Annals of Mechnikov's Institute 2009 2
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Contents)



C. (P.)


(Editorial Board)

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(Contents)

2-4

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(REVIEWS)



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EHRLICHIOSIS IS A NEW PROBLEM FOR MEDICINE
Kilipko L.V., Timchenko O.M., Pokhil S.I., Yurko K.V.
Ehrlichia infection, ehrlichiosis, is a group of transmissive human and mammal diseases caused by intracellular pathogens, bacteria of genera Ehrlichia. There have been analyzed scientific achievements and considered generalizations concerned classification and biological properties of bacteria of genera Ehrlichia (including sensitivity to antibiotics and pathogen potential), pathogenesis, epidemiology, clinical presentation, up-todate methods of laboratory diagnostics, treatment and prophylaxis of ehrlichiosis.
Key words: ehrlichiosis, properties of causative agent, pathogenesis, epidemiology, clinics, diagnostics, treatment, prophylaxis.


5-13

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THE APPLICATION OF POLYMERASE CHAIN REACTION IN YERSINIA INFECTIONS DIAGNOSTICS
Polishchuk N.N., Misin V.V., Novikov S.V., Shatilo Y.V., Kuchma I.Y.
The modern sight at the possibilities of polymerase chain reaction application in Yesinia infections diagnostics is presented in the work. The data about high effectiveness of PCR, as compared with conventional methods of research, in revealing of intestinal yersiniosis and pseudotuberculosis are represented. The results of genetic structure analysis of Y.pseudotuberculosis and Y.enterocolitica, that are necessary for working out the epidemiological markers for Yesinia distribution surveillance are described. The data from references testify to necessity of development of new and improvement of existing genemolecular research methods for Yesinia infections diagnostics.
Key words: Y.pseudotuberculosis, Y.enterocolitica, PCRresearches, a genotype, genotyping, epidemiological surveillance


14-18

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THE VAGINAL MIKROFLORA OF REPRODACTIVE AGE`S WOMEN IN THE NORM AND AT DIFFERENT PATOLOGIC PROCESSES. REVIEW.
Volkov A.O., Bolshakova G.M.
The data of scientific literature, concerning the microflora of wounds of various origin are presented. Modern approaches of the treatment with use of various physical and chemical methods of influence on the microorganisms and their sensitivity to antibiotics are considered.
Key words: microflora of wounds, treatment, antibiotics, antiseptics.

19-23

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(EXPERIMENTAL STUDY)



IMMUNOLOGICAL FEATURES OF THE MODEL OF RADIATION- INDUCED ANTI- INFECTIOUS RESISTANCE DEFECT
Kolyada T.I., Brusnik S.V., Sklyar N.I., Michailichenko M.S.,  Nesterenko A.M., Kolyada O.N.

In course of the present work a model of radiationinduced anti-infectious resistance defect was developed. The research has shown that infection with Salmonella typhimurium of the postnatally irradiated Wistar rats in the dose range of 0,75 - 1,5 Gy leads to the complex defect of anti- infectious resistance, characterized with the following features of immune system activation: phagocytosis depression, the increase in proinflammatory cytokines levels -TNF-α level was (94,072,67) pg/ml, the decrease in the general complement level (36,751,75) h.u. and an increase in circulating immune complexes (0,0090,0007) o.d.u. The developed model can be used in order to evaluate the consequences of irradiation at any time after exposition and methods of their correction.
Key words: low-dose irradiation, anti-infectious resistance, Salmonella typhimurium, immunological parameters.

24-28

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CLINICO-MORPHOLOGICAL ANALYSIS OF THE MATERIALS FROM
ENT-PATIENTS WITH MIXED VIRAL-BACTERIAL INFECTION
Torianyk I.I., Panchenko L.A., Popova N.G., Korovaeva I.V.
The article contains the materials on clinicomorphological diagnosis in ENT- patients with mixed viral and bacterial infection. It is specified that the conditions of mixed infection development increase the etiopathogenic effects which makes the disease clinical presentation significantly more severe, its manifestations polymorphous and prolongs its clinical course. The somatic signs typical for infection diseases are supplemented by mental disorders, which show the inversion of personal and volitional properties. The formation of the symptom complex effects the organism in a whole against a background of structural and functional changes of its organs (pharynx, larynx, bronchi) which can have both specific and unspecific character. It is interesting that dominating are the signs of morphologic changes, which have been traditionally treated as virogenic (nucleus- to- cytoplasm ratio, nonstable sorption ability of cytoplasm, sorption and cumulation ability of nuclei).
Key words: ENT- patients, clinico-morphological analysis, mixed viral and bacterial infection, pharynx, larynx, bron

29-31

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EFFECTS OF ELECTRON BEAM AND OZONE COMBINED PROCESSING ON VARIABILITY OF THE RESIDUAL MICROFLORA
Babych E.M., Sklyar N.I., Ryzhkova T.A., Kalinichenko S.V., Semerenskaya E.I.
The article is devoted to study of possible phenotypic changes in individual representatives of the sanitarymodel microflora in natural water and sewages induced by the electron beam and ozone combined processing. The results obtained at the given stage permit us to state that the presented technique of the water disinfection does not injure microecology of the environment.
Key words: variability of microorganisms, disinfection of water, ozone, electron beam, sanitary-indicative microorganisms


32-36

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THE INFLUECE OF ENHANCERS ON APPEARING RATE OF BACTERIALS AND FUNGOUS COLONIES
Osolodchenko T.P., Batrak E. A., Mizin V.V., Malanchuk S.G., Chuprinova S.I., Zavada N. P., Rjabova I.S., Shtiker L.G.
Influence of growth factors of microorganisms (enhancers) on occurrence of the first colonies and on increase in a biomass of microbic cells and fungous is studied. It is investigated,that increase qualities of microorganisms considerably increase on nutrient mediums with addition enhancers in comparison with standards medium. Concentration enhancers slightly Influences speed of increase in a form make colony of microorganisms and mushrooms for the certain interval of time.
Key words: microorganisms, fungous, medium, enhancers

37-40

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CREATION APATITE-ANTIBACTERIAL OF A COMPOSITE COVERING ON TITANIC SUBSTRATE WITH USE OF THERMODEPOSITARY TECHNOLOGY
Sukhodub L.B., Volyanskiy A.Yu., Osolodchenko T.P., Kuchma I.Yu., Volkov A.A., Mizin V.V.
Apatite-antimicrobial composite coverings on the titanic substrate obtaind by thermodepositary technology have been studied. Antimicrobic activity of 4 titanic plates against referential strains of Staphylococcus, Escherichia, Pseudomonas and Candida has been studied. It has been proved, that aethonium and decamethoxinum in enough quantity is sorbed on a surface of a titanic composite that attacas to structure prolonged antimicrobic activity.
Key words: apatite, the titan, aethonium, decamethoxinum, referential microbes, activity.

41-49

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ANTIBIOTICRESISTANCE OF THE PURULOINFLAMMATORY COMPLICATIONS` AGENTS IN THE ABDOMINAL SURGERY
Diachenco V.F. Mariushchenko A.M., Yagniuk Yu.A., Isaeva S.J. Volkov A.A., Bacumenko A.V., Yagniuk A.I., Piatacov A.V.
Species spectrum of the puruloinflammatory complications` agents was founded by bacteriological research, resistance level of the identifid aerobic and anaerobic agents species to the basic groups of modern antibacterial preparations was studied.

50-52

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HETEROGENEITY BY CRITERIA OF CULTURAL PROPERTIES AND BIOCHEMICAL ACTIVITY OF MICROBES OF GENUS STAPHYLOCOCCUS RECEIVED FROM SICK BY PNEUMONIA CHILDREN A DURING THE PERIOD WITH 2003 FOR 2008
Shatilo Y.V.
The characteristic of biological properties of bacteria of genus Staphylococcus picked out from sick by pneumonia children is resulted. Heterogeneity of isolates of a staphilococcus by criteria of cultural properties and biochemical activity is noted.
Key words: Staphylococcus, cultural properties, biochemical activity, pneumonia

53-57

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ANTIGENIC AND ADJUVANT PROPERTIES OF EXPERIMENTAL SAMPLES OF PREPARATIONS OF SUBCELLULAR COMPLEXES OF THE ACTIVATOR OF THE DIPHTHERIA AT IMMUNIZATION OF LABORATORY ANIMALS
Yelyseyeva I.V., Babych Ye.M., Zhdamarova L.A., Belozersky V.I., Sklyar N.I., Kolpak S.A., Bobireva I.V.
The carried out researches are devoted working out of approaches to improvement of vaccinal preparations against a diphtheria by reception of antigene microbic fractions of the activator which would raise antibacterial immunity as the factor of localisation of a diphtheritic infection in an organism and would stimulate development of antitoxic immunity. Were studied antigene and adjuvant properties of experimental samples of antigenic preparations of the activator of a diphtheria. The received antigene fractions of subcellular complexes of a diphtheria are capable to stimulate antitoxic humoral immunity at introduction together with cleared concentrated diphtheritic toxoid and independently using parenteral and oral immunization schemes.
Key words: antigenic and adjuvant properties, preparations,
diphtheria, immunization

58-65

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GLUCOCORTICOID PART OF ANTIBODY GENESIS REGULATION AT THE HYPOTHYROID STATE OF THE ORGANISM IN EXPERIMENT
Kuchma I.J.

A glucocorticoid part of antibody genesis regulation in dynamics of formation of the humoral immune answer to diphtheritic and tetanic anatoxins of ADS-vaccine at euthyroid and hypothyroid 3-month's Wistar males rats was investigated. Changes of corticosteron serum concentration in the course of the immune answer formation at the background of an organism hypothyroid state, induced by 1-1- 2-mercaptoimidazol introduction, was described by empirical function of regression. Serum level of glucocorticoid hormones before vaccination and in an inductive phase of humoral immune answer formation were bound to specific antibody genesis activity, that are illustrated by charts of dispersion and mathematically described by models of correlation and empirical functions of regression.
Keywords: an organism hypothyroid state, antibody genesis, corticosteron, ADT-anatoxin, rats.


66-72

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