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C.
(P.)
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Editorial Board
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1
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Contents
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2-6
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Review
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LABORATORY ASPECTS AND CLINICAL
SIGNIFICANCE OF BONE TURNOVER MARKERS
Yehudina Ye.D., Golovach
I.Yu.
Introduction. With an aging population, there is
a marked increase in prevalence of metabolic bone diseases, especially
osteoporosis. A serious complication of osteoporosis is non-traumatic
bone
fractures, which significantly impair quality of life and are
associated with
comorbid conditions and high mortality. Diseases associated with
impaired bone
remodeling require timely diagnosis, treatment and monitoring. The consequent public health and
socioeconomic burden warrant timely diagnosis, treatment and follow-up
of these
disorders. Knowing the limitations of radiological techniques,
biochemical
markers of bone turnover measurements come handy since the changes in
their
levels readily reflect bone physiology. Material
and methods. This paper presents a literature review concerning
bone turnover biomarkers with the aim of providing
comprehensive information on the applicability of these biomarkers for
clinical
use. A literature search was conducted in the PubMed, MedLine, Scopus
and
Embase databases from 1987–2019. Keywords used for search: bone turnover, bone formation, bone resorption, bone
biomarkers,
biochemical markers of bone turnover. We aimed to determine the
clinical
effectiveness, test accuracy, reliability, reproducibility and
cost-effectiveness of bone turnover markers for monitoring the response
to
osteoporosis treatment, predicting bone loss and fracture risk,
diagnostic of
osteoporosis, Paget’s disease, renal osteodystrophy, and certain
oncological conditions and rheumatic diseases. Results and
discussion. Bone turnover markers are a series of
protein or protein derivative biomarkers released during bone
remodeling by
osteoblasts or osteoclasts. Bone
biomarkers typically analyzed in high throughput automated routine
laboratories
are collagen degradation products, reflecting osteoclast activity and
collagenous or non-collagenous proteins produced by the osteoblasts.
All these
markers can be quantitated well from blood samples, serum being the
preferred
sample of choice. Although assays for urine examination were developed
for
quite a few markers, blood sampling generally detours the pre-analytic
issues
usually involving urine sampling. The most commonly used bone
resorption and
bone formation markers are discussed in this article. Biochemical markers of bone resorption are mainly
different fragments of
type I collagen, as well as non-collagen proteins that enter the
circulation
from the bone matrix resorption zone. The main biochemical indicators
used in
clinical practice as a criterion for bone tissue resorption are
pyridinoline,
deoxypyridinoline, tartrate-resistant acid phosphatase, and degradation
products of type I collagen - C- and
N-terminal telopeptide. All the afore mentioned markers have since been
superseded by the more sensitive and specific telopeptides of type I
collagen,
namely the C-terminal telopeptide (CTx). As compared to the bone
resorption
biomarkers, there is a larger repertoire of biomarkers of bone
formation,
reflecting osteoblast activity, namely serum bone-specific alkaline
phosphatase, osteocalcin and procollagen type I N-terminal propeptide
(PINP).
Although produced by the osteoblasts, osteocalcin may be defined as a
bone
turnover marker reflecting both bone formation and bone resorption,
since it is
also released from the bone matrix during bone resorption. PINP is more
extensively described in literature are compared to the other bone
formation
biomarkers. Conclusion. Biochemical
markers of bone turnover reflect bone homeostasis, i.e., the activity
of osteoblasts
and osteoclasts in both physiological and pathophysiological
conditions.
Although quite sensitive to a multitude of exogenous and endogenous
pre-analytical factors, bone markers are best used in monitoring
anti-osteoporosis therapy efficacy and compliance. Combination of bone mineral density measurement by dual
energy x-ray absorptiometry with
biochemical markers of bone turnover levels, at least one bone
resorption and
one bone formation marker, may potentially improve early detection of
individuals
at increased risk for bone loss and eventually non-traumatic bone
fracture.
Furthermore, they have widespread clinical utility in osteoporosis,
renal
osteodystrophy, certain oncological conditions and rheumatic diseases.
Key words:
diagnostics, bone metabolism, bone formation, bone resorption,
biomarkers of
bone tissue, biochemical markers of bone metabolism.
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7-18
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Experimental works
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BIOLOGICAL PROPERTIES OF CLINICAL STRAINS OF ESCHERICHIA COLI FROM DIFFERENT BIOTOPES
Voyda J.V., Birukova S.V.
Introduction. The results of laboratory diagnostics based on a limited
number of
phenotypic tests do not always allow to estimate with confidence the
etiological significance of Escherichia (even if their serological
belonging to
a certain group or biovar is taken into account). The accumulated data
indicate
the need for an in-depth study of the intraspecific diversity of E.
coli. The
aim of the work is to study phenotypic properties (morphological,
cultural,
biochemical, hemolytic activity) and genotypic characteristics
(evaluation of
plasmid prevalence and determination of their molecular size) of
clinical
isolates of E. coli from various biotopes, and to establish the
prevalence of
clinically significant multidrug-resistant strains among them. Material and methods The morphological,
cultural, biochemical properties, hemolytic activity of 677 clinical
strains of
E. coli isolated from different biotopes have been investigated. The material has been
sown on the 5% blood agar for accounting of the hemolytic forms. The
sensitivity
of E. coli to the
antibacterial drugs has been performed by disco-diffusion method
Keurby-Bauer usingstandard commercial discs on medium Mueller-Hinton.
The study
of the plasmid spectrum has been carried out using the alkaline method. Results and discussion. Coliform isolates with
hemolytic phenotype colonize the intestines of persons with dysbiosis 4
times
more often than in the control group (hemolitic active representatives
of the
control group have been found in (8,2±1,5) % of cases - according to accepted standards, the
percentage that is
allowed). Strains with hemolytic activity have been present in all
groups of
extracellular localization. Most often, this feature has been among the
strains
that were removed from the urine with complicated urinary system
infection
((66,6±7,5) %). The antibiotic susceptibility of
esherichias with hemolytic phenotype has been studied. A high prevalence of multidrug-resistant strains with hemolytic properties has
been established, both among representatives of normal intestinal
microflora
(49,2±9,8) %, and among uropathogenic serotypes (87,2±5,4) %. All
strains that
have been removed from the respiratory tract and areas of the skin and
soft
tissues were characterized by multiple resistance to the studied
antibiotics,
which is typical for hospital strains. Plasmid profile of 35 clinical
strains
of E. coli has been studied and
plasmids have been
found in 88.6% of Escherichia strains from different biotopes, most of
the
isolated strains were multiplasmid. The strains had from 1 to 8
plasmids with
sizes from 1 to 24 kb. It often has been found plasmid with size 19,3 among the studied multidrug-resistant
escherichias. Conclusion. It has been set that the isolates of Е. coli with hemolytic phenotype meet in all analyzed groups, frequency
of exposure depends on the
presence of festering-inflammatory process. The high sensitiveness to imipenem (96,6±1,5
% sensitive strains), gatifloxacin (92,4±2,2
%)
and
amikacin (80,0±3,3
%) has been found out, as well as growth of resistance to the
third
generation
cephalosporins (due to production of β-lactamases), other fluoroquinolones
and circulation of generous
amount mildly resistant strains to the derivatives of nitrofurans and
nitroxolinum, which occupy a leading place in the charts of the
protracted
empiric therapy of patients with chronic pyelonephritis.
Plasmid
profiles are quite individual strain characteristic, which determines
one of the
phenotypic characteristics of the pathogen. The study of plasmid DNA isolated from
clinical strains will make possible to determine with greater certainty
the
multidrug-resistant strains that
cause nosocomial infections and to identify ways of spreading hospital
infection in a particular hospital.
Key words: Escherichia coli,
biotope, biochemical properties,
hemolytic
activity, antibiotics, antibiotic resistance,
plasmid profile.
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19-25
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ANTI-MEASLES IMMUNITY ASSESSMENT IN UKRAINIAN HEALTH
WORKERS AND SCHOOL
TEACHERS
Volyanskiy A. Y., Kuchma I.Y., Kuchma M.V., Klisa A.A., Stepnova Yu. B.
Introduction Аdults suffer from measles harder than
children and more frequently have complications. Medics and school teachers are at increased
risk of measles disease. In the
case of measles, medics and teachers can infect a large number of
people in
contact with them with possible serious consequences. The study of
anti-measles
humoral immunity in doctors and teachers of Ukraine during the measles
epidemic
in the country is to identify and vaccinate susceptible individuals. Materials and
methods In
February-March 2019, the levels of specific anti-measles IgG in blood serum of 981 medics and 308
teachers aged 18 to 85 were
analyzed. The concentration of anti-measles
IgG was determined by ELISA using Ridascreen enzyme-linked
immunosorbent assays
produced by r-Biopharm (Germany) using the Lisa Scan EM enzyme-linked
immunosorbent analyzer (Czech Republic). In accordance with the
instructions
measles IgG levels of 150 mIU/ml or less were considered a negative
result,
from 151 to 200 mIU/ml inclusive - a dubious result, above 200 mIU/ml -
a
positive result. Statistical processing of the obtained data was
carried out
using non-parametric statistics methods with the Atte
Stat 12.0.5 statistical software package integrated into Microsoft
Excel 2013.
The average values of IgG concentration by age groups are equal to
sample
medians, since sample distributions do not fit the normal distribution
criteria
according to Kolmogorov-Smirnov confidence criterion estimates. Results and discussion. It was shown that the largest number of
measles-susceptible medics and teachers was observed in the age group
of 18 to
27 years (7.5% did not have a protective level of specific anti-measles
IgG,
and 5.6% showed a dubious level of protection). In the age groups from
28 to 37
years old and from 38 to 47 years old, a rather large number of
measles-susceptible individuals were also detected (5.3% and 4.6% with
no
protective level of anti-measles IgG and 5.0% and 3.7 % - with a
dubious level
of protection, respectively). The minimum number of medics and teachers
unprotected from measles was determined in the group aged 48 to 57
years (0.6%
of negative and 0.3% of doubtful results), and among the group over 57
years of
age, there were no individuals with a lack of protective levels of
antibodies
to measles virus. The possible reasons for the predominance of teachers
and
health workers unprotected from measles in the age groups from 18 to 47
years
and the absence of unprotected people over 57 years of age are
discussed. Conclusion The authors of the article concludes
that there is no need for vaccination against measles in medics and
teachers
over 57 years of age and recommends vaccination of representatives of
other age
groups only after determining of the anti-measles immunity level, since
more
than 90% of the tested population have protective IgG levels.
Key
words: vaccination, measles, specific anti-measles IgG, health
workers, school teachers
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26-29
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DYNAMICS OF IMMUNE
STATUS INDICATORS IN PATIENTS WITH RELAPSING-REMITTING MULTIPLE
SCLEROSIS
BEFORE AND AFTER TREATMENT OF BETFER-1A DRUG
Vdovichenko N.I.,
Kolyada T. І., Tupotilov O. V., Negreba T. V., Kolyada O. M.
The article presents the results of examination of 58
patients with relapsing-remitting multiple sclerosis (RRMS) treated
IFN-β.
Depending on the effectiveness of treatment, patients were divided into
two
groups: responders, included 37 persons (64%), in this group patients did not relapse during the year
from the beginning of treatment, the degree of EDSS remained unchanged
or
decreased, the group EDSS before the start of therapy was 2.2 ± 1.4
points.
After therapy, EDSS was 2.1 ± 1.4 points.- non-responders, this group
included
21 individuals, the patient was
included in the group if he had at least one relapse during the year
and / or
an increase in the degree of disability on the EDSS was found to be 1
point or
more. The group EDSS
before the start of therapy was 2.5 ± 1.5 points. After therapy, EDSS was 3.0 ± 1.5 points. The
immunological characteristics of the patients are presented, depending
on the
clinical efficacy of the treatment; the presence of disease-associated polymorphic
variants of HLA-DR has been determined. After the course of therapy
with IFN-β,
in patients with RRMS, regardless of the clinical efficacy of
treatment, the
relative number of T-lymphocytes and natural killer cells remained
significantly reduced. In the group of responders levels of circulating
immune
complexes and IgG levels did not differ from control after treatment,
in
contrast to the levels of lymphocytotoxic autoantibodies and complement
activity, which remained elevated relative to the control. After
treatment in
patients with low efficacy of IFN-β therapy (nonresponders), the
relative
number of CD19+ cells, as well as the levels of IgG, circulating immune
complexes and lymphocytotoxic autoantibodies remained elevated relative
to the
control group. The presence of the haplotype HLA-DRB1*1501-DQB1*0602 (one of its specific markers is allele G SNP rs9271366) is established to determine the association between
the disease-associated polymorphism of HLA-DR and the effectiveness of
IFN-β
therapy. The presence of
the minor disease-associated G allele was determined in 11 responders
(29.7%)
(heterozygous), another 26 patients (70.3%) were homozygous for major
allele A.
In the group of non-responders, 9 persons (42, 9%) were heterozygous,
12
(57.1%) were homozygous for major allele A.
Keywords: multiple sclerosis, IFN-β,
immune status, disease-associated
gene polymorphism
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30-34
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IMBALANCE OF IgG SUBCLASSES IN PRESCHOOL CHILDREN WITH
A DIFFERENT TYPE OF VACCINE RESPONSE TO MMR
Smilianska M.
Introduction. The only way to protect children
against measles, mups and rubella viruses (MMR) is vaccination.
However, among
vaccinated individuals there are always people who react poorly to
vaccine
preparations until they are completely insensitive due to their
individual
genetic or phenotypic characteristics. It is not always possible to
achieve a
full protective effect of vaccination. A comprehensive study of humoral
immunity factors, such as total IgG, their subclasses, virus-specific
antibodies, can predict the nature of the formation of a specific
immune
response to a vaccine, it is important when choosing a tactic for
vaccination
of preschool children. The aim of the work was to determine the
subclasses of
IgG (1, 2, 3, 4), their relationship and relationship with the type of
vaccine
immune response to the MMR vaccine in children of preschool age. Material
& methods. The content of serum IgG and subclasses was studied
in
children 6–7 years old with no specific response or a very strong
vaccine
response to one or more components of the MMR vaccine and it was 23%
ARI, or 32
children and 31% HRI, or 44 children, respectively. The control group
consisted
of 19 children of the appropriate age without herpes virus load and
with a
normal vaccination response to the MMR. Antibodies
of the IgG class to the measles virus were determined using an enzyme
immunoassay test system manufactured by IBL international GMBH - Meales
virus
IgG ELISA (Germany); rubella virus was determined using the enzyme
immunoassay
test system Rubella IgG-ELISA manufactured by Xema Co.Ltd. (Kiev,
Ukraine); to
the mumps virus was determined using an enzyme immunoassay test system
manufactured by R-Biopharm AC (Germany) - RADASCREEN® Mumps Virus IgG
(K5521).
To determine the content in the serum of IgG subclasses (IgG1, IgG2,
IgG3,
IgG4), ELISA was used using the IgG subclasses test systems
manufactured by
Vector Best. The determination of the antigens of the Herpesviridae
family was
carried out by an immunofluorescence method using specific monoclonal
mouse
antibodies of Santa Cruz Biotechnologu, Inc. (USA). Results.
An imbalance of IgG subclasses in preschool children with a different
type of
specific response to the CCP vaccine has been established. Thus, in the
group
with hyperreactive response, the level of IgG4 was significantly
elevated, and
in the group of reactive children, an increased level of IgG1 and a
decrease in
IgG3 were determined. The imbalance of IgG subclasses in preschool
children can
be associated with herpes viral load and the presence of individual
members of
the Herpesvirus family, which together leads to the development of a
different
type of immune response to the CCP vaccine. The determination of total
IgG and
subclasses, specific antibodies, can allow to predict the nature of the
formation of a specific immune response to the vaccine, it is important
when
choosing the tactics of vaccination of children of preschool age. Conclusion.
Quantitative determination of serum IgG in parallel with other classes
of
immunoglobulins is one of the mandatory tests in the study of the human
immune
status. Namely, an IgG imbalance of one or several subclasses can cause
a
partial imbalance in the system of regulation of the humoral immune
response in
children of preschool age (for example, a decrease in the level of
cytokine
production, or specific antibodies).
Keywords: gamma-glubulins, immunoglobulins, imbalance,
vaccination
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35-38
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BURNET ROOTS EXTRACT DRY: STUDY OF PHARMACO-TECHNOLOGICAL AND ANTIBACTERIAL PROPERTIES
Shulga L. I., Bezkrovna K. S., Soldatov
D. P., Osolodchenko T. Р.
Introduction. The basis for timely
development of new drug products, in particular the herbal ones, for
therapy of
the digestive system diseases is an increase in the total number of
gastroenterological patients. The surveys of the domestic
pharmaceutical market
as for the offer of the herbal drug products used for treatment of
various
diseases of the digestive system have found a limited range of the
drugs based
on the substances obtained from medicinal herbs and expediency of
search for
promising herbal sources. Based on the experience of the traditional
medicine,
Greater Burnet, the herbal raw material of which is used for production
of the
extract dry, has been selected for further examination. Whereas Burnet roots extract dry is anticipated to underlie the production of the
solid dosage forms, examination of its technological properties is
justified. The
scientific sources portray that the subterraneous organs of Greater
Burnet exert the bactericidal effect against microorganisms of
intestinal,
dysentery and paratyphoid groups, as well as antifungal activity of the
tincture of Burnet against 10 archival and clinical strains of Candida fungi,
which is a reason for microbiological screening of the obtained
extract. The object of this paper
is to study the technological parameters of Burnet roots
extract dry and to specify its antibacterial effect. Materials and methods. The study object is Burnet roots extract dry. The following methods
are used: optical microscopy method for specification of
crystallographic
characteristics, weight method upon surveying of the extract's moisture
absorption. The pharmaco-technological properties (fluidity, bulk
density,
tapped density, natural slope angle) have been studied; compressibility parameters and Gausner coefficient have been
calculated under the methods of the State Pharmacopoeia of Ukraine. The
antibacterial properties have been examined at the facilities of the
Biochemistry and Biotechnology Laboratory of the State Institution
"Mechnikov Institute of Microbiology and Immunology of NAMS of
Ukraine" through the method of diffusion in agar in the "wells"
modification on the reference testing cultures of microorganisms: P. vulgaris ATCC 4636, B. subtilis
ATCC 6633, S. aureus ATCC 25923, P. aeruginosa
ATCC 27853, E. coli ATCC 25922, C. albicans ATCC 885-653 with the
use of the drug product
"Chlorophyllipt", alcohol solution 10 mg/ml ("Galychfarm"
JSC, "Kyivmedpreparat" JSC, Ukraine) as the comparative drug. The
optical density of the microbial suspension of the said microorganisms
has been
in line with the scale McFarland 0.5 units. The obtained data have been
processed statistically in Excel program. Results
& discussion. The found anisodiametric shape of the particles
of Burnet
extract can anticipate a reduction of fluidity. The values of the
extract's
loss on drying are 3.77±0.10 %. The results of determining
the moisture absorption of the extract samples under the conditions of
relative
air humidity of 75% and 100% prove its hygroscopicity, which, upon
creation of
a solid dosage form, requires adjustment through processing of the
modern
auxiliary substances with the moisture-absorbing properties. The technological
parameters of Burnet roots extract dry are characterized;
reasonableness of
selection of the modern auxiliary substances with the aim to improve
such
technological parameter as fluidity is proven in the paper. The natural
slope
angle makes 34.20±0.58 degrees, being within the satisfactory range
acceptance
for the production. The values of Gausner coefficient and compressibility degree show a good fluidity of the extract, however they are not peculiar to its properties. Sensitivity of all the examined testing strains of
microorganisms to Burnet extract, including P. vulgaris
and P. aeruginosa, is proven; at the same time the comparative drug: "Chlorophyllipt" has shown no
antimicrobial effect against them. Conclusions. The pharmaceutical and technological properties
of
Burnet roots extract dry have been examined; subject to the results our emphasis is given
to a thorough approach for selection of auxiliary substances in
development of
the tablet composition based on it. The antibacterial effect of Burnet extract
in respect to the testing strains of microorganisms is determined and
microbiological researches as for specification of a potential antihelicobacter effect are scheduled.
Key words: Greater Burnet, extract dry,
pharmaco-technological properties, antibacterial properties,
gastroenterology
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39-43
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SYNTHESIS OF DYNAMIC RIBOFLAVIN DERIVATIVES AND THE STUDY
OF THEIR
ABILITY TO UREASE PHOTOINACTIVATION
Martynov A.V., Bomko T.V., Farber B.S., Nosalskaya T.N.,
Kleyn I.
Introduction. In one patents demonstrated the riboflavin ability to
inactivate
vaccine strains of viruses and bacteria, as well as cancer cells during
the
production of vaccines . DNA and RNA within living organisms have
specific
aptamers or flavin places where Riboflavin and its derivatives are able
to
selectively join . Upon further blue light irradiation 440-450 nm or
ultraviolet light at 280-350 nm such complexes between nucleic acids
and
riboflavin observed selective nucleolysis nucleic acids in the field of
riboflavin attachment .Research aim was to synthesize a series of
riboflavin
derivatives, including dynamic derivative having greater photodynamic
activity
(higher sensitivity to light) in the visible region spectrum at lower
concentrations.
Materials and methods. For gradient
HPLC analysis were used: acetonitrile and lithium perchlorate, perchloric acid in a kit for chromatograph
BD2003. For fixing the UV / spectrum of the synthesized compounds was
used
spectrophotometer Gene-quant - 1300. To analyze riboflavin
succinyl-dynamic
systems was used HPLC- system Milichrome A-02. To study the ability of
riboflavin derivatives for urease’s photo-inactivation reagents were
used:
test-system Urease-U and spectrophotometer Gene-quant – 1300 for fixing
end of
reaction. In addition, we used a set of
chromatography conditions for analysis of riboflavin derivatives:
gradient
separation of acetonitrile (from 0% to 100%) / 0.05 M lithium
perchlorate
buffer + 0.01 M perchloric acid at 40 0 C, and fraction
detection
in UV region. As a comparison, we used substance riboflavin (I). Results and discussion. The main
difference of (I) from dynamic riboflavin (IV) is much higher water
solubility
(up to 2%) for (IV). To compare the efficiency photoinactivation by
riboflavin
(I) and dynamic riboflavin (IV) was studied photoinactivation
efficiency of
urease as a model of microbial toxin and its transformation into a
toxoid. The
photoinactivators effectivity criterion is the ability to inactivate
urease by
disrupt performance of the active center of the enzyme. In our case -
to slow
down or completely block the urease ability to catalyze the hydrolysis
of urea.
Full succinylated derivative (V) acts in minimum effective
concentration 0,8 ± 0.1
µg/mL. Dynamic derivative (IV) with an immediate showed activity at a
concentration 0.2 ± 0.05 µg/ml and after
24 hours also not changed. The starting riboflavin (I) was very active,
regardless to storage time and they active concentration in solution
was 1.2 ±
0.2 µg/mL. The effective concentration statistically differed between
compounds
(I), (IV) and (V) at p≤ 0,05. Conclusion.
The first compound obtained
is combinatorial - maleylated / succinylated riboflavin (IV). The
synthesized
compound (IV) and (V) retain photodynamic activity. The minimum
effective
concentration of the compound (IV) with an immediate application
amounted to 0.2
± 0.05 µg/mL, whereas for the (V) this value was 0.8 ± 0.1 µg/mL.
Active
dynamic structure (IV) and even exceed the original riboflavin activity
at
immediate use after dissolution, it is still unstable and gradually
hydrolyzed.
The most stable activity possessed source riboflavin (I), its active
concentration is not dependent on the storage time of the aqueous
solution and
was 1.2 ± 0.2 µg/mL.
Keywords: riboflavin, photodynamic, vaccine, urease, dynamic
combinatorial
derivatives
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44-49
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STUDY OF THE INFLUENCE OF PHARMACEUTICAL FACTORS ON THE
OPTIMIZATION OF
THE RELEASE OF BIOLOGICALLY ACTIVE SUBSTANCES UPON RECEIPT THE WATER
EXTRACTS
FROM GYNECOLOGICAL PLANT MEDICAL COLLECTION
Konovalenko I. S., Polovko N. P.
Introduction. Infusions are water extracts that do not require either
sophisticated equipment
or expensive or scarce extractants. From a biopharmaceutical point of
view,
aqueous extracts provide good availability of drug substances. Compared
with
individual medicinal substances, they have a milder and simultaneous
complex
effect on the body. These circumstances are one of the reasons that
these
dosage forms, which appeared even before Galen, still retained their
significance. In the formulation of pharmacies, infusions and
decoctions can be
prepared independently, and can also be part of potions. Therefore, the
study
of water extraction technology is important for the practical
activities of the
pharmacist. Materials and methods. As
objects of the study a medical plant
collection was used, which included clover inflorescence, yarrow grass,
linden
flowers, thyme grass, which were selected for the pharmaceutical
development of
the collection for non-hormonal therapy of menopausal syndrome. Purified water was used as
extractant. The completeness of extraction was determined by the
quantitative content
of extractives and flavonoids content, expressed as rutin.
In order to study the effect of various
pharmaceutical factors on the release of extractives from medicinal
plant
materials, three collection fractions were studied that were identical
in composition
but different in degree of grinding components, which were obtained by
sifting
through sieves No. 1, 2, 3. Medicinal raw materials were ground by
grass
cutter. The particle size of the first fraction was 1–3 mm, the second
fraction
was 3–4 mm, and the third was 4–6 mm. Based on the obtained results, a
fraction
of 1–3 mm was selected for further studies to substantiate the
extraction
parameters Results & Discussion. To
optimize the extraction conditions, the
level of extractives from medicinal plant materials with a grinding
degree of
1–3 mm was studied under the above extraction conditions. As a result
of the
study, the influence of the degree of grinding of raw materials on the
level of
extractive substances obtained from aqueous extracts of plant
collection was
studied. It was
experimentally established that an increase in the time of infusion in
a water
bath from 5 to 15 minutes leads to an increase in the content of
extracts as
well as biologically active substances in the infusion. Further heating
in a
water bath does not increase the yield of extractives and flavonoids,
and a
color change may indicate the destruction of a number of biologically
active
substances under the influence of temperature. It is advisable to
increase the
infusion time before cooling for 30–45 minutes, since further cooling
does not
increase the content of active substances and leads to precipitation. Conclusion.
1. We studied the factors affecting
the optimization of the release of biologically active substances in
the
preparation of aqueous extracts from medicinal plant collection.2. We
investigated various extraction regimes of the drug collection and
found that
the optimal mode of extraction of biologically active substances from
the plant
collection is to insist on a water bath for 15 minutes, followed by
cooling at
room temperature for 30–45 minutes.
Keywords: water extraction, extractives,
medical plant material, gynecological drug.
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50-53
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PHYTOCHEMICAL SCREENING AND IN
VITRO ANTILISTERIAL ATTRIBUTES OF DIFFERENT AQUEOUS AND ETHANOLIC
LEAF
EXTRACTS
Daniel O. Ebakota, Onilude A. Abiodun, Obayagbona
Omoregbe Nosa
Introduction. Listeria monocytogenes
represents the Listeria species most commonly associated with
disease in
humans. The majority (99%) of the infections caused by L.
monocytogenes
are food-borne being ingestion of contaminated food especially
contaminated
ready-to-eat food products that do not undergo subsequent reheating.
This
organism has great economical implications in the food industry due to
recalls
of contaminated food products and temporary shutdown of many food
processing
plants. There has been lots of
interest recently in the role of complementary and alternative
medicines for
the treatment of various acute and chronic diseases. The revival of
interest in
the use of African medicinal plants by many developing countries and
the World
Health Organization (WHO) has led to intensified efforts to explore the
numerous plants with medicinal importance. A large number of
phytochemicals
belonging to several chemical classes have been shown to have
inhibitory
effects on all types of microorganisms in
vitro. Knowledge of the chemical constituents of plants and their anti-Listeria ability is desirable because
such information will be value for synthesis of complex chemical
substances.
The aim of this work was to study the
phytochemical qualitative profiles and in
vitro antilisterial attributes of aqueous and ethanolic leaf extracts from several plants
against earlier documented
Ready To Eat (RTE) associated multi
antibiotic resistant L. monocytogenes strains. Materials and Methods. The examined aqueous and ethanolic leaf extracts were
derived from Psidium guajava (guava),
Zingiber
officinale (Ginger), Dacryodes edulis
(African pear), Citrus aurantifolia (Lime),
Funtumia elastica (silkrubber), Vernonia amygdalina (Bitter
leaf), Cassia alata and Moringa
oleifera (horseradish
tree). The respective L. monocytogenes strains
utilized were; LMSN 70, LMEW 94 and LMMP 104. Invitro assay
of aqeous and ethanol estracts of plants was assayed
by agar well diffusion assay. Results
and discussion. Alkaloids, saponins, steroids, terpenoids, cardiac
glycoside, reducing sugar, phenolics, resins, flavonoids, and tannins were detected in Z. officinale ethanolic
leaf extract whilst cardiac glycoside was
absent in ethanolic leaf extracts of D.
edulis, C. aurantifolia
and F.
elastica. Alkaloids,
saponins, steroids, terpenoids, cardiac glycoside, reducing sugar,
phenolics,
resins, flavonoids, and tannins were
detected in crude aqueous leaf extracts of Z.
officinale and M. oleifera.F. elastica
ethanolic leaf extract displayed the highest
antilisterial potential at the least concentration; 100mg/ml whilst
amongst the
aqueous extracts, V. amygdalina
leaf extract exhibited maximal
antilisterial activities comparable with
antilisterial inhibitory growth zones elicited by the
respective ethanolic extracts
with the exception of M. oleifera extracts. Conclusion.
Further studies
aimed at the fractionation of the respective crude
extracts especially F. elastica
and exposing the respective
multi antibiotic resistant food borne L. monocytogenes strains
to these
fractionated leaf extracts should be
conducted.
Keywords: Antilisterial,
Agar well diffusion, Aqueous extract, Ethanolic extract,
Phytochemicals, RTE borne L. monocytogenes
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54-60 |
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SYNTHESIS AND
CHARACTERIZATIONS OF ABSORBENT DRESSING TURMERIC EXTRACT CURCUMIN
CHITOSAN-ALGINATE HYDROGEN AND ZNO NANO FOR MEDIATE AND HIGH EXUDATION
Astuti Amin, Wahyu
Hendrarti, Jenny Wunas
This study carried out the test of the effectiveness of
Hydrogel formula
of turmeric extract - chitosan and ZnO nano for wound in Oryctolagus
cuniculus, New Zealand rabbits, using Sodium alginate as a hydrogel
agent.
It was done to obtain a physically stable formula made from turmeric -
chitosan
and ZnO nano Hydrogel extracts to heal a wound. The stable hydrogels
were
tested further in vivo with three New Zealand rabbits and divided into
three
treatment groups, are stable Hydrogel preparations, positive controls,
and
negative controls. The results showed that stable hydrogel of chitosan
turmeric
extract and ZnO nano were effective to heal the wound as marked by a
reduction
in wound diameter faster than wound diameter in negative control
rabbits.
Further in the Anova test with a Complete Randomized Design (CRD) data
obtained
F count value of 596 > F table 1% 10.92. Indeed, there is a very
significant
difference between the three treatment groups. Additionally, the result
of
Tukey's HSD (honestly significant difference) test obtained that from
the three
treatment groups has a significantly different effect with negative
control and
not significantly different from positive control; preparations that
have been
known to have a healing effect on wound.
Keywords: Absorbent Dressing, Alginate, Chitosan, Curcumin,
ZnO Nano, Healing.
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61-69
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CHOICE OF THE PRESERVATIVE IN THE COMPOSITION OF VAGINAL GEL WITH
RESVERATROL AND HYALURONIC ACID FOR TREATMENT OF UROGENITAL SYMPTOMS IN THE
CLIMAХ PERIOD
Ivaniuk O.I., Strilets O.P., Yarnykh T.G.
Aim. Microbial
contamination of a product is possible in the process of production, storage
and use, which can shorten the shelf life and adversely affect the safety
profile of the drug. Therefore, an important criterion for evaluating the
quality of a medicinal product is microbiological purity. The aim of the study was to determine and compare the
antimicrobial effectiveness of preservatives in the composition of the vaginal
gel with resveratrol and hyaluronic acid. Materials and methods. The objects of the study were samples of vaginal gel with various
antimicrobial preservatives, which have no irritant effect on the mucous
membranes, and are safe for the human body. Preservative selection studies have
been conducted using the antimicrobial preservative efficacy evaluation
technique described in SPU 2.0 (Vol. 1, Sec.5.1.3, p. 773). The principle of
the method is that to the samples of the finished dosage form with various
preservatives, which are in the primary packaging, introduce a certain number
of test microorganisms and store these samples at a certain temperature (from
20 to 25 ºC) in a dark place. Immediately after inoculation and at specified
intervals (2, 7, 14 and 28 days), samples (usually 1.0 g) are taken from the
inoculated samples and the number of viable microorganisms is determined. All
studies were performed in aseptic conditions, using a laminar box (Biological
Safety Cabinet AC2-4E1 " Esco", Indonesia). Staphylococcus aureus
ATCC 6538, Pseudomonas aeruginosa ATCC 9027, Candida albicans ATCC 885-653,
Aspergillus brasiliensis ATCC 16404 were used as test microorganisms for
inoculation of gel samples. Results. The research
on the choice of antimicrobial preservative in the composition of vaginal gel
for the treatment of urogenital symptoms, has been conducted, according to
which it can be concluded that samples of gels with preservatives Cosgard 0.5%,
Leucidal 2.0% and EuxylPE 9010 0.7% meet the criterion "A » According to
the requirements of SPU for non-sterile medicines and are promising for further
research on the development of composition and optimal technology of semisolid
dosage form of vaginal gel. According to the research, the antimicrobial
effectiveness of 0.5% Cosgard preservative gel was higher than the 2.0%
Leucidal preservative gel samples and 0.7% Euxyl PE 9010, which will contribute
to the quality of the developed gel during its storage. On the basis of the
conducted researches the expediency of preservative Cosgard at a concentration
of 0.5% introduction into the composition of vaginal gel with resveratrol and
hyaluronic acid has been established. Conclusions.
It has been established that the optimal antimicrobial preservative in the
composition of vaginal gel is the preservatives Cosgard in concentration 0,5%. The conducted studies are promising for
further research on the development of composition and optimal technology of
semisolid dosage forms.
Key words. Vaginal gel, antimicrobial
preservative, resveratrol, hyaluronic acid.
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70-74 |
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