords: mixt infections, salpingitis, oofaritis, erosiaof  uterus, reproduction health.

18-20

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EXPERIMENTAL STUDY OF BIOLOGICAL MATERIAL CYTOPATHOGENIC FOR CELL CULTURE McCoy AND L 929
Mavrov I.I., Kondakova A.K., Dzhoraeva S.K., Goncharenko V.V., Kutova V.V.
It was described the biological material cytopathogenic determination for cell culture McCoy and L 929. The sensitiveness cell culture McCoy and L 929 is compared with inoculation of joint juice and infected yolk sac in the received results. The necessary subsequent culturalstudy for optimal inoculation conditions of the agent from the biological material is confirmed by the data.
Key words:the cell culture McCoy and L 929, the cultivation, the joint juice, infected yolk sac.

21-26

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CLINICAL AND MICROBIOLOGIC PARALLELS OF COURSES AND RESULTS OF UROGENITAL INFLAMMATORY PROCESSES OF BACTERIAL AND VIRUS ETIOLOGY IN WOMEN
Voropay A.Ur.
Chlamydial  and  herpes  infection  problem  is  very  actual nowadays.  Urogenital  chlamydiosis  is  often  accompanied by  herpes  simplex  infection  in  clinical  observation. Chlamydial  and  herpes  mix  infection  makes  the  agents more  adopted  to  the  intracellular  persistence  and  enhance the pathogenic abilities and resistance each of theagents to the  influence  of  the  antibacterial  substances.  This is  very good  observed  in  chronic  cystitis,  as  an  example,  that accrue in women with chlamydial herpes infection.Alas, the  questions  of  diagnostic  and  treatment  measures  and specification of curse of disease are not solved properly.
Key  words:  herpes  simplex  infection  Urogenital chlamydiosis diagnostic and treatment
 

27-29

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ANALYSIS OF ANTIBIOTICRESISTENCE OF PATHOGENIC AND ESSE-PATHOGENIC MICROORGANISMS, WITHDRAWN FROM CHILDREN, PATIENTS WITH A ACUTE INTESTINAL INFECTION
Voronkina I.A., Derkach S.A., Kotsar O.V., Nosatenko A.I., Krilova I.A., Piddubna N.A., Gabisheva L.S.
In  modern  terms  the  problem  of  resistance  of microorganisms  acquired  global  value.  Be  the  method of diffusion  in  agar  with  the  use  of  disks  was  certainsensitiveness  of  162  cultures  of  shigels,  237  cultures  of salmonels  and  161  culture  esse-pathogenic microorganisms  to  aminoglikozids,  phtorhinolons, cefalosporins, levomicetin, doxicilins and ampicillin. Most the  indexes  of  sensitiveness  were  aminoglikozids  on the second  place.  Most  level  resistance  is  discovered  in relation to levomicetin, doxicilin and ampicillin. Universal to  the  antibiotic  to  which  a  high  sensitiveness  (>90%) would  be  sufficiently  both  pathogenic  enterobacteria  and cultures of EPM, in this research not it is found.
Keywords:  sharp  intestinal  infections,  salmonella, shigella,  esse-pathogenic microorganisms, antibioticresistance.


30-37

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SYNTHESIS,PROPERTIES AND STABILITY TO AN AMYLASE OF Β-CYCLODEXTRIN ACYL DERIVATIVES
Lisnyak Yu.V., Martynov A.V., Klemchuk L.V.
Synthesis  of  β-cyclodextrin  acyl  derivatives  (succinylated, maleylated and aconitylated) was carried out, theirmolecular  masses  and  charges  were deter-mined,  stability  to  an amylase action was studied. It was determined that reaction of aconitylation is not terminated completely: three alcohol hydroxyls in the struc-ture of cyclodextrin remain unsubstituted.  Besides,  just  aconitylated derivative  of  pcyclodextrin remains unstable to an amylase action  and is destroying  like  non-acylated  β-cyclodextrin.  Succinylated and  maleylated  derivatives  become  stable  to  an  amylase action,  their  molecular  masses  and  charges  coincide with calculated ones.
Key  words: synthesis,  β-cyclodextrin,  amylase,  aconitylated, succinylated

38-41

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̲ Ͳ Ҳ ʲ CETRARIA ISLANDICA(L.) ACH. EVERNIA PRUNASTRI(L.) ACH. ² Բʲ в в Ҳ˲Ҳ
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NTIMICROBIAL ACTIVITY OF LICHENS CETRARIA ISLANDICA (L.) Ach . AND  EVERNIA PRUNASTRI (L.) Ach. EXTRACTS AGAINST STAPHYLOCOCCI OF VARIOUS METHICILLIN RESISTANCE LEVEL
Kutsyk R.V.
The  significant  bactericidal  activity  of  lichens  iceland  moss Cetraria islandica (L.) Ach. and  oak  moss  Evernia prunastri (L.)  Ach.  thallus  hexane  extracts  against  clinical  strains  of staphylococci  including  methicillin-resistant  isolates  by  agar diffusion and agar dilution technique was established.

42-46

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WIDE EPIDEMIOLOGICAL EDUCATIONAL OF UKRAINE PEOPLE - IMPORTANT FACTOR OF BIOSAFETY STRENGTHENING
Neporada V.P., Kononenko V.V., Volianskiy A.J., Ignatyev O.M., Yrmula K.A., Savitskiy J.V., Neporada V.V.,Matsegora N.A.
The  problem  of  infectious  threat  of  a  civilization is recognized  for  today  in  the  world  priority  alongside  with economic, power safety and the international terrorism. Owing to natural laws and anthropogenic activity activation of many known  and  occurrence  of  new  infectious  diseases  is  now observed.  Therefore  epidemiological  formation  of  the population has the important state value for achievement of a necessary  level  of  biosafety  of  our  country.  Such epidemiological  educational  program  should  become obligatory  for  workers  of  the  Ministry  of  Emergency Measures, power Ministries,  municipal  institutions  all levels, the enterprises of transport, tourism, trade, pupils of schools, students and other categories of citizens. Basic knowledge in the field of epidemiology should be transformed at  personal level in epidemiologically correct behavior and at society level should be promote to localizations and liquidation  of possible flashes of infectious diseases.
Keywords: infectious diseases, biosafety, epidemiological
education
47-49

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