Annals of Mechnikov's Institute 2011 2
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Contents)



C. (P.)


(Editorial Board)

1

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(Contents)

2-4

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(EXPERIMENTAL STUDY)



DETERMINATION OF REGIMES FOR DIPHTHERIA EXOTOXIN MODIFICATION BY  CHEMICAL AND PHYSICOCHEMICAL METHODS
Babych E.M., Kalinichenko S.V., Ryzhkova T.A., Sklyar N.I., Ryabovol O.V., Pluhator T.M., Antusheva T.I., Panova C.V.
The possibility of diphtheria toxoid obtaining using chemical (amino sugars, organic acids) and physicochemical (amino sugars, organic acids, ultrasound, temperature) factors was studied. It was established that modifiers (including formaldehyde) volume content decreasing didnt have significant influence on diphtheria toxin derived modifications specific activity. It was experimentally determined that diphtheria toxin modifications obtained by the instrumentality of modifier number 1 with or without the physical factor processing were safe and non-toxic.
Key words: diphtheria toxin, toxoid, chemical factors, physicochemical factors



5-8

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SPECIFIC DIFFERENTIATION AND EPIDEMIOLOGICAL MARKING OF STAPHYLOCOCCUS AUREUS STRAINS DISTINGUISHED FROM THE CARRIERS OF MEDICAL PERSONNEL AND OBJECTS OF EXTERNAL ENVIRONMENT IN CURATIVE INSTITUTIONS OF THE SOUTH RAILWAY
Rudenko S.S., Korobkova I.V., Sobol .M., Astapova V.V., Pivnenko T.V., Grechishkina Y.A., Kanives E.A.
Carried out specific differentiation of pathogenic strains of Staphylococcus aureus, which were isolated from various environmental objects surgical hospital, biomaterials from patients. The results of the research brought together strains of pathogenic staphylococci in the lytic group and 4 show the frequency of detection of each analytic group in the particular material. Received the rationale for the introduction of phage-typing method in the  practical  work  of  bacteriological  laboratories,  epidemiological  control  software  in  the  hospital  for bacteriological indicators to effectively combat nosocomial infections.
Key  words:  S.aureus (golden Staphylococcus), nosocomial diseases, treatment-and- prophylactic establishments of medical service of the Southern railway,  surgical hospitals, postoperative  pyo-inflammatory complications, phagotyping (identified strains microorganism with the set of international specific phages)


9-13

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OPTIMIZATION OF CONDITIONS THE CULTIVATION FOR HYALURONIDASE PRODUCTION BY STREPTOCOCCUS PYOGENES
Lukyanenko T.V.
Hyaluronidase   is   used   in   biotechnology   processes   and  medicine.  A  lot  of  the  preparations  of hyaluronidase produce from animal source with limited sources of microbial origin. Bacterial hyaluronidase were  reported  to be virulence factors that facilitate the spreading of bacteria in host tissues by degradation of hyaluronic  acid.  This  article  included  the  results  of  studies  by  the  choice  of  optimal  conditions  of  the cultivation for hyaluronidase production by teststrains  S.  pyogenes.  The  higher HA detected  from the strains  from  the  patients  with  pneumonia.  Optimum  values  of  ,  temperature  and  cultivation  term  of microorganisms determined,  an  influence of components of nutrient   mediums on enzyme production was studied. Study of HA of GAS shows the increase in enzyme activity in medium containing glucose, hyaluronic acid, serum and their combination. For increase in activity we recommended calcium gluconate and quaternary ammonium compound at the concentration.
Key worlds: hyaluronidase, S. pyogenes, conditions cultivation.

14-19

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THE STATE OF ANTIMEASLES IMMUNITY IS IN OFTEN BEING ILL CHILDREN AND INTERCOMMUNICATION OF LEVEL OF ANTIMEASLES ANTIBODIES WITH HLA-PHENOTYPE
Usatiy R. S. , Popov N. N. , Romanova E. A., Savvo .N.

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Conducting this study provided an opportunity to assess the  state  of  specific  immunity  in  measles  sickly children,  as  well  as  to  detect  and  analyze  the relationship  measles  IgG  titer  with  the  level  of individual classes of immunoglobulins serum blood and HLA-phenotypes.   The  data  indicate  that  the development  of  measles  IgG-antibodies  is  in  close contact with  the  general  immunoreactivity  body  and directly  correlated  with  the  level  of  total immunoglobulin G and affinity gG that is produced by CAD of  the bacteries.  Also  during  the  operation revealed that the low intensity of measles immunity is associated with a complex of HLA-DR7 and HLA-DR Blank. Results can be used to predict the  effectiveness of  vaccination,  the  timing  and  modalities  of  its implementation.
Key words:measles, vaccination, often ill children.



20-23

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ANALYSIS OF SOME INDICATORS CELLULAR AND HUMORAL IMMUNITY IN CHILDREN WITH BRONCHIAL ASTHMA
Usenko S.G., Usenko S.A.

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In children with atopic asthma were studied indexes of specific cellular and humoral immunity in the dynamics: when receiving to the clinic before discharge and after 3 months  of  remission. Changes  in  some  immunological parameters are phase nature. In the period of intensifying of disease a  lymphopenia that was expressed in the decline of amount of and B of cells were marked. Except it, the disbalance of immunoregulatory subpopulations of T-cell was marked,  that showed up in the decline of -helper,  and  in  the  increase  of  T-suppressor-cell  for children  that  are  ill  atopic  bronchial  asthma. Disimmunoglobulinaemia  consisted  in  the  decline  of concentration of Ig A, increase of concentration of Ig M and  Ig  G.  The  period  of  clinical  recovery  was accompanied by normalization of immune status of minor children. Not  fully recovered rate of cellular  immunity link.
Key words: bronchial asthma, immunity, children.


24-29

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UKRAINIAN FOUNTAINS OPEN HEARTH INFECTIOUS DISEASES: CAUSES, SITUATION ANALYSIS, SOLUTION
Martynov A.V., Manuilov M.B., Stepanova I.I., Mankovsky V.V., Moskovkin V.M.
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The paper examined the causal links that explain the pattern of contamination of water systems of water recycling fountains by pathogens and proved the rreversibility of the formation of the path of infection of domestic  population  of  by  airborne  droplets,  formed  by  microbial  fogs.  Studies  of  microbiolodical contamination of water allowed to obtain informative features for preliminary analysis of epidemiological danger of fountains and provided an opportunity to identify the dominant factor high levels of Mycobacterium tuberculosis in aerosol droplets. On the basis of the collected databases were formulated technical conditions to ensure the security of the collected databases were formulated technical conditions to ensure the security of citizens from all possible negative impacts of fountains, realized in the form of water disinfection technology working with silver and copper, which consistent with international sanitary rules, regulations Ministry of Health Care of Ukraine and environmental legislation.
Key words: fountains, infection, inactivation, silver copper electrolysis

30-38

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ANALYSIS OF ANTIBIOTIC SENSITIVITY OF CLINICAL STRAINS, ISOLATED IN SURGICAL AND UROLOGIC HOSPITALS OF IVANO-FRANKIVSK
Frych N.I.

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The sensitivity to antibiotics of various groups of 7903 bacterial strains, isolated from patients with purulent and urological infections in Ivano-Frankivsks hospitals is  presented. The causative agents of urinary, wound and purulent infections are Gram-positive bacteria, especially CNS (33,1%), Staphylococcus aureus (29,1 %), and in 35,5 % of cases Gram-negative bacteria Esherichia coli (20,4 %), Pseudomonas aeruginosa (7,6 %) and Proteus spp (5,1 %). The regional profiles of drug resistance of wound and urinary infections causative agents was established.
Key words: opportunistic microorganisms, opportunistic infections, antibiotic resistance.


39-47

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ANTIMICROBIAL ACTIVITI OF EXTRACTS FROM THE LEAVES AND BARK OF BRANCHES OF  THE ORNELIAN CHERRY DOGWOOD
Krivoruchko Ye.V., Shulga N.N., Samoylova V.A., Kovalyov V.N.
It were found antimicrobial activity to S. pneumoniae, S. aureus, P. aeruginosa, K. pneumoniae, B. anthracoides and E. coli of extracts from leaves and bark of branches of ornelian cherry dogwood (Cornus mas L.) using the agar diffusion method. It was not found antifungal activity to C. albicans. Tincture of the leaves (40 and 70% alcohol) have higher antimicrobial activity than the tincture of the bark of branches (40%) and dense aqueous extracts of leaves and bark of branches of the ornelian cherry dogwood.
Key words: antimicrobial activity, extract, ornelian cherry dogwood, Cornus mas L.


48-50

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THE ABUNDANCE OF CANDIDIS, THE GENERAL CHARACTERISTICS OF THE PATOGEN, APPROACHES TO THE LABORATORU DIAGNOSTIC
Holubka O.V.

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The article contains material devoted to the problem of the Candida infection, which is becoming more significant due to its spreading among the population; it covers the issues about the causes of this pathology, among which the most significant is the prescribing of antibiotics therapy, using of hormones, immune suppressants, cytostatics. Based on the research works of native and foreign scholars, the modern attitude to the problem of the early laboratory diagnosis, and, therefore, prompt and justified prescribing of antifungal therapy is presented in the article. The publication contains data about the pathogenic potential of the genus Candida fungi. It focuses clinicians and bacteriologists on searching new and improving existing methods of the early and qualified laboratory diagnosis of the Candida infection.
Key words: genus Candida fungi, the Candida infection, the laboratory diagnosis of the candidiasis.
51-59

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(History of science)



THE HISTORY OF ANTIPHTHISIC SERVICE OF THE KHARKIV REGION AND THE DYNAMICS OF THE EPIDEMIOLOGICAL SITUATION OF TUBERCULOSIS IN SLOBOZHANSCHYNA
Kovalyova G.O., Sencheva T.V., Petrushov A.V., Bondar V.O., Kuchma I.Y., Volyansky A.Y., Martirosyan I.O., Baluta I.M.

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Description of the stages of antiphthisic service of Khar'kovschiny and its modern state is resulted in the article, priority work assignments are considered, the dynamics of pidemiology situation is resulted on tuberculosis
Key words: antiphthisic service, dispensary, diagnostics, treatment, DOTS-strategy, program

60-64

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I. I. MECHNIKOV AND HIS WORKS ABROD
Galushk R.A., Kuchma I.Yu, Glazunova L.I.

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The main scientific activity of  I.  I. Mechnikov has been carried out abroad since 1864. At  first  it was periodical, then since 1888 until his death in 1916 he was working abroad  permanently.  He  developed some   aspects   of  comparative  and  evolutional  embryology  and  proposed  an  original   theory   of multicellular   animals   origin.  He   also  established   comparative   pathology   of   inflammation   and hereafter   the   phagocytic   theory   of   immunity.   He   also  dedicated  much   of   his   time   to bacteriology:   he   studied aspects   of   epidemiology   of   cholera,    typhoid   fever, tuberculosis and other infectious diseases and also scientific gerontology.  The residence at Institut Pasteur for 28 years was    a period  of  fruitful work  and  general  acclaim for Mechnikov.
Key words: Mechnikov, work abroad, scientific labours.

65-69

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