Annals of Mechnikov's Institute 2007 3
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(Contents)

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(REVIEWS)




QS-DEPENDENT VIRULENCE REGULATION IN PSEUDOMONAS AERUGINOSA
Krestetska S.L., Nesterenko A.M.

QS-DEPENDENT VIRULENCE REGULATION IN PSEUDOMONAS AERUGINOSA
Krestetska S.L., Nesterenko A.M.
The discovery of the cell-to-cell communication in the bacterial populations led to the understanding that prokaryotes are capable of coordinated activity. That facilitates development of a very attractive field of research, within which exists ability to reveal fundamentally new patterns that control course of events during bacterial infection, and, appropriately, preconditions for development of new therapeutical approaches also exists. For the recent years different kinds of quorum sensing systems, that support the perception of population quantitative conditions and ensure its application for synchronisation of the certain gene expression, were described in different species of microorganisms. One of the most studied objects is P.aeruginosa. Its adaptive potential provides the ability to exist in different environmental conditions, to infect a wide range of the organisms (from Protozoa to Homo sapiens) and to modulate population behaviour aggressiveness in compliance with its suitability degree.
Keywords: bacterial populations, cell-to-cell communication, quorum sensing, P.aeruginosa

4-7

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MODERN APPROACHES TO VIABILITY ASSESSMENT OF BACTERIA WITH ACCENT ON NONCULTURABILITY PHENOMENON
Yudin I.P.
Problems of bacteria viability determination were considered from the point of view of opportunity of them surviving in a nonculturable state. Traditionally, since appearance of microbiology as sciences, methods of cultivation were and, by and large, remain "the gold standard" for bacteriologists. However, demands to accuracy and reliability at detection of pathogenic bacteriums, to determination of their viability now have considerably increased.
Key words: viability, viable but nonculturable (VBNC), nonculturable, fluorescence.
 
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(EXPERIMENTAL STUDY)




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SOME FEATURES OF IMMUNE STATUS UNDER THE IMPACT OF DIFFERENT IONIZING IRRADIATION DOSES
Kolyada T.I., Brusnik S.V., Andreeva I.D., Nesterenko A.M., Sherbak O.N.,
Mikhailichenko M.S.
Ionizing irradiation causes a complex profound metabolic impairment of the organism, that affects the immune system. The variation of irradiation dose and its power, as well as the influence of irradiation on the part of the organism, leads to the specific changes of the immunologic manifestations. One of the leading causes of the immunological impairment is the development of the thymic function depression, that causes deeper changes in the cellular im munity compared to the humoral immunity.
Keywords: ionizing radiation, radiosensitivity, immune system, immunodeficiency, immunocopetent cells, immunocopetent organs.

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DETECTION OF MARKERS OF ACUTE HERPESVIRUS PROCESS, ASSOCIATED WITH HERPES SIMPLEX AND EPSTEIN-BARR HERPES VIRUSES IN PATIENTS WITH ACUTE AND CHRONIC LARYNGITIS
Panchenko L.A., Torianik I.I., Popova N.G., Kandzuba S.I., Popova L.A.
Markers of acute HSV infection (Ig M to HSV) were detected in the blood of 108 patients, including 47.5 % of patients with acute and 26.4 5 % of patients with chronic laryngitis. Acute EBV infection markers (VCAIg M and EA-Ig G) were detected in 6 from the 23 (26.6 %) examined patients.
Key words: Herpes simplex virus, Epstein-Barr virus, acute and chronic laryngitis.

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˲ ֲ ˲ ˲ ²ί ֲ NICOTIANA TABACUM
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INFLUENCE OF HETEROPOLYNUCLEAR COORDINATE COMPOUNDS OF METALS AND SALTS OF HEAVY METALS ON THE DEVELOPMENT OF VIRAL INFECTION IN PLANTS OF NICOTIANA TABACUM
Korniichuk I, Ustyanenko D., Kot T., Kharina A., Polishuk V.
The antiphytoviral activity of heteropolynuclear compounds of metals 201, 399, 417 and salts of heavy metals on the model system tobacco mosaic virus (TMV) - Nicotiana tabacum was studied. The substance H417 showed the largest influence on the TMV infectivity. It inhibited the infectivity of tobacco mosaic virus for 40%. While studying the main contents of some complex substances of heavy metals the activity of NiCl2 × 6H2O was showed. The substance inhibited the development of callus for 36%.

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IMMUNE STATUS OF CHILDREN WITH ROTAVIRAL MIXED INFECTION
Kuznetsov S.V., Kirsanova T.A.
The article presents the results of the study of cytokine status and immune response of 64 children with rotaviral mixed infection. Investigation shows that acute period of rotaviral mixed infection accompanied by reliable increase of interleukines -1b, -4, -6 and tumor necrosis factor a. In early reconvalescence period the level of these interleukines stays increased. The immune responce of rotaviral mixed infection is characterized by decreased level of D3+, D4+, D8+ lymphocytes at the background of physiological level of D14+, increased level of D19+ during the whole period of the disease, physiological level of antibodies A, M, G in acute period and increased level of antibody G in early reconvalescence period. Mathematically proven fact is children with rotaviral mixed infection has hypocompensatory variant of operation of immunity.
Key words: rotaviral mixed infection, immunity, children.
 

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SPECIES COMPOCITION AND ANTIBIOTICS SENSIBILITY OF BILES MICROFLORA OF THE PATIENTS WITH INFLAMMATORY PATOLOGY OF BILIARY TRACT
Diachenco V.F. Mariushchenko A.M., Artiomov Yu.V., Yagniuk Yu.A., Bacumenko A.V., Yagniuk A.I., Piatacov A.V.
The bacteriocholia has been revealed at 91,3 % investigated patients with acute and chronic inflammatory diseases of biliar tract. Species composition of biliar microflora is presented first of all the enterobacteria and the bacteroides. The major part of separated from the bile microorganisms was resistant to the antibiotics, that are used traditionally in surgical practice for the treatment and prophylaxis of infections complications.
Key words: cholecystitis, cholangitis, cholecystectomia, bacteriocholia, antibioticoresistance.
 
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INFLUENCE DIPHTHERIAL AND TANATOXINS ON NTIBODYGENESIS AT RATS OF DIFFERENT AGE
Volyanskiy A.Y., Simirenko L.L., Kuchma I.Y., Misin V.V., Nikitchenko Yu.V.,
Davidenko M.B.

Investigated process of formation of the humoral immune answer on diphtheria and T-anatoxin at 3- and 20- month old male Wistar rats. Fixed, that irrespective of age the immunization by a diphtherial anatoxin was accompanied by more appreciable level specific antibodygenesis, than at an animal under influence T-anatoxin.
Key words: antidiphtherial antibodies, diphtherial anatoxin, DT-anatoxin, rats, age.

31-33

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BIOLOGICAL PROPERTIES OF MICRORGANISMS CULTIVATED ON NUTRITIOUS MEDIUM RESEIVED FROM INDUSTRY WASTE
solodchenko .P
In work the opportunity use for reception of a nutritious basis for microbiological nutrient mediums by acid hydrolysis is shown. The estimation properties of the received nutrient mediums on the basis of studying process of cultivation microorganisms and biochemistry properties in comparison with standard nutrient mediums is given.
Key words: nutritious basis, medium, microorganisms, antibiotics, adhesia

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