Annals of Mechnikov's Institute 2011 4
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(REVIEWS)



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ֲ ϲ̲ ² ֲ Ҳ, 23-25 2011 ., . ʲ ABSTRACTS XV
Congress of microbiologists, epidemiologists, parasitologists Ukraine "Problems and Evolution epidemic process and parasitic systems Infections Leading the Present" on 23-25 November 2011, Kharkov



6-240

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INFLUENCE OF ANTHROPOGENIC ELECTROMAGNETIC FIELDS ON THE ADAPTIVE MYCOBACTERIUMS POLYMORPHISM
Vlasenko V.V., Vlasenko I.G.,  Volyanskiy A. Yu.
In this article the features of development of exciter of tuberculosis are lighted at the action of the electromagnetic field of antropogenic origin subject to the condition in vitro. It is shown that the microstructure of pathogenic of tuberculosis after treatment an ionizing and unionizing irradiation is not violated, and reproductive activity grows, that is confirmed the results of cultural researches and electronic and computer microscopy.
 
241-245

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TO  BE  OR  NOT TO  BE A PATHOGENS:  MICROBIOCENOSIS  OF  UPPER  RESPIRATORY TRACT, PHYSIOLOGICAL INFLAMMATION AND PATTERN RECOGNITION RECEPTORS
Kamyshniy O. M., Voitovich O. V, Topol I. O., Degen A. S.
This review own data is dedicated to ability of macroorganism to adequate response to the invasion of various microorganisms. The traditional idea of indigenous or normal microflora, as a simple symbiotic populations of microorganisms from different biotopes of the human past decade has shattered and does not reflect its real biological significance. However, the microflora is extremely sensitive to adverse factors and changes its parameters can be indicated by such influences. The main role in maintaining tolerance and initiate the immune response is the innate immune factors. Determination of antigen is based on the recognition of evolutionary highly conserved structures, called microorganism-associated molecular images. In this review present several hypotheses lower-level alarm on pattern-recognition receptors as a result of their interaction with the microorganism-associated molecular images of commensal microflora. 

246-251

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BIOCOMPATIBLE CALCIUM-PHOSPHATE COATINGS FOR METALIC IMPLANTS
Sukhodub L.B., Volansky A.Yu., Sukhodub L.F., Guschilik B.I., Gayduchok I.G., Kazmirchuk V.V., Schulga N.M., Povolokina I.V., Parusov A.V.
State and perspective of the creation and investigation of biocompatible calcium-phosphate coatings on metalic implants have been discussed in the overview. The methods for obtaining these coatings, such as plasma sputtering, biomimetic methods, electroforetic method, ion sputtering, thermodeposition with a cooling system and others were considered. Some perspective as for broadening of an action field of the coatings for implants via incorporation into calcium-phosphate nanostructured surface some specific bioactive molecules and drugs also are discucced. 


252-257

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SOME PECULIARITIES OF CLINICAL PICTURES AND WAYS OF IMPROVEMENT OF TREATMENT OF INTESTINAL INFECTIONS AT CHILDREN TODAY.
Olkhovska O.N.
The main clinical peculiarities of Shigellosis, Salmonellosis, Escherichiosis at 420 yearly age children were performed.  Its  characterized  by  hight  percent  of  gastroenterocolitic  forms,  vomiting,  ketoacidosis,  and dehydratation on presentation. The clinical values of ultrasonic investigations of haemodynamic indexes were investigated. It was proposed to add ultrasonic haemodynamic indexes to standards of treatment of severe form of intestinal infectious diseases at children.
 

258-261

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ANTIMICROBIAL ACTIVITY OF ECOLOGICALLY SAFE SOLUTION OF NANO SILVER OBTAINED BY CHEMICAL METHOD
Mikhienkova A.I., Nikonova N.A., Romanenko L.I.
In experimental investigation the antimicrobial activity of silver nano particles (8-12 nm) in colloid solution and in the matrix of high-dispersive silicon dioxide stabilized by the mixture of polyvinylpyrrolidone and sodium dodecyl sulphate was studied. The quantity of the latter one was decreased to the level of limited permissible concentration. In result of conducted work it was proved that obtained solution kept stability and the high antimicrobial effect for test strains of E. coli and S. aureus: after 1-2 hours of exposition the reduction of them for more than 5 lg was reached. The obtained data allowed recommend studied solutions in the base of silver nano particles for the use as ecologically safe preparations with antimicrobial activity in the different fields of medicine, biology and food industry.
 

262-265

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Anticomplementary  activity  of  COLON  microbicenosis  REPRESENTATIVES  IN  PATIENTS  WITH RHEUMATOID ARTHRITIS
Kuchmak O.B., Klymnyuk S.I.
The classic bacteriological method was used for investigation of colon microbiocenosis in 72 patients with rheumatoid arthritis at the age of 20 to 55 years. It was revealed that patients with rheumatoid arthritis had dysbacteriosis mainly I (37,5%) and II (41,7%) degree. Some populations of bacteria isolated from these patients  had  anticomplementary  activity  that  was  most  prominent  in  gram-negative  than  in  gram-positive microorganisms. Lactobacillus rhamnosus GG probiotic strain had a pronounced antagonistic activity against important representatives of colon microbiocenosis: E. coli, Enterococcus spp., Klebsiella spp., S. aureus, but more than 60,0 % of clinical strains of Pseudomonas aeruginosa were insensitive to the action of this antagonist.

266-270

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CYTOKINES AND C-REACTIVE PROTEIN CONTENT IN SERUM BLOOD OF PATIENTS WITH CHRONIC LARYNGITIS DISEASE
GarjukG. I., KulikovaE.A., ZaetirSamir
Some kinds of interleikines and C-reactive protein of acute phase of patients with chronic laryngitis disease were investigated. There is cytokines explosion of the patients chronic laryngitis with persistent herpes simplex virus. Comparative investigation cytokine profile in serum blood demonstrated: balanced reaction cytokines profile of patients chronic laryngitis without persistent herpes simplex virus and dysbalanced reaction of patients laryngitis (hyperergation). Increased content IL-6 and low content γ-interferon and tumor necrosis factor (TNF-α) are predisposition of chronisation inflammation processes in larynges. This situation needed in sighting correction.

271-277

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STUDY OF INFECTION OF PATIENTS WITH BENIGN PROSTATIC HYPERPLASIA, COMPLICATIONS AND UNCOMPLICATED ACUTE URINARY RETENTION
Rymsha O. V.
The paper presents the results of bacteriological studies of pathological material from patients after surgery for BHPG. complications and no complications of acute urinary retention. Material for the study were - urine intraoperatively removed tissue of the prostate and prostate secretion. Clinical isolates of microorganisms were characterized by morphological, Cultural and biochemical properties. The greatest number of complications and longest postoperative period were observed in patients with chronic infection caused by gram flora. Frequency of Gram-negative bacteria in the samples was 69.3%, gram-positive - 25%.

278-280

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IN THE STUDY OF MICROBIOCENOSIS OF SKIN AT CREATION OF EXPERIMENTAL MODEL OF PHLEGMON FOR RATS.
Skakovskaya O.O., Stepansky D.O., Kremenchutsky G.N., Drozdov A.L.
The positive role of microorganisms of skin is well-known in maintenance of health of macroorganism.  A question about the role of microorganisms of skin in development of phlegmon remains to the not end studied. Understanding of conformities to law of change of microbal landscape of skin at a festering-inflammatory defeat (to the phlegmon) will allow considerably to optimize diagnostics and treatment of this pathology.  In this work the  changes of microbal  landscape of  skin of  intact  rats were appraised  and at the design  of  phlegmon. Heterogeneity of specific composition of microflora of intact skin of white rats is rotined, taking part in an experiment, which is presented saprophyte and conditional-pathogenic microorganisms.  Specific composition of skin of rats changed during the design of phlegmon, new kinds appeared ( S. aureus, K. pneumoniae). In 7 % cases from the place of phlegmon bacterial associations were selected, presented  S. aureus, K. pneumoniae and fungi.

281-283

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FEATURES OF BACTERIAL BIOFILMS FORMING ON THE SURFACE OF CHOLESTETOMAS AT CHRONIC SUPPURATIVE OTITIS MEDIA
Mogylivska N.M., Voytsehovskiy V.G., Soushko Yu O.,  Borysenko O.M., Srebnyak I.A.
The results of detection of microorganisms on the surface of cholesteatomas and also from the exsudate of cavity of middle ear are presented in this work. By the method of raster electronic microscopy on the surface of cholesteatomas which removed during operations, had been found bacterial biofilms, and through bacteriological researches were isolated microorganisms of different species (S. pidermidis, P.  eruginosa, S. aureus, S. saprophyticus, E. coli, E. cloacae, Micrococcus spp., Citrobacter spp., Corynebacterium spp.). Comparison of bacteriological and electronic microscopic data has confirmed a hypothesis that biofilms are not found by standard cultural methods. It is necessary to carry out bacteriological studies of cholesteatomas, removed during operations, for the purpose of improvement diagnostics and prescription etiotropic antibiotic therapy.

284-288

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FEATURES  OF  INTOXICATION  SYNDROME  IN  PATIENTS  WITH  ACUTELY  PROGRESSIVE
PULMONARY TUBERCULOSIS
Lebid L.V., Kireyev I.V., Poteyko P.I., Liashenko A.A.
The features of intoxication syndrome in patients with acutely progressive pulmonary tuberculosis during the epidemic  of  tuberculosis  in  Ukraine.  The  analysis  is  performed  on  two  groups  of  patients  with  acutely progressive pulmonary tuberculosis: 115 patients treated in a tuberculosis clinic in 1993-1995 and 100 patients treated in 2006-2009. For objective assessment of the severity of intoxication syndrome was used test available -haematological toxicity index, which was calculated for the clinical analysis of blood. It is established that patients, treated in 2006-2009, had a slower regression of intoxication syndrome. Studying the dynamics of the haematological toxicity index in the dynamics after three and six months of chemotherapy led to the conclusion about the need for intensive detoxification, not only in patients with severe intoxication syndrome, but also for weakly expressed.
289-292

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PROMISING USE OF ANTIVIRAL AGENTS IN THE TREATMENT OF PATIENTS WITH ACUTE CORONARY SYNDROME AND PERSISTENT HERPES VIRUS INFECTION
Yakovleva L., Peremot J., Peremot S., Smelyanskaya M.,  Kashpur N., Volyanskiy A., Alois Bomba, Dankovich A.
Under the influence of specific antiviral immunoglobulin treatment reduced the level of CRP was 2.4-fold, TNF-α by 2.3-fold, and IF leukocytes by 24%.Dynamics of lipoprotein parameters in patients with ACS at high risk of coronary events, after the application of specific imunoglobulinoterapii shows a decline in CA and 1.5 times tended to normalize the levels of LDL-C and HDL-C.Stabilization of coronary heart disease in a subgroup of patients treated with anti-immunoglobulin, nearly one and a half times higher than patients with GCS group who received  only conventional  basic therapy,  accompanied by clinical  deterioration in 13.3%  of  patients  and resulted in 2 cases of fatal coronary events.

293-297

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Iί VISCUM ALBUM
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IZOPRENOIDS COMPOSITION OF THE VISCUM ALBUM RAW MATERIAL
Upyr D.V., Kyslychenco B.S., Martynov A.V.
Qualitative and quantitative composition of terpenoids in the  Viscum album L. raw material, harvested from different trees of the host (linden and poplar), has been studied by gas chromatography method. The content of terpenoids in grass mistletoe was 0.28% and 0.14% respectively. It was determined 133 compounds are compounds were identified. Among the identified compounds 35 were found in both extrakts, 9 - only in mistletoe  harvested  from lime  (1.8-cineole,  camphor,  β-evdesmol,  etc.), 19  substances -  only in mistletoe harvested from poplar (α-isophorone, β-farnesene, β-ionone, farnesol, α-bisabolol etc).

298-302

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DEPENDENCE OF CLINICAL MANIFESTATION OF ROTAVIRAL MONO- AND MIXED INFECTION AT CHILDREN FROM INITIAL LEVEL OF INTERLEUKINS
Kirsanova T.A.
The article presents the results of the study of definition the cytokine status at children with rotaviral and rotaviral-bacterial infections and communication revealing between indicators of level interleukins and clinical manifestation of disease. Reliable direct connection between the level of interleukins and clinical manifestation of disease has been detected. It appears to be the factor which regulates inflammatory process, and causes the formation of pathological process in the gastrointestinal tract and the systemic inflammatory answer. That in the whole defines the presence and full-blown character of the clinical displays of the disease.

303-306

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PHOTODYNAMIC INFLUENCE ON PLANKTON FORMS AND BIOFILMS OF S. PYOGENES, S. AUREUS AND PROTEUS SPP. ISOLATES
Tsyganenko A.J., Mishina .M., Dubovik E.S., Mishin Y.M.
The effect of blue and red light diode radiation was studied on the plankton forms and biofilms of bacteria, causative agents of purulent inflammatory processes, depending on chronoinfrastructure. It was revealed that isolates reacted differently on the light diode radiation: the blue light mainly inhibited the ability of biolofilm production by plankton cells and the ability of preformed biofilms to produce plankton cells, while the red light in most cases did not influence the formation of primary biofilm and inhibited the release of plankton cells by the preformed biofim to a less extent.

307-311

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PREPARATIONS  ON  BASIS  OF  PHENIC  HYDROPHILIC  SUBSTANCE  OF  PROPOLIS AND  THEIR APPLICATION ARE IN THERAPY OF INFECTIOUS DISEASES.
Radchenko E.A., Shulga N.N., Gushilik B.I., Al'khuseyn Mustafa Ali, Chernyaev S.A.
The problem of search of effective antibacterial preparations is conditioned high morbidity and wide prevalence of infectious diseases. In spite of intensive skrining, conducted in the whole world, the amount of antibacterial preparations at the row of infections is limited. We are study antibacterial and antivirus activity of terapevticheskikh preparations on the basis of phenic hydrophilic substance of propolis and perspective of their use is rotined for treatment of infectious diseases. Study clinical efficacy "Propoltin" and Propomedin. Which are created on the basis of phenic hydrophilic faction of propolis and the prospect of their application is rotined in treatment of infectious diseases.

312-313

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HLAMYDIA TRACHOMATIS, , -
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GENOTYPING OF HLAMYDIA TRACHOMATIS OBTAINED FROM PATIENTS WITH UROGENITAL PATHOLOGY IN NORTH-WEST REGION OF UKRAINE
Lytovchenko O.
The genotype distribution of Chlamydia trachomatisin patients with urogenital pathology in North-West region of Ukraine was investigated with aid of restriction analysis. The most prevalent genotypes were E, G and F, detected in 24 %, 23,5% 21,2 % of cases.

314-315

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THE INFLUENCE OF PHYSICAL AND PHYSICOCHEMICAL FACTORS ON IMMUNOBIOLOGICAL PROPERTIES OF DIPHTHERIA TOXIN
Kalinichenko S.V., Babych, E.M., Kivva F.V., Ryabovol E.V., Gorbunov L.V., Ryzhkova T.A., Sklyar N.I.,  Kovalenko O.I., Katrych V.A., Zhdamarova L.A., Bobyreva I.V., Kalinichenko E.O.
The influence of physical (ultrasound, temperature) and physicochemical (formalin, temperature, ultrasound) factors  on  the  immunobiological  properties  of  diphtheria  toxin  and  its  modifications  was  studied.  It  was established that exposure of these physical factors did not lead to the destruction and inactivation of the native diphtheria toxin. Formalin volume fraction reduction by half did not significantly affect the specific activity and safety of diphtheria toxin. Ultrasonication of the samples with formalin resulted in reduction of epidermal reaction up to the total absence of any signs of hyperemia. It was determined that certain diphtheria toxin derivatives, obtained by physical and chemical factors, are nonhazardous.
316-320

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