Ç̲ÑÒ (Contents)
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C. (P.) |
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Ðåäàêö³éíà ðàäà (Editorial Board) |
1 |
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Çì³ñò (Contents) |
2-4 |
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| ÅÊÑÏÅÐÈÌÅÍÒÀËÜͲ
ÐÎÁÎÒÈ (EXPERIMENTAL STUDY) |
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| DETERMINATION OF REGIMES FOR DIPHTHERIA EXOTOXIN MODIFICATION BY CHEMICAL AND PHYSICOCHEMICAL METHODS Babych E.M., Kalinichenko S.V., Ryzhkova T.A., Sklyar N.I., Ryabovol O.V., Pluhator T.M., Antusheva T.I., Panova C.V. The
possibility of diphtheria toxoid obtaining using chemical (amino
sugars, organic acids) and physicochemical (amino sugars, organic
acids, ultrasound, temperature) factors was studied. It was established
that modifiers (including formaldehyde) volume content decreasing
didn’t have significant influence on diphtheria toxin derived
modifications specific activity. It was experimentally determined that
diphtheria toxin modifications obtained by the instrumentality of
modifier number 1 with or without the physical factor processing were
safe and non-toxic.
Key words: diphtheria toxin, toxoid, chemical factors, physicochemical factors |
5-8 |
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| SPECIFIC
DIFFERENTIATION AND EPIDEMIOLOGICAL MARKING OF STAPHYLOCOCCUS AUREUS
STRAINS DISTINGUISHED FROM THE CARRIERS OF MEDICAL PERSONNEL AND
OBJECTS OF EXTERNAL ENVIRONMENT IN CURATIVE INSTITUTIONS OF THE SOUTH
RAILWAY Rudenko S.S., Korobkova I.V., Sobol Î.M., Astapova V.V., Pivnenko T.V., Grechishkina Y.A., Kanives E.A. Carried
out specific differentiation of pathogenic strains of Staphylococcus
aureus, which were isolated from various environmental objects surgical
hospital, biomaterials from patients. The results of the research
brought together strains of pathogenic staphylococci in the lytic group
and 4 show the frequency of detection of each analytic group in the
particular material. Received the rationale for the introduction of
phage-typing method in the practical work of
bacteriological laboratories, epidemiological
control software in the hospital for
bacteriological indicators to effectively combat nosocomial infections.
Key words: S.aureus (golden Staphylococcus), nosocomial diseases, treatment-and- prophylactic establishments of medical service of the Southern railway, surgical hospitals, postoperative pyo-inflammatory complications, phagotyping (identified strains microorganism with the set of international specific phages) |
9-13 |
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| OPTIMIZATION OF CONDITIONS THE CULTIVATION FOR HYALURONIDASE PRODUCTION BY STREPTOCOCCUS PYOGENES Lukyanenko T.V. Hyaluronidase
is used in
biotechnology processes and
medicine. A lot of the preparations
of hyaluronidase produce from animal source with limited sources of
microbial origin. Bacterial hyaluronidase were reported to
be virulence factors that facilitate the spreading of bacteria in host
tissues by degradation of hyaluronic acid. This
article included the results of
studies by the choice of optimal
conditions of the cultivation for hyaluronidase production
by teststrains S. pyogenes. The higher HA
detected from the strains from the
patients with pneumonia. Optimum values
of ðÍ, temperature and cultivation
term of microorganisms determined, an influence of
components of nutrient mediums on enzyme production was
studied. Study of HA of GAS shows the increase in enzyme activity in
medium containing glucose, hyaluronic acid, serum and their
combination. For increase in activity we recommended calcium gluconate
and quaternary ammonium compound at the concentration.
Key worlds: hyaluronidase, S. pyogenes, conditions cultivation.
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14-19 |
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| THE
STATE OF ANTIMEASLES IMMUNITY IS IN OFTEN BEING ILL CHILDREN AND
INTERCOMMUNICATION OF LEVEL OF ANTIMEASLES ANTIBODIES WITH HLA-PHENOTYPE Usatiy R. S. , Popov N. N. , Romanova E. A., Savvo À.N. ÑÒÀÍ ÏÐÎÒÈÊÎÐÎÂÎÃÎ ²ÌÓͲÒÅÒÓ Ó ×ÀÑÒÎ ÕÂÎвÞ×ÈÕ Ä²ÒÅÉ ÒÀ ÂÇÀªÌÎÇÂ'ßÇÎÊ Ð²ÂÍß ÏÐÎÒÈÊÎÐÎÂÈÕ ÀÍÒÈÒ²Ë Ç HLA-ÔÅÍÎÒÈÏÎÌ Óñàòèé Ð. Ñ. ,Ïîïîâ Ì. Ì. , Ðîìàíîâà Î. À., Ñàââî Î.Ì. Conducting
this study provided an opportunity to assess the state
of specific immunity in measles sickly
children, as well as to detect
and analyze the relationship measles IgG
titer with the level of individual classes of
immunoglobulins serum blood and HLA-phenotypes. The
data indicate that the development of
measles IgG-antibodies is in close contact
with the general immunoreactivity body
and directly correlated with the level
of total immunoglobulin G and affinity ²gG that is produced by
CAD of the bacteries. Also during the
operation revealed that the low intensity of measles immunity is
associated with a complex of HLA-DR7 and HLA-DR Blank. Results can be
used to predict the effectiveness of vaccination,
the timing and modalities of its
implementation.
Key words:measles, vaccination, often ill children. |
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| ANALYSIS OF SOME INDICATORS CELLULAR AND HUMORAL IMMUNITY IN CHILDREN WITH BRONCHIAL ASTHMA Usenko S.G., Usenko S.A. ÀÍÀË²Ç ÎÊÐÅÌÈÕ ÏÎÊÀÇÍÈʲ Ê˲ÒÈÍÍÎÃÎ ÒÀ ÃÓÌÎÐÀËÜÍÎÃÎ ²ÌÓͲÒÅÒÓ Ó Ä²ÒÅÉ, ÕÂÎÐÈÕ ÍÀ ÁÐÎÍÕ²ÀËÜÍÓ ÀÑÒÌÓ Óñåíêî Ñ.Ã., Óñåíêî Ñ.À In
children with atopic asthma were studied indexes of specific cellular
and humoral immunity in the dynamics: when receiving to the clinic
before discharge and after 3 months of remission.
Changes in some immunological parameters are phase
nature. In the period of intensifying of disease a lymphopenia
that was expressed in the decline of amount of Ò and B of cells were
marked. Except it, the disbalance of immunoregulatory subpopulations of
T-cell was marked, that showed up in the decline of
Ò-helper, and in the increase of
T-suppressor-cell for children that are
ill atopic bronchial asthma.
Disimmunoglobulinaemia consisted in the
decline of concentration of Ig A, increase of concentration of Ig
M and Ig G. The period of
clinical recovery was accompanied by normalization of
immune status of minor children. Not fully recovered rate of
cellular immunity link.
Key words: bronchial asthma, immunity, children. |
24-29 |
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| UKRAINIAN FOUNTAINS OPEN HEARTH INFECTIOUS DISEASES: CAUSES, SITUATION ANALYSIS, SOLUTION Martynov A.V., Manuilov M.B., Stepanova I.I., Mankovsky V.V., Moskovkin V.M. ÔÎÍÒÀÍÛ ÓÊÐÀÈÍÛ ÎÒÊÐÛÒÛÅ Î×ÀÃÈ ÈÍÔÅÊÖÈÎÍÍÛÕ ÇÀÁÎËÅÂÀÍÈÉ: ÏÐÈ×ÈÍÛ, ÀÍÀËÈÇ ÑÈÒÓÀÖÈÈ, ÐÅØÅÍÈÅ ÏÐÎÁËÅÌÛ Ìàðòûíîâ À.Â., Ìàíóéëîâ Ì.Á.,Ñòåïàíîâà È.È., Ìàíüêîâñêèé Â.Â., Ìîñêîâêèí Â.Ì. The paper
examined the causal links that explain the pattern of contamination of
water systems of water recycling fountains by pathogens and proved the
rreversibility of the formation of the path of infection of
domestic population of by airborne
droplets, formed by microbial fogs.
Studies of microbiolodical contamination of water allowed
to obtain informative features for preliminary analysis of
epidemiological danger of fountains and provided an opportunity to
identify the dominant factor – high levels of Mycobacterium
tuberculosis in aerosol droplets. On the basis of the collected
databases were formulated technical conditions to ensure the security
of the collected databases were formulated technical conditions to
ensure the security of citizens from all possible negative impacts of
fountains, realized in the form of water disinfection technology
working with silver and copper, which consistent with international
sanitary rules, regulations Ministry of Health Care of Ukraine and
environmental legislation.
Key words: fountains, infection, inactivation, silver – copper electrolysis |
30-38 |
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| ANALYSIS OF ANTIBIOTIC SENSITIVITY OF CLINICAL STRAINS, ISOLATED IN SURGICAL AND UROLOGIC HOSPITALS OF IVANO-FRANKIVSK Frych N.I. ÀÍÀË²Ç ×ÓÒËÈÂÎÑÒ² ÄÎ ÀÍÒÈÁ²ÎÒÈʲ Ê˲Ͳ×ÍÈÕ ØÒÀ̲ ̲ÊÐÎÎÐÃÀͲÇ̲Â, ÂÈIJËÅÍÈÕ Â Õ²ÐÓÐò×ÍÈÕ ÒÀ ÓÐÎËÎò×ÍÈÕ ÑÒÀÖ²ÎÍÀÐÀÕ ì. ²ÂÀÍÎ-ÔÐÀÍʲÂÑÜÊÀ Ôðè÷ Í.². The
sensitivity to antibiotics of various groups of 7903 bacterial strains,
isolated from patients with purulent and urological infections in
Ivano-Frankivsks hospitals is presented. The causative agents of
urinary, wound and purulent infections are Gram-positive bacteria,
especially CNS (33,1%), Staphylococcus aureus (29,1 %), and in 35,5 %
of cases – Gram-negative bacteria – Esherichia coli (20,4 %),
Pseudomonas aeruginosa (7,6 %) and Proteus spp (5,1 %). The regional
profiles of drug resistance of wound and urinary infections causative
agents was established.
Key words: opportunistic microorganisms, opportunistic infections, antibiotic resistance. |
39-47 |
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| ANTIMICROBIAL ACTIVITI OF EXTRACTS FROM THE LEAVES AND BARK OF BRANCHES OF THE ÑORNELIAN CHERRY DOGWOOD Krivoruchko Ye.V., Shulga N.N., Samoylova V.A., Kovalyov V.N. It were
found antimicrobial activity to S. pneumoniae, S. aureus, P.
aeruginosa, K. pneumoniae, B. anthracoides and E. coli of extracts from
leaves and bark of branches of Ñornelian cherry dogwood (Cornus mas L.)
using the agar diffusion method. It was not found antifungal activity
to C. albicans. Tincture of the leaves (40 and 70% alcohol) have higher
antimicrobial activity than the tincture of the bark of branches (40%)
and dense aqueous extracts of leaves and bark of branches of the
Ñornelian cherry dogwood.
Key words: antimicrobial activity, extract, Ñornelian cherry dogwood, Cornus mas L. |
48-50 |
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| THE ABUNDANCE OF CANDIDIS, THE GENERAL CHARACTERISTICS OF THE PATOGEN, APPROACHES TO THE LABORATORU DIAGNOSTIC Holubka O.V. ÏÎØÈÐÅͲÑÒÜ ÊÀÍÄÈÄÎDzÂ, ÇÀÃÀËÜÍÀ ÕÀÐÀÊÒÅÐÈÑÒÈÊÀ ÇÁÓÄÍÈÊÀ, ÎÑÎÁËÈÂÎÑÒ² ËÀÁÎÐÀÒÎÐÍί IJÀÃÍÎÑÒÈÊÈ Ãîëóáêà Î.Â. The
article contains material devoted to the problem of the Candida
infection, which is becoming more significant due to its spreading
among the population; it covers the issues about the causes of this
pathology, among which the most significant is the prescribing of
antibiotics therapy, using of hormones, immune suppressants,
cytostatics. Based on the research works of native and foreign
scholars, the modern attitude to the problem of the early laboratory
diagnosis, and, therefore, prompt and justified prescribing of
antifungal therapy is presented in the article. The publication
contains data about the pathogenic potential of the genus Candida
fungi. It focuses clinicians and bacteriologists on searching new and
improving existing methods of the early and qualified laboratory
diagnosis of the Candida infection.
Key words: genus Candida fungi, the Candida infection, the laboratory diagnosis of the candidiasis. |
51-59 |
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²ñòîð³ÿ íàóêè (History of science) |
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| THE
HISTORY OF ANTIPHTHISIC SERVICE OF THE KHARKIV REGION AND THE DYNAMICS
OF THE EPIDEMIOLOGICAL SITUATION OF TUBERCULOSIS IN SLOBOZHANSCHYNA Kovalyova G.O., Sencheva T.V., Petrushov A.V., Bondar V.O., Kuchma I.Y., Volyansky A.Y., Martirosyan I.O., Baluta I.M. ²ÑÒÎÐ²ß ÏÐÎÒÈÒÓÁÅÐÊÓËÜÎÇÍί ÑËÓÆÁÈ ÕÀÐʲÂÑÜÊί ÎÁËÀÑÒ² ÒÀ ÄÈÍÀ̲ÊÀ ÅϲÄÅ̲ÎËÎò×Íί ÑÈÒÓÀÖ²¯ Ç ÒÓÁÅÐÊÓËÜÎÇÓ ÍÀ ÑËÎÁÎÆÀÍÙÈͲ Êîâàëüîâà Ã.Î., Ñåí÷åâà Ò.Â., Áîíäàð Â.Î., Êó÷ìà ².Þ., Âîëÿíñüêèé À.Þ., Ìàðò³ðîñÿí ².Î., Áàëóòà ².Ì. Description
of the stages of antiphthisic service of Khar'kovschiny and its modern
state is resulted in the article, priority work assignments are
considered, the dynamics of pidemiology situation is resulted on
tuberculosis
Key words: antiphthisic service, dispensary, diagnostics, treatment, DOTS-strategy, program |
60-64 |
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| I. I. MECHNIKOV AND HIS WORKS ABROD Galushkà R.A., Kuchma I.Yu, Glazunova L.I. ÍÀÓÊÎÂÀ IJßËÜͲÑÒÜ ².². ÌÅ×ÍÈÊÎÂÀ ÇÀ ÊÎÐÄÎÍÎÌ Ãàëóøêà Ð.À., Êó÷ìà ².Þ, Ãëàçóíîâà Ë.². The main
scientific activity of I. I. Mechnikov has been carried out
abroad since 1864. At first it was periodical, then since
1888 until his death in 1916 he was working abroad
permanently. He developed some
aspects of comparative and
evolutional embryology and proposed an
original theory of multicellular
animals origin. He also
established comparative pathology
of inflammation and hereafter
the phagocytic theory
of immunity. He also
dedicated much of his
time to bacteriology: he studied
aspects of epidemiology
of cholera, typhoid fever,
tuberculosis and other infectious diseases and also scientific
gerontology. The residence at Institut Pasteur for 28 years
was a period of fruitful work
and general acclaim for Mechnikov.
Key words: Mechnikov, work abroad, scientific labours. |
65-69 |
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