Ç̲ÑÒ (Contents)
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C. (P.) |
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Ðåäàêö³éíà ðàäà (Editorial Board) |
1 |
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Çì³ñò (Contents) |
2-4 |
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ÎÃËßÄÈ (REVIEWS) |
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| ÇÀÃÀËÜÍÀ ÕÀÐÀÊÒÅÐÈÑÒÈÊÀ ÄÈÔÒÅвÉÍÎÃÎ ² ÏÐÀÂÖÅÂÎÃÎ ÒÎÊÑÈͲ ÒÀ ¯Õ ÀÍÒÈÃÅÍÍÈÕ ÄÅÐÈÂÀҲ Êàë³í³÷åíêî Ñ.Â., Áàáè÷ ª.Ì., Ðèæêîâà Ò.À., Ñêëÿð Í.²., Ðÿáîâ³ë Î.Â., Ïëóãàòîð Ò.Ì., Àíòóøåâà Ò.². GENERAL
CHARACTERISTIC OF DIPHTHERIA AND TETANUS
TOXINS AND THEIR ANTIGENIC DERIVATIVES The modern views on diphtheria
and tetanus exotoxins’ structure are presented in this article. Some
aspects of toxoids obtaining by formaldehyde treatment are described.
Chemical modifications of above mentioned toxins that result loss of
toxicity but preservation of immunogenicity are characterized.
The stages of immunological preparations based on toxoids production
are pointed. It is showed that the most critical step of these vaccines
production is exotoxins inactivation because the new reaction products
after incubation with formaldehyde may cause negative influence on
vaccinated people.
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5-8 |
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| ղ̲×ÍÀ ÌÎÄÈÔ²ÊÀÖ²ß ÂÈÑÎÊÎÌÎËÅÊÓËßÐÍÈÕ Ë²ÊÀÐÑÜÊÈÕ ÇÀÑÎÁ²Â Êàøïóð Í.Â., Ìàðòèíîâ À.Â., Âîëÿíñüêèé À.Þ., Ïåðåìîò Ñ.Ä., Ñì³ëÿíñüêà Ì.Â. CHEMICAL MODIFICATION OF HIGH MOLECULAR MEDICATIONS Kashpur N.V., Martinov A.V., Volyanskiy A.Yu., Peremot S.D., Smelyanskaya M.V. A
biotechnology in the developed countries is on the stage of stormy
development, that conditioned by perspective of biotechnological
medications due to extremely low effective doses of these facilities.
Modern medicine knows the whole arsenal of medications on the basis of
the chemically modified biopolimers (Pegintron, L-asparaginaza,
adenozindeaminaza, Interferon - al'fa2b), which are widely utillized as
medical and prophylactic preparations at different somatic and
infectious diseases. Much looked over and monographs allow in a certain
measure to estimate the accumulated literary information both about
directions and about the biological, physiology and pharmacological
action of the chemically modified connections of different origin. It
is possible to draw a conclusion on the basis of analysis of literary
data, that chemical modification is a beautiful prospect for creation
of various medicinal preparations with the controlled and directed
action.
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9-21 |
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| ÑÓ×ÀÑͲ ϲÄÕÎÄÈ ÄÎ ÐÎÇÐÎÁÊÈ ÂÀÊÖÈÍÈ ÏÐÎÒÈ ÒÓÁÅÐÊÓËÜÎÇÓ ªëèñåºâà ².Â., Áàáè÷ ª.Ì., Æäàìàðîâà Ë.À., Êîëïàê Ñ.À., Áîáèðºâà ².Â. UP-TO-DATE APPROACHES TO TUBERCULOSIS VACCINE DEVELOPMENT Yelyseyeva I.V., Babych Ye.M., Zhdamarova L.A., Kolpak S.A., Bobireva I.V. Novel
concepts in development of vaccines for tuberculosis consist in
exchange BCG for more effective vaccine with long-term protective
efficacy or in development of vaccine for acquired immunity increase
and adult protection support. There are above 200 variants of vaccine
at the laboratory test stage in recent years, among of which there are
whole-cell (attenuated, vector), subunit, DNA-vaccines et al. Referred
to as the late booster strategy is the caveat that in highly
tuberculosis endemic regions such a vaccine will in many cases be given
to already sensitized to mycobacterial antigens persons (whether by
prior BCG vaccination, exposure to environmental mycobacteria, or
latent tuberculosis infection). Extensive use of novel vaccines for
tuberculosis is expecting not earlier than 2015 year.
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22-32 |
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| ÅÊÑÏÅÐÈÌÅÍÒÀËÜͲ
ÐÎÁÎÒÈ (EXPERIMENTAL STUDY) |
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| ÀÊÒÈÂͲÑÒÜ ÖÈÒÎÊ²Í²Â Ó ÕÂÎÐÈÕ ÍÀ ÃÎÑÒÐÈÉ ÊÎÐÎÍÀÐÍÈÉ ÑÈÍÄÐÎÌ Ïåðåìîò Ñ.Ä., Ñì³ëÿíñüêà Ì.Â., Âîëÿíñüêèé À.Þ., Êàøïóð Í.Â., Ïåðåìîò ß.Î. ACTIVITY OF CYTOKINES AT PATIENTS BY WITH ACUTE CORONAL SYNDROME Peremot S.D., Smilyanska M.V., Volyanskiy À.Y., Êàshpur N.V., Peremot Y. O. In the
article the levels of cytokines of patients are with a acute coronary
syndromes (AÑS) in combination with persisting herpetic infection
presented, endogenous mechanismes of compensation of immunological
disorders conducted for an estimation. It is set as a result of complex
research of immunocompetent cells, that a persisting herpes viruses
infection is associated with hyper by expression of proinflammatory
cytokines, initiating intravascular inflammation the same. It promotes
in same queue the risk of trombotic complications and can be considered
as an unfavorable sign of flow of AÑS. The flow of stable stenocardie
is characterized by the high indexes of anti-inflammatory cytokines:
IL-4, IL-10 at the unchanged values IL-1, IL-6, TNF-α. Destabilization
of ischemic heart disease is accompanied the increase of levels of
proinflammatory cytokines and decline of levels of anti-inflammatory.
The most meaningful changes of cytokines type are determined at a acute
coronale syndrome, combination’s with persisting of herpes viruses
antigens in immunocellulars.
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| ÏÅÐÅÕÐÅÑͲ ÐÅÀÊÖ²¯ ÏÐÈ ²ÌÓÍÎIJÀÃÍÎÑÒÈÖ² ÀÍÀÏËÀÇÌÎÇÍί ²ÍÔÅÊÖ²¯ ÌÅÒÎÄÎÌ ÐÍ²Ô Ïîõèë Ñ.²., Òèì÷åíêî Î.Ì., ˳ñíÿê Þ.Â., ×èãèðèíñüêà Í.À.,Êîñòèðÿ ².À., Êðóãëîâà Ò.À., Êèëèïêî Ë.Â., Ñåìåðåíñüêà ª.². CROSS-REACTIONS IN THE IMMUNODIAGNOSTICS OF ANAPLASMOSIS BY THE RIIF METHOD Pokhil S.I., Timchenko O.M., Lisnyk Yu.V., Chigirinska N.À., Kostirya ².À., Kruglova Ò.À., Kilipko L.V., Semerenska E.². The
immunological methods are the most often used for laboratory
diagnostics of anaplasmosis. There has been studied the possibility of
anaplasmosis antigen and rabbit anti-anaplasmic γ-globulins, contained
in experimental RIIF-test-systems, to enter into immunological
cross-reactions with correspondingly antigens of and antibodies against
microorganisms, which are philogenetically close to Anaplasmagenus, and
causative agents of other bacterial tick infections: Rickettsia
prowazekii, R. sibirica, Bartonella henselae, B quintana, Borrelia
afzelii, B. garinii, Brucella abortus, Francisella tularensis,
Coxiella burnetii. Under certain conditions, the anaplasmic antigen can
enter into immunological reactions with antibodies against R.
prowazekii, R. sibirica, B. henselae, B. abortus, F. tularensis, and
anti-anaplasmic γ-globulins can enter into immunological reactions with
F. tularensis antigen. However, the use of anaplasmic antigen and
anti-anaplasmic γ-globulins provides rather good quality level of
laboratory diagnostics of anaplasmosis if RIIF is carried out in
accordance with manufacturer’s instructions.
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38-44 |
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| ÂÈÂ×ÅÍÍß ×ÓÒËÈÂÎÑÒ² ÇÁÓÄÍÈʲ ÁÀÐÒÎÍÅËÜÎÇÓ ÄÎ ÀÍÒÈÁÀÊÒÅвÉÍÈÕ ÏÐÅÏÀÐÀҲ Áîíäàðåíêî À.Â. STUDY OF BARTONELLOSIS AGENTS SENSITIVENESS TO ANTIBACTERIAL DRUGS Bondarenko A.V. The
sensitiveness to antibacterial drugs of regional strains of Bartonella
was studied. Typical and regional strains of Bartonella spp. had a high
sensitiveness in vitro to most of the drugs: penicillins,
aminopenicillins, carboxypenicillins, cephalosporins (except of
cefotaximum), aminoglycosides, carbopenems, fluoroquinolones,
glycopeptides, rifampicinum, nitrobenzenes. 14-57% strains of
Bartonella spp. were resistant to macrolides (erythromycin,
clarithromycin, azithromycin) and tetracycline, which are traditionally
used at the treatment of bartonellosis.
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45-48 |
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| ÂÈÐÓÑÛ ÃÐÓÏÏÛ ÃÅÐÏÅÑÀ ÊÀÊ ÂÎÇÌÎÆÍÛÉ ÝÒÈÎËÎÃÈ×ÅÑÊÈÉ ÔÀÊÒÎÐ ÂÎÇÍÈÊÍÎÂÅÍÈß È ÐÀÇÂÈÒÈß ÈÍÔÅÊÖÈÎÍÍÛÕ ÌÈÎÊÀÐÄÈÒΠÑìåëÿíñêàÿ Ì.Â., Ïåðåìîò Ñ.Ä., Ìàòâèé÷óê Í.Â., Ïåðåìîò ß.À., Âîëÿíñêèé À.Þ., Ìàðòûíîâ À.Â., Êàøïóð Í.Â. HERPESVIRUSES AS POSSIBLE ETIOLOGIC FACTOR OF ORIGIN AND DEVELOPMENT OF INFECTIOUS MYOCARDITISES Smelyanskaya M.V., Peremot S.D., Matvichuk N.V., Peremot Y.A., Volyanskiy A.Y., Martinov A.V., Kashpur N.V. At the
virology inspection of patients myocarditis is expose high specific
gravity (86,4%) of antigens of herpesviridae in blood. At patients
combination of Ag of different types of herpesviridae ( in 74 % cases
of exposure) prevails myocarditis, while for the practically healthy
people of control group – a monoinfection detection more frequent. At
myocarditises in imunocytis of blood of patients in 61 % cases the
antigens of 3-4 different types of herpesviridae are revealed. More
frequent than all it HSV-1, CMV, EBV and HHV-6. Findings show a
necessity obligatory inspection of patients with a diagnosis
myocarditis on the herpesviruses.
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49-53 |
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| ÂÈÇÍÀ×ÅÍÍß ËÅÖÈÒ²ÍÀÇÍί ÀÊÒÈÂÍÎÑÒ² ̲ÊÐÎÎÐÃÀͲÇ̲ ÐÎÄÓ PROTEUS Þð÷åíêî Ë.À. DETERMINATION OF LECITHINASE ACTIVITY OF PROTEUS Yurchenko L.A. A research
purpose was a study of features of Proteus pathogeny festering -
inflammatory infections and role in them exciter. Lecithinase activity
was shown by 44.44% cultures of P.vulgaris and 91.66% cultures of
P.mirabilis. Nutrient medium was developed highly sensitive and
suitable for the simultaneous discovery of lecithinase activity and
suppression of swarming. The use of this nutrient medium in
microbiological practice will allow to promote quality of researches.
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54-57 |
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| ÕÀÐÀÊÒÅÐÈÑÒÈÊÀ ÏÀÒÎÃÅÍÍÈÕ ÂËÀÑÒÈÂÎÑÒÅÉ ËÀÁÎÐÀÒÎÐÍÈÕ ØÒÀ̲ CHLAMYDIA TRACHOMATIS ÏÐÈ ÅÊÑÏÅÐÈÌÅÍÒÀËÜÍÎÌÓ ²ÍÔ²ÊÓÂÀÍͲ ÒÂÀÐÈÍ Ãîí÷àðåíêî Â.Â. ÑHARACTERISTIC OF PATHOGENIC PROPERTIES OF LABORATORY STRAINS CHLAMYDIA TRACHOMATIS IN EXPERIMENTAL INFECTION WITH ANIMALS Goncharenko V.V. The
pathogenicity study results of the strains Chlamydia trachomatis on the
small laboratory animals are shown. The pathogenical features of each
strain is determined by the experiment in vivo. These strains
C.trachomatis is established to have the capability for the initiation
of inflammation processes and cause the definite pathomorphological,
pathophysiological and clinical changes with the animals. The
pathologic changes of the tissues were revealed by the histological
examinations in the organs of dead mice. Etiological role of these
strains for the diseases and following death of the animals confirms by
the laboratory testing (PCR, EIA, microscopy analyses). The
pathogenical features of the strains Ku (urogenital isolate) and Ar1-Z
(articular isolate) are weakvirulent and of the strains Ar2-Ê
(articular isolate) – high- virulent.
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58-66 |
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| ÎÖ²ÍÊÀ вÂÍß ÄÅßÊÈÕ ÖÈÒÎʲͲ ÏÐÈ ÏÍÅÂÌÎͲ¯ вÇÍÎÌÀͲÒÍί ÅÒ²ÎËÎò¯  ÅÊÑÏÅÐÈÌÅÍÒ² Êîëÿäà Ò.²., Áðóñí³ê Ñ.Â., Ìèõàéëè÷åíêî Ì.Ñ., Àòò³êîâ Â.ª.,Íåñòåðåíêî À.Ì., Êîëÿäà Î.Ì. ASSESSMENT OF THE LEVEL OF SOME CYTOKINES IN PNEUMONIA OF DIFFERENT ETIOLOGY IN THE EXPERIMENT Kolyada T.I., Brusnik S.V., Mikhailichenko M. S.,Attikov V.E., Nesterenko A.M., Kolyada O. N. Cytokines
have a key role in inflammatory response development. The cytokine
synthesis level has distinct features depending on the type of the
pathogen that induces the inflammatory process in the lungs. For
inflammatory responce, induce by Staphylococcus, a significant increase
in the levels of proinflammatory cytokines IFN-γ, IL-4 and IL-8 is
observed. IFN-γ, IL-4 and IL-8 in bronchoalveolar lavage. Candida
infection of the lungs is characterized by a dominating increase in
IFN-γ. In the case of combined inoculation of the two mentioned above
pathogens a decrease in IFN-γ and synthesis is observed. While
observing the dynamics, in case of Staphylococcal infection it is
established that the increased levels of proinflammatory cytokines are
gradually decreasing to normal values, as for the Candida infection a
significant decrease in IL-8 level, an activator of macrophages and
neutrophils, was established.
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67-71 |
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| ÂÏËÈÂ
ÑÓ̲ز ÎÌÅÃÀ-3 ÏÎ˲ÍÅÍÀÑÈ×ÅÍÈÕ ÆÈÐÍÈÕ ÊÈÑËÎÒ ÒÀ ÌÎËÅÊÓËßÐÍÎÃÎ ÊÎÌÏËÅÊÑÓ
ÐÍÊ-ÒÈËÎÐÎÍ ÍÀ ÔÎÐÌÓÂÀÍÍß ÏÐÎÒÈÒÓÁÅÐÊÓËÜÎÇÍÎÃÎ ²ÌÓͲÒÅÒÓ Ó ÒÂÀÐÈÍ Ç
Ò-Ê˲ÒÈÍÍÈÌ ²ÌÓÍÎÄÅÔ²ÖÈÒÎÌ ²ëü¿íñüêà ².Ô. THE INFLUENCE OF AN OMEGA - 3 POLY-NON-SATURATED FATTY ACIDS MIXTURE AND RNATILORIN MOLECULAR COMPLEX ON FORMATION OF ANTI-TUBERCULOSIS IMMUNITY IN ANIMAL WITH Ò-CELLULAR IMMUNE DEFICIENCY Ilyinskaya I.F. Àn absence
of adoptive anti-tuberculosis immune formation and expressive
macrophage dysfunction were demonstrated in animal with premorbidal
Ò-cellular immune deficiency. They stipulated an incompetence of
antimycobacteria drugs. It was found the possibility of macrophages
stimulation by the using of an omega - 3 polynon-saturated fatty acids
mixture and a molecular complex RNA-tiloron. That was resulted in
essential increase of treatment efficiency: after course of
immunocorrection indexes of MBT lesions of internals in animal were
decreased accordingly on 30 and 50 %, the number of died animal was
reduced accordingly in 2 and 8 times and the duration of their life was
prolonged accordingly at 50,0 and 77,9 %.
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72-81 |
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