Ç̲ÑÒ (Contents)
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C. (P.) |
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Ðåäàêö³éíà ðàäà (Editorial Board) |
1 |
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Çì³ñò (Contents) |
2-7 |
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ÎÃËßÄÈ (REVIEWS) |
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ÏÐÈÍÖÈÏÈ ÑÒÂÎÐÅÍÍß, ÌÅÕÀͲÇÌ Ä²¯ ÒÀ Ê˲Ͳ×ÍÅ ÇÀÑÒÎÑÓÂÀÍÍß
ÏÐÎÁ²ÎÒÈʲ (ÎÃËßÄ) CREATION
PRINCIPLES, MECHANISM OF ACTION AND CLINICAL APPLICATION OF
PROBIOTICS (Review) In the presented review the general data concerning probiotics is considered, i.e. definitions of the term, classification principles, and the core benefits of usage if compared to antibiotics. Are noted The criteria of choice and the characteristics of the main sorts of bacteria used as basic probiotics. Special attention in terms of usage for producing bacteriemic medicines is paid to Bacillus spore-former bacteria, as normal micro-flora exogenous components that do not produce biofilms. Bacillus spore-former bacteria are also able to produce a wide spectrum of biologically active substances, including antibiotics, lysozyme, proteolytic enzymes, and able to influence the immunological reactivity of macro-organism, therefore stimulating the growth of secretory immunoglobulins`, macrophages`, natural killers` activity. The Subalinum biological features are considered. The basic for Subalinum is genetically modified strain of Bacillus subtillis 2335/105 with a plasmid, containing the gene for alpha-2 human interferon. The characteristics for genetically modified strains of E. coli is given, as being perspective for creating probiotics for effective treatment of diarrhea, caused by enterotoxigenic E.coli and Vibrio cholera. They are proposed for creating recombinant strains of bifidobacteria for atherosclerosis and cardiovascular diseases` treatment and prevention. They are also prospective for making Lactococcus lactis recombinative strain for ulcerative colitis and Crohn's diseases` treatment. The potential dangers of drugs based on living organisms are being discussed. Some of the mechanisms of probiotics influence on the immune system and aspects of clinical application of probiotics for preventing and treating dysbiosis, atopy, intestinal infections of bacterial and viral, cardiovascular, cancer and secondary immunodeficiencies, are highlighted. The research paper contains the possibility of co-using probiotics as vaccination adjuvant. Keywords: bacteriemic preparations, probiotics, dysbacteriosis, gen-modified strains, Subalinum, Bacillus, probiotic therapy.
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8-16 |
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ÌÎËÅÊÓËßÐÍÛÅ
ÎÑÍÎÂÛ ÏÅÐÑÈÑÒÅÍÖÈÈ ÂÈÐÓÑÀ ÝÏØÒÅÉÍÀ-ÁÀÐÐ Â ÎÐÃÀÍÈÇÌÅ ×ÅËÎÂÅÊÀ THE
MOLECULAR MECHANISMS OF EPSTEIN-BARR VIRUS PERSISTENCE IN THE
HUMAN ORGANISM Key words: Epstein-Barr virus, persistence, virus lytic replication, phase of latency, chronic active EBV infection.
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17-26 |
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ÑÓ×ÀÑÍÈÉ
ÏÎÃËßÄ ÍÀ ÅÒ²ÎËÎÃ²Þ ² ˲ÊÓÂÀÍÍß ÒÀÇÎÂÎÃÎ ÁÎËÞ ÏÐÈ ÇÀÏÀËÅÍͲ
ÃÅͲÒÀË²É Ó ÌÎËÎÄÈÕ Æ²ÍÎÊ The
modern view of the etiology and treatment of pelvic pain in young
women with genitals inflammation Sensitive
issue of modern gynecology can be considered widespread and
substantial "rejuvenation" of inflammatory diseases of
the pelvic organs in women of reproductive age.Ascending path of
infection prevails in the pathogenesis of inflammatory diseases of
the internal genital organs. Invasion of microbes in the internal
genital organs may occur during the various manipulations,
different pelvic operations and in the postpartum period. The
degree of colonization of microorganisms of the vagina and cervix
plays an important role in the development of the infectious
process. In obstetrics and gynecology inflammatory diseases can be
caused by pathogenic and non- pathogenic (opportunistic)
microorganisms. Among the pathogens causing the defeat of the
female genital organs, most often found N. gonorrhea, C.
trachomatis, T. vaginalis. Opportunistic pathogens, part of the
normal flora of the genital tract, in certain circumstances, can
become agents of post-partum, post-abortion, post-operative
complications and inflammatory diseases of the female genital
organs. Among the opportunistic pathogens that are part of the
normal microflora of the female genital organs, found hemolytic
and non-hemolytic streptococci (the most important are
streptococci groups A, B, D), coagulase-negative staphylococci and
micrococci (allocated 60% and 35% of healthy women, respectively).
They can cause secondary infectious processes of the urinary
system, inflammatory diseases of the genital organs of pregnant
women and mothers with immunosuppression. These microorganisms are
often the agents of inflammatory diseases in the newborn,
especially with low weight and malnutrition children.
Gram-negative opportunistic bacteria that are isolated from the
genital tract, can also be agents of inflammatory processes of
various localization. Escherichia coli is the most frequently
obtain and cause urinary tract infection in pregnant and
postpartum women. It is also causative agent of postpartum sepsis
and postoperative peritonitis. Among other gram-negative bacteria
should be noted genus Klebsiella. Therefore, therapy of
inflammatory diseases of the pelvic organs high demands due to the
risk that represents this state to the reproductive function of
patients. The mixed nature of the infection, the increasing rates
of resistance of pathogens to antibiotics creates difficulties in
selecting antimicrobial therapy, which remains the empirical till
now. The inclusion of specific bacteriophages and plant
antiseptics in therapeutic regimens for local treatment of
inflammatory diseases of the vagina associated with an excessive
colonization of the vaginal mucosa by aerobic opportunistic
bacteria justified and can serve as an alternative to the
traditional antibiotic therapy. All this determines the necessity
for further studying the etiology and pathogenesis of infectious
and inflammatory complications, the development of new highly
effective methods of prevention and treatment.
Key words: inflammatory diseases, antimicrobial treatment, harmons, women reproductive system, dismenorrhea. |
27-31 |
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ÅÊÑÏÅÐÈÌÅÍÒÀËÜͲ
ÐÎÁÎÒÈ
(EXPERIMENTAL STUDY) |
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ANTIMICROBIAL
ACTIVITY OF [1,2,4]TRIAZOLO[4,3-à]PYRAZIN-8(7H)-ONE DERIVATIVES Today
the
problem
of
microbial
resistance to
antibacterial
agents
becomes
the global one.
Antimicrobial
drugs
that
are
in
the pharmaceutical market
do
not satisfy
the
needs of
modern
treatment
regimens,
particularly
Hospital-acquired
infections.
Therefore,
the
search for new
and
effective means
of
this pharmacological group
is
an important task
of
medical
chemistry.
From
the
literature
it
is known that
derivatives
of
[1,2,4]triazolo[4,3-a]pyrazine
show
a wide range of
biological
actions,
including
antimicrobial
and
fungicidal.
This
makes
it relevant
microbiological
study
of
primary
derivatives
of
[1,2,4]triazolo[4,3-a]pyrazine
for
identifying
promising
compounds
of
the series
and
then
study
it
in
biological
experiment.Using
the PASS
C&T
(Prediction
Activity
Spectra
for
Substances:
Complex
& Training)
program
and based
on
published
data, we have generated
virtual
library
of derivatives
of [1,2,4]triazolo[4,3-a]pyrazine.
As
a
result,
we
have
received
35
new
synthetic
compounds
of
7
series
that were not
previously
described
in the literature. Materials
and methods. The
research of
antimicrobial
and
fungicidal
activity
of
the synthesized
compounds
was
carried out
in
the laboratory
of antimicrobial
agents GA
"Mechnikov
Institute of microbiology and immunology"
under the leadership of
PhD,
senior scientist V.V.Kazmirchuka.
The
activity of
the
synthesized
compounds
were
studied by
conventional
method
of the two-fold
serial
dilutions
in
liquid
and
solid
nutrient
medium.
For
primary
screening
we
have used
a
set of
clinical
and
reference
strains
of microorganism:
Escherichia
coli
ATCC
25922 (F-50),
Staphylococcus
aureus
ATCC
25923 (F-49),
Bacillus
anthracoides
ATCC
1312, Pseudomonas
aeruginosa
ATCC
27853, Candida
albicans
ATCC
885-653. As
the
reference
preparations
were
chosen
Palin
-
modern
antimicrobial
agent
of class
of
fluoroquinolones,
Nevigramon
-
nalidixic
acid
derivative and
Fluconazole
-
modern
antifungal
agent.
Activity
of
substances
determined
by the
minimum
bacteriostatic
(MBstK)
and
minimal
bactericidal
(MBcK)
concentrations.
All
experiments
were
accompanied by
appropriate
controls.
Results
and
Discussion.
As
a result of
microbiological
screening
of
35
compounds
we have allowed
to
identify a number
of
derivatives of
[1,2,4]triazolo[4,3-a]pyrazine-8(7H)-one
with
antimicrobial
and
antifungal
activity.
The most
pronounced
effect
showed
compounds
that contains
in
their
structure
aryl
moiety
with
halogen
atom,
or
N-arylatsetamide
group
in
position 3
or
2
of the heterocycle.
Principal
condition
for
the
demonstration of
antifungal
activity
is
presence
of
Sulfur
atom
in
the
triazole
cycle.
Conclusions.
The
substance
of
the
series
N7-aryl/benzyl-3-thioxo-[1,2,4] triazolo[4,3-à]pyrazin-8(7H)-ones
showed
the
best
antimicrobial
and
antifungal
activity
of
all
synthesized
compounds.
Compound
7-(3-chloro-2-methyl-phenyl)- 3-thioxo-[1,2,4]triazolo[4,3-à]pyrazin-8(7Í)-one
4{4}
showed
high
values
of
antimicrobial
activity
against
gram-negative
microorganisms
(ÌBstÊ
– 12.5-25.0 mkg/ml,
ÌBcÊ
– 25.0-50.0 mkg/ml)
and
was
the
most
promising
for
further
development.
Key
words:
[1,2,4]triazolo[4,3-a]pyrazin-8(7H)-one
derivatives,
antimicrobial
activity,
antifungal
activity. |
32-38 |
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ʲËÜʲÑÍÅ
ÂÈÇÍÀ×ÅÍÍß ÎÑÍÎÂÍÈÕ
ÃÐÓÏ
ÐÅ×ÎÂÈÍ
Â
ÃÐÀÍÓËÀÕ
ÍÀ
ÎÑÍβ
ë³êàðñüêî¿
ðîñëèííî¿ ñèðîâèíè ÄËß
˲ÊÓÂÀÍÍß
ÇÀÕÂÎÐÞÂÀÍÜ
øëóíêîâî-êèøêîâîãî
ÒÐÀÊÒÓ QUANTITATIVE
DETERMINATION OF MAIN
GROUPS
OF
SUBSTANCES IN
GRANULES
ON
THE BASIS OF
MEDICINAL
VEGETABLE RAW MATERIAL
FOR
TREATING GASTROINTESTINAL
DISEASES In
the last time a
significant
increasing
of gastrointestinal
tract
diseases has been observed.
The poor
quality
and
irrational
feeding,
environmental
pollution,
psychological and
other factors is the
causes of this.
Very
often the gastrointestinal tract
has
a
multifactorial
pathological
effects,
also
affecting
the
hepatosphere
organs
and
urogenital
system.
Also
a
great importance
have accompanying
disorders
of the
central
nervous system. Thus we
must to require a
comprehensive
approach
to the creation
of
drugs
for
use in
gastroenterology,
the
assortment
range
of which
should
be expanded.
Advantageous
position
in
this case
takes
a
phytotherapy
using
drugs
based on
medicinal
plant raw material, which acting
on the
main
areas
of
the pathological
process.
For
this purpose
the scientists
from the National
University
of Pharmacy
(Kharkov,
Ukraine) was
created
a
complex
herbal
drug in
the
form of granules
under
the code name
"Poligerbagastrin",
includes
the
following types of
medicinal
plant raw material powders:
helichrysum
arenarium
flowers,
corn
stigmas,
horsetail
grass,
knotweed
grass,
horse
chestnut
seeds,
licorice
roots
and wheat
bran.
Materials
and methods. To
determine
the quantity of
biologically
active substances
the method
of
spectrophotometry
in
the visible and
UV
spectral region was
used.
This
method
is
well studied and available,
equipped
with
high-precision
hardware.
He
also
described
in
the Ukrainian
normative
documents
and contained
in the
world's
leading pharmacopoeias.
For determination was used the unifieds methods, which shown
in
pharmacopoeia
monographs
and
other reference
literature.
Determination
was
carried out
with
a spectrophotometer
HP
8543
UV-VIZ
of «Hewlett
Packard» company,
USA.
Results
and discussion. For
the quantitative determination
of
flavonoids
was
used
a
methodology, which based on
the
complexation reaction of
isolated
by
acid hydrolysis
and
extraction
with ethylacetate
hydrolysis
products
with
aluminum
chloride
in
methanol
-
ethyl
acetate
-
acetic
acid environment and calculation
of
flavonoid content
as
hyperoside recalculating.
For
the quantitative determination
of
polyphenols
was used
the methods of State Pharmacopoeia
of
Ukraine
"Determination
of
tannins
in
herbal
drugs"
or European
Pharmacopoeia «Determination of tannins in herbal drugs».
The
method is based
on
the
color
reaction
of
polyphenolic substances,
which
including
in the
plant
raw material,
with
phosphomolybdic-tungsten
reagent
and
measuring
the
optical
density
of
the resulting solution
at
the wavelength of
760
nm.
The content
of
total polyphenols
in
the drug
was
determined
as pyrogallol
recalculating.
Conclusions.
1.
The determination of quantitative
content of
biologically
active substances
in
granules
based
on
medicinal
plant raw material
for
the treatment of
gastrointestinal
tract
diseases is carried out.
2. In
the studied
sample
of
granules
contents
of
flavonoids,
calculated
as
hyperoside,
which equal to 0.43%
and
the
polyphenols,
calculated
as
pyrogallol,
which equal
to
0.44%
has
been established.
3.
The
data obtained can
be
used to develop
the normative
and technical documentation.
Keywords: gastrointestinal tract diseases, medicinal plants raw material, granules, spectrophotometry, quantitative determination.
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39-43 |
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ÈÑÑËÅÄÎÂÀÍÈÅ
ÂÊËÀÄÎÂ ÁÎÊÎÂÛÕ ÀÌÈÍÎÊÈÑËÎÒÍÛÕ ÎÑÒÀÒÊÎÂ
ÏÎËÈÌÈÊÑÈÍÀ B3
 ÅÃÎ ÑÂßÇÛÂÀÍÈÅ Ñ ËÈÏÎÏÎËÈÑÀÕÀÐÈÄÎÌ ÂÍÅØÅÉ ÌÅÌÁÐÀÍÛ E.COLI
ÌÅÒÎÄÀÌÈ ÌÎËÅÊÓËßÐÍÎÃÎ ÌÎÄÅËÈÐÎÂÀÍÈß MOLECULAR
MODELING STUDY OF THE CONTRIBUTIONS OF SIDE AMINO ACID RESIDUES OF
POLYMYXIN B3
TO ITS BINDING WITH E.COLI
OUTER MEMBRANE LIPOPOLYSACCHARIDE Introduction. Last decades, antimicrobial peptides (AMPs) are the subject of intense investigations aimed to develop effective drugs against extremely resistant nosocomial bacterial pathogens (especially Gram-negative bacteria). In particular, there has been greatly renewed interest to polymyxins, the representatives of AMPs which are specific and highly potent against Gram-negative bacteria, but have potential nephrotoxic side effect. A prerequisite of purposeful enhancement of therapeutic properties of polymyxins is a detailed knowledge of the molecular mechanisms of their interactions with cell targets. Lipopolysaccharide (LPS), the main component of the outer leaflet of outer membrane of gram-negative bacteria, is a primary cell target of polymyxins. The aim of the paper was to study the peculiarities of molecular interactions of polymyxin Â3 with lipopolysaccharide of the outer membrane of gram-negative bacterium. Materials and methods. The complexes of polymyxin Â3 (PmÂ3) and its alanine-derivatives with E. coli outer membrane lipopolysaccharide were built and studied by molecular modeling methods (minimization, simulated annealing, docking). Atom coordinates of polymyxin Â3 and LPS structures were taken from nuclear magnetic resonance and X-ray crystallography experiments, respectively. The AMBER03 force field was used with a 1.05 nm force cutoff. Longrange electrostatic interactions were treated by the Particle Mesh Ewald method. Results and discussion. Alanine scanning of PmÂ3 molecule has been carried out and the role of its side amino acid residues in the formation of complex with lipopolysaccharide has been investigated. It has been shown that substitutions of polymyxin’s Dab residues in positions 1, 3, 5, 8 and 9 for alanine markedly reduce the binding energy of PmB3-LPS complex, where as the similar substitutions of residues in positions 2, 6, 7 and 10 leave the binding energy virtually unchanged. Structural aspects of antimicrobial action of polymyxins have been analyzed. Changes of minimal inhibitory concentrations (MIC) of alanine-derivatives of polymyxin and binding energies in dependence on the alanine substituent position were parallel, except Ala2- PmÂ3 mutant. Conclusions. Polymyxin’s side Dab residues, especially Dab1, Dab5, Dab 8 and Dab9, essentially contribute to the energy of polymyxin Â3 binding with LPS of the outer membrane. Contribution of the others polymyxin’s side residues to the binding energy of the complex of polymyxin Â3 with LPS was insignificant. Key
words:
polymyxin,
lipopolysaccharide,
alanine
scanning,
binding energy |
44-54 |
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ÂÈÊÎÐÈÑÒÀÍÍß
ÊÎÌÏ'ÞÒÅÐÍί ÏÐÎÃÐÀÌÈ PASS
ÒÀ
ÌÎËÅÊÓËßÐÍÎÃÎ ÄÎʲÍÃÓ ÄËß ÏÎØÓÊÓ ÍÎÂÈÕ ÀÍÒÈÊÎÍÂÓËÜÑÀÍҲ THE
APPLICATION OF
PASS-COMPUTER
PROGRAMME AND MOLECULAR DOCKING FOR THE SEARCH OF NEW
ANTICONVULSANTS Introduction. Currently the priority goal of designing drugs is the integration of the methods of organic chemistry and pharmacology. The application of computer programmes which can predict interaction of potential drugs with molecules of biological targets makes possible to decrease the number of experiments on laboratory animals. Thereby the economic efficiency of production of new medicines increases. Models of the research the anticonvulsant activity (in particular, korazol, thiosemikarbazid, strychnine, etc.) are the most rigid experimental models of pharmacological screening, which basically entails the pains of laboratory animals or their death. The application of computer programmes in the research of potential anticonvulsants has economic and social desirability and high level of importance for the pharmaceutical science and health care. The most perspective methods of research are the virtual screening, molecular docking. These methods allow to evaluate the affinity of a substance to a specific biological target, i.e. to identify an inhibitor of a particular enzyme or protein. Material and methods. We have carried out the construction of 50 groups substances (507 hypothetical structures). We have chosen the five-membered di(three)azaheterocycle as basic pharmacophores to form virtual structures because firstly their structure is similar to cyclic conformation of neurotransmitter and secondly according to the literature perspective anticonvulsants had already found among these derivatives. Computer prediction of pharmacological activity for all compounds of virtual database was performed using the PASS (Prediction of Activity Spectra for Substances) computer programme. Results obtained by PASS-computer programme showed prospects of search the anticonvulsants among 10 groups of derivatives di(three)azaheterocycles (probable activity (Pa) of substances of these groups are from 0.5 to 0.84). In order to determine the potential anticonvulsant activity of 1,2,3(1,2,4)triazole, 1,3,4-oxa(thia)diazole we investigated the mechanisms of action that involve the interaction of the ligand NMDA-, GAMKA- or glutamate receptors and GABA-AT ligand-enzyme. We have perfomed docking research for our structures and for known anticonvulsants using the Fast Dock method, in which both protein and ligand are rigid (Software SCIGRESS; Fujitsu, Fukuoka, Japan). We have evaluated affinity of the investigated structures with molecules biotargets: GABAA receptor protein (PDB code 1GNU), glutamate receptor protein Glu-1 (PDB code 1EWK), GluN1 NMDA receptor protein (PDB code 3Q41) and protein enzyme GABA-AT (PDB code 1OHW). Results and discussion. As a result, we have obtained the values of scoring functions Consensus, which enabled to evaluate affinity compounds and biological anticonvulsant targets and identify 11 perspective groups of compounds (number of compounds 190) that can selectively inhibit NMDA, a GABA - or glutamate receptors and GABA aminotransferase enzyme in comparison with known anticonvulsant drugs. The number of active groups of the results PASS prediction according to the obtained results is 10 (the number of compounds 168). It should be noted that result of docking research coincided with the results of PASS prediction for eight groups of compounds. Conclusion . 1. Eleven groups of compounds derivatives of 1,2,3(1,2,4) -triazols, 1,3,4-oxadiazoles and 1,3,4- thiadiazoles was selected for further screening as perspective anticonvulsants; 2. GABA-ergic mode of action for 8 groups of derivatives and glutamatergic mode of action for 3 groups of derivatives five-membered di(three)azaheterocycle was predicted. Keywords:
docking,
anticonvulsant
activity,
PASS computer program, di (three) azaheterocycle. |
55-60 |
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ÏÐÎÒÈ̲ÊÐÎÁͲ,
Ô²ÇÈÊÎ-ղ̲×Ͳ ÂËÀÑÒÈÂÎÑÒ² ˲ÊÀÐÑÜÊÈÕ ÀÍÒÈÑÅÏÒÈ×ÍÈÕ ÏÐÅÏÀÐÀҲ ANTIMICROBIAL,
PHYSICAL AND CHEMICAL QUALITIES OF MEDICINAL ANTISEPTIC DRUGS In
our research results of the study of antimicrobial, physical and
chemical qualities of antiseptic medicines of decamethoxin (DCM).
Antimicrobial activity of DCM, palisan, decasan, deseptol against
srains of S.aureus
(n
56),
S.epidermidis (n
26),
E.coli (n
24),
P.mirabilis (n
11),
P.vulgaris (n
8) was studied by means of method of serial dilutions. Obtained
data of mass spectrometry study of antimicrobial compositions with
constant concentrations of DCM have shown that medicinal forms of
DCM are complex physical and chemical systems, because of
different origin and number of adjuvant ingredients used during
their fabrication. Among synthetic quaternary ammonium agents
there have been found the substance (commercial name of medicine
is decamethoxin) to have high antimicrobial activity against
strains of gram-positive and gram-negative microorganisms, an also
C.albicans.
There was found that antimicrobial activity of antiseptic palisan
had been higher comparably to DCM in equivalent concentration. The
composition and concentrations of acting agents and the
methodology of preparation of palisan have been substantiated on
the basis of microbiological, mass spectrometry characteristics of
antiseptics DCM, palisan.
Key
words:
antiseptics, decamethoxin, decasan, palisan, mass spectrometry. |
61-66 |
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ÑÊÐÈÍÈÍà ÀÍÒÈÌÈÊÐÎÁÍÛÕ ÑÂÎÉÑÒ ÑÏÈÐÒÎÂÎÄÍÛÕ ÂÛÒßÆÅÊ ÈÇ
ÍÅÊÎÒÎÐÛÕ ÂÈÄÎÂ ÐÀÑÒÈÒÅËÜÍÎÃÎ ÑÛÐÜß ÑÎÄÅÐÆÀÙÅÃÎ ÕÈÍÎÍÏÐÎÈÇÂÎÄÍÛÅ SCREENING
OF ANTIMICROBIAL PROPERTIES OF ETHANOLIC EXTRACTS FROM SOME KINDS
OF RAW MATERIALS WITH QUINONEderivatives Keywords:
antimicrobial
properties, raw material, extracts, hydroquinones, naphtoquinones,
anthraquinones. |
67-72 |
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ÌÅÄÈÖÈÍÀ (MEDICINE) |
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ÇÀÑÒÎÑÓÂÀÍÍß
ÏÐÅÏÀÐÀÒ²Â
ÍÀ
ÎÑÍβ
ÁÀÊÒÅвÀËÜÍÈÕ
˲ÇÀÒ²Â
Ó
ÒÅÐÀϲ¯
ÕÐÎͲ×ÍÎÃÎ
ÒÎÍÇÈ˲ÒÓ Ó
ÏÀÖ²ªÍҲ Ç
ÐÅÂÌÀÒίÄÍÈÌ ÀÐÒÐÈÒÎÌ THE
USE OF PREPARATIONS BASED ON BACTERIAL LYSATES IN THE TREATMENT OF
CHRONIC TONSILLITIS IN PATIENTS WITH RHEUMATOID ARTHRITIS In the therapy of decompensated form of chronic tonsillitis (CT) were used as immunomodulatory agents IRS and Ismigen. These bacterial lysates differ in the bacterial setting, the method of preparation (chemical, mechanical) and the method of application.Rheumatoid arthritis (RA) is one of the important factors that could significantly complicate the therapy of chronic tonsillitis. RA is a chronic immune inflammatory disease that progressively affects connective tissue mostly of the peripheral joints and it has a wide range of extra-articular manifestations. The aim of our study was to explore the dynamics of immunologic indicators during the active disease and treatments in patients with decompensate form of chronic tonsillitis, including tonsillitis complicated with RA. Materials and methods. 33 patients with decompensate form of chronic tonsillitis in active period of disease observed during the study. Patients were divided into the following groups: 24 persons with the decompensate form of CT, 9 persons with the rheumatoid arthritis and 9 persons with the decompensate form of CT complicated with RA in remission stage. The control group consisted of 15 apparently healthy persons. Concentrations of serum IgA, IgM, IgG were determined by the method of radial immune diffusion by Manchini. Levels of sIgA, IFN – γ and rheumatoid factor in the blood serum of patients were evaluated using ELISA test systems of "Vector-best". Patients of group CTD (with decompensate form of chronic tonsillitis) were divided into subgroups CTD1 and CTD2, depending on the applied treatment. Both subgroups treated with standard therapy for two weeks and received Derynat during 1 month by 2 drops in each nostril twice a day. After 30 days of the standard therapy beginning the subgroup CTD1 patients received IRS 19 during two weeks, one intranasal inhalation in each nostril 3 times a day. Patients subgroup CTD2 and CTD+RA instead IRS 19 received Ismigen after 30 days of the beginning of the standard therapy, during 10 days, sublingually 1 per day.Patients with RA were at the stage of clinical remission and do not receive basic disease-modifying drugs for at least 6 months after the last course of therapy. The effectiveness of the treatment was assessed by the general state of the patients according to oropharyngoscopy, concentration of IgA, IgM, IgG and IFN-γ in serum and sIgA in pharynx secret after 45 days of observation.Antibodies to microbial antigens (antistreptolysin O-hemolytic streptococci) were determined before treatment and after 45 days using passive hemagglutination reaction.Statistical analysis of the results was performed using the Mann-Whitney U test. According to the accepted level of reliability index value between the groups (p), which constituted or were less than 0.05. Results and discussion. 1. In all groups before treatment was determined deficiency of humoral immunity. The level of sIgA was significantly reduced relative to controls as in patients with exacerbation of decompensate form of chronic tonsillitis with RA (CTD + RA group) and in the group with chronic tonsillitis without RA (CTD group). The level of IgG before treatment group CTD + RA was significantly elevated relative CTD group, the comparison group (patients with RA without tonsillitis) and the control group.2. Preparations Ismigen and IRS 19 demonstrated high efficacy as immune modulatory agents in the treatment of decompensate form of chronic tonsillitis in the acute stage. Combined with Derynat use of these drugs after standard therapy allowed to normalize humoral immunity. In the subgroup of patients which received Ismigen level of sIgA after 45 days was significantly higher with respect to the subgroup that received IRS 19. These differences may be related both to the various quantitative and qualitative antigenic composition of the drug, and the difference in the method and circuit their application and in accordance with the bioavailability of the components of bacterial lysates.3. Ðatients at CTD + RA before treatment is characterized by elevated levels of ASO for the group CTD. ASO level was significantly higher than normal level in CTD and CTD + RA groups.4. Application of bacterial lysates containing antigens of hemolytic streptococci, such as Ismigen or IRS 19, does not cause increase level of ASO and rheumatoid factor in all groups of patients, indicating an insufficient level of immunogenicity of these drugs to influence the course of RA in the inactive phase of the disease. Keywords: immunity, chronic tonsillitis, rheumatoid arthritis, immunoglobulins, bacterial lyzates.
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73-77 |
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ÄÎÑ˲ÄÆÅÍÍß
ÏÐÎÒÈ̲ÊÐÎÁÍί ÀÊÒÈÂÍÎÑÒ²
ÊÎÌÁ²ÍÀÖ²É ÔÎÑÔÎ̲ÖÈÍÓ
Ç ÖÅÔÅϲÌÎÌ
ÒÀ ÔÎÑÔÎ̲ÖÈÍÓ
Ç Ò²ªÍÀÌÎÌ
ÙÎÄÎ
ÏÎ˲ÀÍÒÈÁ²ÎÒÈÊÎÐÅÇÈÑÒÅÍÒÍÈÕ
ØÒÀ̲ ÑÈÍÜÎÃͲÉÍί
ÏÀËÈ×ÊÈ
THE
STUDY OF
ANTIMICROBIAL
ACTIVITY OF
COMBINATIONS
OF FOSFOMYCIN
WITH
CEFEPIME
AND
FOSFOMYCIN
WITH
TIENAME
IN RESPECT
POLYANTIBIOTIC-RESISTANT
STRAINS OF
PSEUDOMONAS
AERUGINOSA The rapid decrease in sensitivity of pathogens septic infections to antimicrobial agents has led to significant difficulties in the fight against antibiotic-resistant infections even in developed countries. One solution to this problem is a method of combining antimicrobial different pharmacological groups. The most promising for combination drugs are derivatives of phosphonic acids, which are able to deeply penetrate biological film and microbial cells and enhance the antibacterial action of other antibiotics. A study of the effectiveness of combination antibiotic fosfomycin with cefepime and fosfomycin with tiename on polyantibiotic-resistant strains Pseudomonas aeruginosa. Set the antibiotic used in the experiments performed by P.aeruginosa strains of "serial dilutions" and the disco-diffusion method. The efficacy of combinations of antibiotics was carried out by determining the minimum inhibitory concentrations routine method in vitro method "checkerboard". The results of experimental studies the combination of cefepime on multiresistant strains Fosfomycin on Pseudomonas aeruginosa show a significant decrease in the MIC of cefepime in combination on ten of the thirteen strains. MIC Fosfomycin significantly decreased relative to the nine strains. In calculating the index FIX appears that a synergistic effect of the combination of antibiotics studied (FIX ≤ 0,5) was observed on 69.2% of the subjects strains of P.aeruginosa. In experiments on three strains observed effect summation antimicrobial antibiotics specified combinations (FIX> 0,5, ≤ 1,0), one strain - indifferent effect (FIX> 1.0). You can also combined fosfomycin with tiename significant (greater than 2-fold) reduction in MIC these antibiotics was observed only for 2 subjects cultures P. aeruginosa. Calculation of the FIX showed that combined use of fosfomycin with tiename created largely indifferent effect refers to the strains of P. aeruginosa. Thus, studies have shown a high degree of synergy combination of fosfomycin with cefepime on polyantibiotic-resistant strains strains of P. aeruginosa. Keywords: combinations of the antibiotics, polyantibiotic-resistant strains, checkerboard method. |
78-82 |
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²ÑÒÎÐ²ß ÌÅÄÈÖÈÍÈ |
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Ê 150-ËÅÒÈÞ ÑÎ
ÄÍß ÐÎÆÄÅÍÈß ÏÅÐÂÎÎÒÊÐÛÂÀÒÅËß ÂÈÐÓÑΠÄÌÈÒÐÈß ÈÎÑÈÔÎÂÈ×À
ÈÂÀÍÎÂÑÊÎÃÎ 150th
ANNIVERSARY OF VIRUSES DISCOVERER
DMITRI IOSIFOVICH IVANOVSKY The article is devoted to
the 150th anniversary of the birth day of Dmitri Iosifovich
Ivanovsky. On the example of tobacco mosaic disease, he first
proved the existence of a new type of pathogens – viruses. Dmitri
Ivanovsky is considered as founder of a new, independent science -
Virology.
Keywords: D.I.Ivanovsky, discoverer of viruses, tobacco mosaic virus disease, the founder of Virology.
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83-84 |
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ÏÀÌßÒÈ ÁÎÍÄÀÐÅÍÊÎ ÂÈÊÒÎÐÀ ÌÈÕÀÉËÎÂÈ×À (1940-2014) |
85-86 |
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²íôîðìàö³éí³ ëèñòè |
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ÒÀÊÒÈÊÀ ˲ÊÓÂÀÍÍß ÇÀÃÎÑÒÐÅÍÍß ÕÐÎͲ×Íί ÃÅÐÏÅѲÐÓÑÍί
²ÔÅÊÖ²¯ Ó Ä²ÒÅÉ, ßʲ ×ÀÑÒÎ ÕÂÎвÞÒÜ
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87-88 |
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ÂÈIJËÅÍÍß Ê˲Ͳ×ÍÎ ÇÍÀ×ÓÙÈÕ ÅÍÒÅÐÎÊÎʲÂ
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89 |
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×ÓÒËÈÂÎÑÒ² ÇÁÓÄÍÈʲ ÃͲÉÍÎ-ÇÀÏÀËÜÍÈÕ ÇÀÕÂÎÐÞÂÀÍÜ ÄÎ
ÁÅÒÀ-ËÀÊÒÀÌÍÈÕ ÀÍÒÈÁ²ÎÒÈʲ ÏÐÈÑÊÎÐÅÍÈÌ ÌÅÒÎÄÎÌ |
90 |
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