Annals of Mechnikov's Institute 2006 3
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(REVIEWS)



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CURRENT STAGE IN THE DEVELOPMENT OF CHEMICAL VACCINES FOR THE PREVENTION OF PSEUDOMONAS INFECTION
Gorodnytska N.I., Yasnaya N.S., Martynov A.V., Chernyavsky V.I., Kozubova G.M., Smelyanska M.V., Osolodchenko T.P., Peremot S.D, Romanova E.A., Igumnova N.I., Sydorenko T.O.
In the paper was described the current stage of development the Pseudomonas infection vaccine. Both the advantages and weak points of various vaccine preparations are thoroughly analyzed. It is noted that no oral monocomponent vaccine has been developed so far, although the metabolic exoproducts of the Pseudomonas aeruginosa strains listed in this paper should be considered as those having rather great potential for being used as components for vaccine preparations to be developed. Therefore, we propose to produce the reguired monocomponents from metabolic exoproducts of Pseudomonas aeruginosa strains though inactivating them by ultrasound, high temperature and ultraviolet irradiation, then to single out more immunogenic components, to modify them chemically, to hydrolyze the high-molecular components, and to obtain more advantageous formula of monocomponent structure to be used for making vaccine preparations. Then it is necessary to test the revealed compositions on animals in order to study their immunogenicity and to standardize antigens if they are immunogenic in case of peroral administration.
Key words : Pseudomonas infection, metabolic exoproducts of Pseudomonas aeruginosa strains, vaccine development.

4-17

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BIOSENSORS
Jelali V.V., Volyansky A.Y.
In the review, there are presented characteristics of processes of electronic and ionic transport, analysis of mechanisms of generation of electrical potential by cell membranes and its transformation as a result of as a result of cell activity. The basic principles of registration of electrochemical signals from microbiological objects are considered. The basic directions of research and designing of biosensoric systems have been analysed.
Key words: biosensor, impedance spectroscopy, energy Fermi , membrane, crate.
 
18-34

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HYDROXYAPATITE DEPOSITION FROM AQUEOUS SOLUTION ONTO TIALV AND TIALV COVERED WITH COLLAGEN BY THE THERMAL SUBSTRATE METHOD
Yu.L. Volianskiy, B. Sulkio-Cleff, V.V.Pilipenko, L.B. Sukhodub, L.F. Sukhodub.
Thermal substrate method recently proposed for getting hydroxyapatite coating on titanium was used to obtain deposition of high crystallinity hydroxyapatite onto titanium-based plate (TiAlV) on physiological temperature. The coating was produced onto free plates and onto collagen covered surfaces. The collagen was introduced to surface of titanium-based plates in order to modulate possibility to get bioactive layers of hydroxyapatite by the thermal substrate method, what is important for improving osteointegration of titanium implants into bone. The influence of pH value of solution on the processes of deposition was investigated. The experimental conditions were: temperature = 37 C, deposition time = 1 hour, pH = 6,00 - 6,3 and Ca/P = 1,67. The best pH value for depositing high crystallinity hydroxyapatite on target corresponding to 6,15, going down with pH reducing speed of deposition and increasing pH to 6,3 decrease crystallinity of hydroxyapatite. Collagen improved speed and crystallinity of layers.
Key words: thermal substrate method, hydroxyapatite, and collagen.

35-39

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STUDY OF MICROORGANISMS SENSITIVITY TO ANTIBIOTICS, THAT WAS CULTIVATED ON MEDIUMS ON BASE OF ERYTHROCYTES MASS
Batrak E. A., Zavada N. P., Rjabova I.S., Shtiker L.G.
There was studied an antimicrobial activity of antibiotics on a standard nutrient medium AGB in comparison with experimentally developed medium which contained packed red blood cells of overdued working life. The antimicrobial activity of the medications under circumstances of the medium obtained by the method of acid hydrolysis of packed red blood cells was lower than under circumstances of the standard nutrient medium AGB. By means of addition of the growth ingredients (glucose, peptone, serum) it is planned to achieve an improvement of growth properties of microorganisms.
Key words: antibiotics, microorganisms, medium, erythrocytes mass (from donors blood)

40-44

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MICROBIOLOGICAL ASPECTS OF PERSISTENCE OF CORYNEBACTERIUM DIPHTHERIAE (THE REVIEW)
Ryzhkova T.A.
The description of modern diphtheritic infection's epidemic process in Ukraine is presented in the article. The possibility of intercommunication between diphtheria morbidity, carriage of Corynebacterium diphtheriae and nonspecific pathology of ear, nose, throat is retraced. The structure and mutual relations between representatives of microbial population at diphtheria patients and at diphtheria carriers are characterized. The data of the influence of low oxygen concentrations on biological properties and mutual relations between members in microbial associations of tonsillar tissue are adduced.
Key words: diphtheria, Corynebacterium, microbial associations, antagonistic activity, pathology of ear, nose, throat.

45-55

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SOME QUESTIONS IN PROBLEMS OF ACUTE INTESTINAL INFECTION AND MICROECOLOGY
Voronkina I.A.
The problem of intestinal infections remains actual and for our time. Among acute intestinal infections one of widespread are shigellosis and salmonellosis. In post-war years on USSR territory the chronic motion of intestinal infections was registered. With appearance of antibiotics they chronic forms was disappeared, but other problems remains: appearance of new antibiotic - resistant microorganisms strains. Morbidity of shigellosis has a tendency to growth not only on Ukraine but also in other countries of Europe and America.
Keywords: acute intestinal infections, shigellosis, salmonellosis, morbidity, antibiotic - resistant microorganisms.

56-60

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BIOTECHNOLOGY OF THE DIPHTHERIA PRECIPITATE SERUM RECEIPTION
Kaliagina S. Yu., Ikramov A.A.
New antigenic preparations from C. diphtheriae represent the big scientific - practical interest. A biomass of one toxi-genic and two nontoxigenic strains C. diphtheriae received a method deep cultivation on experimental nutrient medium, after selection of an optimum mode of aeration and hashing. Inactivation of biomass of diphtheritic bacteria made by hydroxylamin hydrochloride. Soluble antigenic preparations allocated with a method of convection and liophilic dried up. For an antigenic preparation from toxigenic strain C. diphtheriae studied toxicity on white mice and antigenic activity at immunization of rab-bits. The received whey tested in reaction precipitation with gomologic antigene, with antigenes from nontoxigenic strains and diphtheritic anatoxin.
 
61-69

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(DIPTERA: CERATOPOGONIDAE)
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FORMATION OF MIDGES (DIPTERA: CERATOPOGONIDAE) FAUNA IN VARIOUS POND TYPES OF KHARKOV AREA
Prudkuna N.S., Mushchenko A.A., Mashkey A.N., Solodiankin A.S.
In the article, results of long-term ecological-faunistic researches in territory of the Kharkov Area are presented. More than 30 species of midges are registered. Places of mass breeding are typified.
Keys words: midges, blood-sucking insects, Kharkov Area, ponds

70-73

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METHODOLOGICAL ASPECTS OF INVESTIGATION OF INFECTIOUS DISEASES IN P. KOH`S AND L. PASTER`S WORKS
Pugach N.
In the article the author pays attention to insufficiently studied sides of activity of scientists P.Kokh and L.Paster. They made decisive contribution in development of modern microbiology and immunology, creation of new conception of microbes world. The author shows complicated way of investigation of Antrax aetiology, picks out main methods, principles of prophylaxis of infectious diseases. In generalize form the author formulates demands of theory of knowledge of pathogenic microbes.
74-101

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