Annals of Mechnikov's Institute 2007 2
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Contents)



C. (P.)


(Editorial Board)

1

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(Contents)

2-5

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(REVIEWS)




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VANCOMYCIN RESISTANT ENTEROCOCCUS (THE REVIEW OF PAPERS)
Myronenko L. G., Peretyatko E. G.
Papers data about the role of Vancomycin-resistant enterococcus in appearing of nosocomial infection are generalized. The mechanisms of resistance formation to glycopeptidal antibiotics are described. The necessity of microbiological monitoring of vancomycin resistance of enterococcus conducting is grounded.
Key words: enterococcus, vancomycin resistance, microbiological monitoring.

6-10

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ֲ RAHNELLA AQUATILIS: ˲ в Ҳ, ʲ ²² Ͳ Ͳ Ͳ Ͳ
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PATHOGENIC POTENTIAL OF RAHNELLA AQUATILIS: THE STUDY OF PATHOGENS FACTORSBEING RESPONSIBLE FOR RAHNELLA ABILITY TO COLONIZE SUSCEPTIBLE TISSUES OF HOST ORGANISM
Pokhil S.I.
In 24 strains of R. aquatilis, there were revealed pathogens factors which provide these microorganisms with potential to colonize susceptible tissues of host organism: high adhesive activity; ability to produce extracellular metabolites having bacteriocinic and repellent activity to representatives of indigenic microflora of human intestine; antilysozyme activity. R. aquatilis strains showed low level of proteinase and neuroaminidase activity and did not produce lecithinase. Termoregulation effects on intensity action of R. aquatilis pathogens factors.
Key words: Rahnella aquatilis, pathogens factors, colonization of susceptible tissues.

11-18

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(EXPERIMENTAL STUDY)



Բֲ ̲ YERSINIA SPP. Ҳ
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IDENTIFICATION STRAINS OF YERSINIA SPP. AND TESTING OF RESISTANCE

Vyalykh Zh.E., Sutulova A.A.,. KilipkoL.V.

The species identification of freshly isolated strains was performed with the help of biochemical testing (the total number of strains was 68) also the sensitivity of this strains to antibiotics was researched. 95,2% of strains which were researched were resistant to ampicilline, 75,8 % to chloramphenicol, 59,7 % to tetracycline, 98,4 100,0% of them were sensitive to ciprofloxacine, cephalosporins, aminoglicozids, imipenem.
Key words: Yersinia, biochemical propertie, resistance.

19-21

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STUDY OF THE MICROBIAL COMPOSITIONS SPECIALITIES OF THE WOUNDED EXUDATE AFTER OF LAPAROSCOPIC AND LAPAROTOMIC OPERATIONS IN BILIARIC TRACTS
Yagniuk Yu.A., Mariushchenko A.V., Bacumenko A.V., Volkov A.A., Piatacov A.V.
Microbial compositions of the wounded exudate, which from patients, was studied, after laparoscopy and laparotomy surgical interferences in biliaric tracts. It was discowered, that, in comparison with laparotomic operations, after laparoscopic cholecystectomy in the wounded exudate during the first day after operacions the maintenance of E.coli and asporogenic anaerobic bacteries, represented Bacteroides spp., was increased.
Key words: laparoscopic and laparotomic cholecystectomy, postoperative period, bacterial compositions

22-24

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C.
DIPHTHERIAE

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DISCOVERY OF GENETIC POLYMORPHISM OF C. DIPHTHERIAE MUSEUM CULTURES IN POLYMERASE CHAIN REACTION WITH ARBITRARY PRIMERS
Myronenko L.G., Sauchuk A.I.
The 12 C. diphtheriae museum cultures were investigated by polymerase chain reaction with arbitrary primer 45. These cultures were isolated in 1954-1959 in Moscow and in 1991 in Kharkov from the patients with diphtheria and the bacilli carriers. The most typical amplicon profiles the museum cultures were determined. The level of genetic similarity of the museum cultures, which manifests by coincidence of the amplicon profiles, was determined too. This information will allow supplement the certificates of the museum cultures and creation of the data bank, which contains information about of the pathogene genomes circulating in various regions and different epidemic process intensity.
Key words: C. diphtheriae, polymerase chain reaction with arbitrary primers, certificate of bacterial culture.

25-28

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Ͳ Ҳ-Ҳ ′ ̲ Ͳ IN VITRO
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SCREENING OF MICROBIAL ORIGIN PREPARATIONS WITH POTENTIAL ADJUVANT PROPERTIES ACCORDING TO THEIR INFLUENCE UPON PHAGOCYTIC ACTIVITY IN VITRO
Yelyseyeva I.V., Babych Ye.M., Sklyar N.I., Volyansky Yu.L., Belozersky V.I., Zhdamarova L.A., Myshukova S.A., Sydorenko T.A., Igumnova N.I., N.I.Yevsyukova V.Yu., Ryzhkova T.A.
Undertaken study is devoted to search of effective microbial origin adjuvants for development immunogenic oral vaccines against diphtheria. Its investigated influence of filtrates of microbial cultures prepared from strains of Corynebacterium and Lactobacillus spp. upon phagocytic activity of humans and animal neutrophilic leukocytes in vitro. Filtrates prepared from C. pseudodiphtheriticum and L. plantarum appeared as the most perspective ones according to investigated indices of phagocytosis.
Key worlds: adjuvants, phagocytosis, vaccines against diphtheria, Corynebacterium, Lactobacillus.


29-33

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Ͳ ̲ - ̲ ² в в ² Ͳֲ -
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SUPEROXIDE DISMUTASE ACTIVITY AND ENZYMATICALLY ACTIVE CERULOPLASMIN CONTENT IN BLOOD SERUM OF DIFFERENT AGE RATS IN CONDITIONS OF ADT ANATOXIN IMMUNIZATION
Volyanskiy A.Y., Nikitchenko Yu.V., Simirenko L.L., Kuchma I.Y.
Superoxide dismutase activity and enzymatically active ceruloplasmin content in blood serum of 1-, 3-, 12-, 24- and 32month old control male Wistar rats, and dynamics of change of these parameters in 3 and 20 month old rats during the forming of antitoxic immunity to diphtheria and tetanus anatoxins under immunization by ADT vaccine were investigated. The activity of enzymes utilizing superoxide radicals characterizes by age peculiarities both before immunization and in dynamics of immune response to vaccination. Key words: superoxide dismutase, ceruloplasmin, ADT anatoxin, rats, age.


34-37

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