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C. (P.)
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Editorial
Board
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1
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Contents
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2-6
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Reviews
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Features of SARS-CoV-2 infection
and directions of drug and vaccine creation
Volyansky A.Yu., Davydova T.V., Kuchma M.V., Yudin IP,
Kuchma I.Yu.
Introduction: Until the beginning of the 21st century human
coronaviruses were known as the cause of typical seasonal àcute respiratory disease. Highly pathogenic coronaviruses were identified as the
cause of severe acute respiratory syndrome (SARS) with high mortality
in 2002 – SARS-CoV, and then in 2013 as the causative agents of Middle
East respiratory syndrome – MERS-CoV. They have caused severe
respiratory disease in humans because of their ability to adapt to the
host, increasing affinity for human airway receptors. The emergence a
new coronavirus in 2019 caused a rapid increase in the incidence of
severe acute respiratory syndrome and turned into a pandemic. Background: The new coronavirus SARS-CoV-2, which causes Covid-19
(coronavirus infection disease), was first detected in December 2019 in
Wuhan, the capital of Hubei province, from where it quickly spread to
China and continued to spread to Italy and other countries of Europe
and after around the world, the number of confirmed new cases is
increasing daily. Rationale: oronaviruses are members of the order Nidovirales of the
family Coronaviridae and divided into 4 genera: α- and β-coronavirus,
which infect only mammals, and γ- and δ-coronavirus, which mainly
affect birds. The central place is occupied by the genus β-coronavirus,
which includes especially dangerous pathogens of lethal human pneumonia
– SARS-CoV, MERS-CoV, SARS-CoV2. In turn, β-coronavirus divided into
four subgenres: A, B, C, D. SARS-CoV-2 is β-coronavirus, subgroup B,
Sarbecovirus. Coronaviruses affecting humans currently have 7 species.
They divide into 2 groups. 1st group has 4 common types of coronaviruses that
cause about 15% of typical seasonal SARS: HCoV-229E, NL63, OC43 and
HKU1. Usually these types cause mild forms of upper respiratory tract
diseases in children (and less often in adults), but in some cases
(like other SARS viruses) cause interstitial pneumonia (in newborns,
children with immunodeficiency, people with cancer, patients who take
immunosuppressive therapy, the elderly with heart failure and chronic
lung disease). 2nd group has 3 types of coronaviruses, which in a large
percentage of cases SARS. 1. SARS-CoV – the epidemic began in China in
November 2002 from live animal markets in Foshan (Guangdong Province),
spreading to Asia and the world. Considered, horseshoe bats can be the
natural reservoir of SARS-CoV. SARS-CoV affected 32 countries for 9
months, 8096 people became ill, of which 774 died (9% mortality). No
new cases of SARS-CoV have been report since 2004. 2. Another type of
coronavirus that caused SARS in humans, MERS-CoV, first detected in
Saudi Arabia in 2012. Ñonsidered bats can
be the natural reservoir of MERS-CoV, and single-humped camels can be
vectors. MERS-CoV has been founding in patients in 27 countries, mainly
in the Middle East, and remains endemic in many of them due to
circulation in camels. The last outbreak of MERS occurred in South
Korea in 2015, where the virus was imported from Kuwait (186 became ill
and 33 died – the elderly). As of November 2019, MERS-CoV caused 858
deaths out of 2494 laboratory-confirmed cases (33% mortality). 3.
SARS-CoV-2, like SARS-CoV and MERS-CoV, can cause severe respiratory
disease with a high mortality rate. Horseshoe bats considered the
natural reservoir of SARS-CoV2 (as well as SARS-CoV). There are two
versions for SARS-CoV2: the first is that is bat coronavirus and it has
spread from the seafood market, can crossed the interspecies barrier
and become pathogenic to humans; second, that during an experiment at
the Institute of Virology in Wuhan, the coronavirus accidentally struck
an employee in an aerosol, who became the "first patient". Discussion: The virion of coronaviruses is covered with a lipid shell
that clearly visible on electron microscopic images club-shaped spikes
length of 10 nm. Coronaviruses contain a positive single-stranded RNA
genome with a length of 26 to 32 thousand nucleotides (the largest
known RNA virus) and four structural proteins – spike (S), envelope
(E), membrane (M) and nucleocapsid (N). Protein S binds to target cell
receptors and triggers the infectious process, protein M plays a role
in shell formation and virion formation, and protein E forms pentameric
ion channels that destroy cell membranes during viral budding.
SARS-CoV-2 uses as receptors for cell penetration CD147 – glycoprotein
and angiotensin converting enzyme type 2 (angiotensin converting enzyme
2 – ACE2). CD147 expressed in the cell membrane of epithelial and
endothelial cells and T lymphocytes. Monoclonal antibodies against
CD147 prevent infection of SARS-CoV2 cells in vitro. Analysis of cell
samples for the presence of RNA sequences SARS-CoV-2 reveals
significant RNA transcription in the nasal epithelium and less in the
cells of the lower respiratory tract and alveolar epithelium. This
suggests that the upper rather than the lower respiratory tract is the
initial site of SARS-CoV-2 infection. ACE2 localized in type I and II
pneumocytes, vascular endothelial cells and enterocytes (therefore
symptoms from the respiratory tract are often accompanied by symptoms
from the gastrointestinal tract – nausea, diarrhea, etc.). SARS-CoV2
infection leads to cytopathic effects, including apoptosis, cell lysis,
and syncytium formation in lung tissue. After penetration into the host
cell, the SARS-CoV-2 genome attaches to the ribosomes, leading to the
translation of viral polyproteins, which subsequently processed by
viral proteolytic enzymes. As a result, of proteolysis the
protease-mediated virus 3CLpro (chymotrypsin-like protease) and PLpro
(papain-like protease), polyproteins are broken down into smaller
components, which plays a major role in mediating the replication and
transcription of viruses and promotes infection. Another RdRp
(RNA-dependent RNA-polymerase) enzyme, replicase, is important for
viral genome replication and products of new virions. Therefore, these
enzymes can considered as potential drug targets for the development of
therapeutic agents, as they are crucial for the survival, replication
and transmission of SARS-CoV-2. Therapeutic strategies is using for the
treatment of Covid-19 mainly divided into immune and antiviral. S, E,
M, N proteins, two replicase isoforms (RdRp1a and RdRp1ab) and
proteases (3CLpro and PLpro) can be considere as potential targets for
drugs and vaccines against SARS-CoV-2. Despite the many directions in the search the effective drugs for treatment Covid-19, today the most
effective strategy in therapy is access to oxygen and well-staffed
supportive care reduces mortality more than any medicinal product. Conclusion. The sudden appearance of SARS-CoV-2 and the pandemic
caused by this virus have posed a threat to the security of the global
health system. The scientific community around the world is working
hard to find effective drugs and vaccines against the new coronavirus.
In an effort to find the cure for Covid-19, WHO has developed the
SOLIDARITY concept, an international clinical trial as a common global
platform for improving scientific links and accelerating information
exchange. On the base of Mechnicov institute of microbiology and
immunology National academy of medical sciences of Ukraine also began
the study of the peculiarities of immunity Covid-19 for further
development the therapeutic and vaccine strategies.
Keywords:SARS-CoV-2; genome; pathogenesis; therapeutic strategies
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7-20
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Autoimmune rheumatic diseases and
autoinflammatory syndromes, facets of contact
Yeloyeva Z.V., Kiselyova L.P., Filonova T.O., Matvi³enko
S.O., Apanasenko O.N., Krivoshey A.V., Moskalenko A.S.
Introduction The peculiarity of the development of inflammation, its
acute and chronic course, various combinations of inflammatory
mediators, the nature and severity of the immune response is largely
arise from the genetic characteristics of the organism. It would be
logical to assume the key role of small mutations of genes in
initiating the development of autoaggression, activation of signaling
molecules, immunocompetent cells with violation of their cooperation,
production of pro-inflammatory cytokines, as a consequence of the
effect of persistent viral-bacterial infection and non-infectious
agents. In this regard, the comparative analysis of pathogenetic
mechanisms of development, clinical symptoms of autoimmune rheumatic
diseases and genetically determined autoinflammatory syndromes (AIDs),
the mutation of genes in the development of many of them has already
been proved, would be interesting. Material & methods The article is compiled based on the publications review. Results &
discussion At the heart of
the development of autoimmune rheumatic diseases lie mostly the changes
in the system of immunobiological surveillance, the adaptive link of
immunity. The elimination of T-lymphocyte anergy to autoantigens,
increased activity and expansion of clones of T and B lymphocytes, the
production of circulating autoantibodies upon activation of signaling
pathways / molecules, the secretion of proinflammatory cytokines
(IL-1,6, TNF-α) are the key links in immunopathogenesis of rheumatic
diseases The spectrum of autoimmune rheumatic diseases differs in
adults and children. Only children suffer from juvenile idiopathic
arthritis, juvenile dermatomyositis, neonatal lupus, Kawasaki disease,
and acute rheumatic fever. Both children and adults suffer from
systemic lupus erythematosus, systemic vasculitis, antiphospholipid
syndrome, and systemic scleroderma. Dominant symptoms in the debut of
autoimmune rheumatic diseases are prolonged fever, skin-vascular
syndrome, serositis, arthralgia, arthritis, myalgia, myositis, high
laboratory activity indicators. In contrast with most autoimmune disease
auto-inflammatory syndroms usually present during childhood. AIDs is a
heterogeneous group of rare, genetically determined states
characterized by unprovoked attacks of inflammation and manifested by
fever and clinical symptoms resembling rheumatic ones in the absence of
autoantibodies and / or antigen-specific T-lymphocytes. Diseases of
this group have both common and distinctive features that correspond to
the Eurofever register of more than 21 nosological forms, including
congenital recurrent febrile syndromes, cryopyrin-associated periodic
syndromes, granulomatous and pyogenic diseases, proteasome deficiency,
chronic recurrent multifocal osteomyelitis. Unlike of autoimmune diseases, the main role in the
initiation of autoinflammatory syndromes is played by mutations in
genes involved in the realization of the immune response of the innate
immunity system, as well as non-antigen-induced antibody synthesis or
activation of T and β-lymphocytes. A special place, as a trigger mechanism, in
the development of autologous means, belongs to the multiplate
cytoplasmic complex formed in neutrophils and macrophages - an
inflamasome regulating the secretion of pro-inflammatory cytokines and,
as a consequence, triggering an inflammatory response upon contact of
cells with infectious and non-infectious agents. The composition of the
inflamasome includes proteins encoded by genes, the specific mutations
of which cause the development of AIDs. Most clearly, the inflamasomes role in the pathogenesis
of AIDs can be traced to the example of Cryopyrin-associated Periodic
Syndromes (CARS), including Familial Cold Auto-inflammatory Syndrome
(FCAS), Muckle-Wells Syndrome (MWS) and Chronic Infantile Onset
Neurological Cutaneous Articular/ Neonatal Onset Multi-systemic
Inflammatory Disease - CINCA / NOMID syndrome. All three problems are
caused by a mutation of the CIASI gene located in the long arm of the
first pair of chromosomes and encoding the cryopyrin protein (NLRR3). Despite differences in pathogenesis and
distinctive features of a course of diseases, the targets of
inflammatory reaction in the compared groups of autoimmune rheumatic
diseases and autoinflammatory syndromes are identical: the skin,
joints, serous coverings, and the central nervous system. The
complication of both syndromes is the development of AA-amyloidosis,
and multi-organ failure. Conclusion Perhaps the disclosure, the understanding of more subtle
mechanisms of development, the cyclicity of the autoinflammatory
process, the spontaneous arrest of the inflammatory response,
unprovoked relapse attacks, as well as the determination of the
carriage of pathogenic mutations of genes responsible for the
development of AIDs in patients with autoimmune rheumatic diseases,
will help to identify that disturbed link in the general chain of
interconnection of cells of congenital and adaptive immunity, which is
a key factor in the development of autoaggression. So in order to
deepen our understanding of human innate immunity, and to offer more
targeted therapies for patients with autoimmune rheumatic diseases,
further studies on the genetics and molecular pathophysiology of
autoimmune rheumatic and autoinflammatory diseases are essential.
Keywords: Autoimmune rheumatic diseases, autoinflammatory
syndromes,facets of contact
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21-30
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Nuclear localization signal
peptides (NLS) and their role in viral pathogenicity
Nosalskaya Tatiana Nikolaevna, Martynov Artur Viktorovich,
Bomko Tatiana Vasilievna
The review provides data about nuclear localization signal
peptides (NLS) and their function in the cell, incl. with a viral
infection process. The binding, penetration, assembly, and budding of
viruses are currently being intensively studied in many systems.
However, the stages of nuclear transport during the penetration and
release of the virus have remained practically unexplored. NLS
were first identified in the large T antigen virus SV40 and from
nucleoplasmin, and then were identified in a large number of proteins.
They usually contain short base peptides includes lysine or arginine
residues in the form of mono- or bipartite signals. NLSs include the
"pat4" motif, which consists of a contiguous stretch four essential
amino acids (arginine and lysine). Both coronaviruses and arteriviruses
show similar genomic organization and belong to Nidovirales. Although
both families encode nucleoproteins (N-proteins), the main function of
which is to bind viral RNA, NLS-containing proteins has different sizes
and do not have significant homology. The review provides the
characteristics and structure of NLS for many viral proteins, and shows
their role in the pathogenicity of viruses.
Keywords: Nuclear localization signal, viruses, coronaviruses,
influenza, hepatitis, virulence
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31-40
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Some aspects
of studying the state of the oral microflora Dyachenko Valentina,
Voronkina Iryna, Maryuschenko Anatoly, Serdechna Eleonora,
Biryukova Svetlana, Kheder Said
The study of the microflora of the oral cavity plays an
important role in establishing the pathogenetic mechanisms of
purulent-inflammatory diseases of periodontal tissues. The human oral
microbiome includes more than 700 species of microorganisms that
inhabit various habitats, including teeth, gums, tongue, cheeks, hard
and soft palate, and tonsils. The term "microbiome" was coined in 2001
by Joshua Lederberg to denote the ecological set of commensal,
symbiotic and pathogenic microorganisms in our body that play the role
of "determinants of health and disease". Under the influence of various
factors in the qualitative and quantitative composition of the
microflora of the oral cavity there are changes. Her condition is
influenced by lifestyle, age, human nutrition, compliance with the
rules of oral hygiene, as well as the presence of various diseases,
including various disorders and diseases in the oral cavity, and other
factors. Because there is a constant flow of microorganisms that enter
the oral cavity from the environment, it is necessary to distinguish
them from endogenous species, which Theodore Rosebury called local
microbes or normal microflora. It is believed that the difference
between them cannot be established directly by studies of human
isolates alone. It is necessary to compare them with isolates from the
external environment to determine which clones are rapidly restored in
the host organism, or in the environment. Analysis of the oral
microbiome of healthy people using the latest advances in sequencing
technology revealed that most species of bacterial microorganisms in
healthy people are identical. Based on international research in recent
years, a database of phylogenetic data on the human oral microbiome -
Human Oral Microbiome Database (HOMD), which includes about 700 taxa
(individual species and subgroups) and 13 types of Actinobacteria,
Fusobacteria, Chlamidia, Chloroflexi, Furyarchaeota Proteobacteria
Spirochaetes, SR1, Synergistetes, Tenericutes and TM7. HOMD is the
first phylogenetic base of microbiome results, which aims to understand
the health and development of human oral diseases (including caries,
purulent-inflammatory periodontal diseases and others). In order to
determine the relative number of taxa and to identify new species of
oral microbiota, 36,043 clones of 16S rRNA genes with more than 1000
isolates were analyzed. The results revealed 1,179 taxa, of which about
280 species of bacteria were isolated culturally and received an
official name, about 8% - cultivated but not named, and 68% were
non-cultivated. Thus, it was confirmed that more than half of the
species of bacteria existing in the oral cavity cannot be cultured on
nutrient media. As for the classical bacteriological methods of
research, they are obviously used to the greatest extent in routine
practice, to study the state of the human microbiome, including the
oral cavity. The search for new methods of prevention and treatment of
purulent-inflammatory periodontal diseases is inextricably linked with
the isolation of pure cultures of pathogens and the study of their
biological properties: sensitivity to antibiotics, exposure to various
physical factors (ultraviolet, laser radiation, relativistic electrons,
etc.) cells.
Keywords:
oral microflora, studying, review
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41-44
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Experimental
works
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Immunological
features of umbilical cord blood of newborns born to women with
different implementation of intrauterine infection
Vygivska
L.A.
Introduction. Perinatal
infections (PNIs) have been increasingly referred to as one of the
causes of adverse effects of pregnancy recently, accompanied by an
inflammatory process, which is based on a highly integrated immune
response. Immunopathological reactions cause destabilization of the
whole homeostasis and create a favorable basis for the periodic
exacerbation of infectious and inflammatory diseases, and the inability
of the pregnant woman's body to adequate immune response is the cause
of the pathological course of pregnancy. Determination of immunological
parameters in the umbilical cord reflects the degree of formation of
the immune system of the fetus and thus characterizes the level of
anti-infective protection, which depends on the risk of infectious
abnormalities. Objective: To investigate the
immunological features of umbilical cord blood of newborns born to
women with different implementation of intrauterine infection (IUI). Materials and
methods. The study involved examination of 180 pregnant women
divided into 3 groups depending on the presence and nature of the
detected infection: Group I - viral (CMV, herpes simplex virus
types1,2,6), Group II - bacterial (chlamydia, ureaplasma, mycoplasma)
and Group III - mixed (viral and bacterial) infection. Each of these
groups was divided into 2 subgroups: with subsequent implementation of
the infection in newborns (1) and without it (0). This group was the
main one in the study. Control group included 50 patients with
physiological pregnancy. In clinical trials, samples of umbilical blood
obtained during childbirth from women with signs of IUI after the use
of different treatment regimens depending on the etiological factor of
PNI were studied. Results
and discussion.
Studies
have shown a significant decrease in the total number of CD3+T
lymphocytes, their subpopulations CD4+T-helpers,
CD8+T
suppressors, as well as CD25+
regulatory
T-cells in newborns with the implementation of IUI. The activity of
cytotoxic natural killer cells (CD16+NK),
which implement anti-infective protection, was higher in the main
groups than in the control, which is apparently due to the effect on
the fetus of a large number of antigens. The percentage of
B-lymphocytes in the umbilical blood of newborns with infectious
abnormalities was in the range of reference values, but in Group I-0 of
newborns the number of cells producing immunoglobulins was
significantly higher than in the same group with the implementation of
IUI, which may be associated with increased production of own
immunoglobulins due to insufficient transplacental transfer of IgG from
the mother. The increased concentration of IgM in the umbilical blood,
which was found in various clinical groups, indicates the activation of
humoral responses in the newborn in response to an infectious agent and
is a marker of IUI. In the groups with IUI implementation, the
concentration of IgM and IgG in the umbilical blood was lower by 20–30%
and 13–17%, respectively, than in similar comparison groups, which is
an important factor in reducing the anti-infective resistance of
newborns. The level of IgA in the clinical groups did not differ
statistically, although it exceeded the reference values. Conclusion. The
functional activity of umbilical blood phagocytes in groups with
clinical manifestations of IUI was characterized by a significant
decrease in the ability of neutrophilic umbilical blood granulocytes to
actively absorb and fully digest antigens. Such a defect in the
phagocytic protection of the fetus may be one of the probable factors
increasing the risk of infection.
Keywords:perinatal
infections; implementation of intrauterine infection; umbilical cord
blood; immunopathological reactions.
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45-49
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Determination of the presence of
specific antibodies against SARS-CoV-2 school-age children in Kharkiv
in September 2020
Volyansky A. Yu., Kuchma I. Yu., Davydova T. V., Kuchma
M.V., Yudin I.P.
Introduction. Recently, the world community and modern medical
science have faced the challenge of the emergence new human coronavirus
SARS-CoV-2 capable of causing severe acute respiratory syndrome. WHO
has named the disease caused by this virus Covid-19 (coronavirus
infection disease). SARS-CoV-2 was first detected in December 2019 in
Wuhan, from where it quickly spread to China and further around the
world. Due to the high contagiousness and pathogenicity of SARS-CoV-2,
WHO declared the global pandemic Covid-19 in early March 2020. The
features of the pathogenesis of Covid-19 determine the significant
frequency cases of this disease with a severe course. The severity
course of the disease and the consistency and completeness of the
immune response are interrelated: excessive and continued activation of
innate immunity factors with an increase in the cytokine profile is
observed in patients with severe course, and the transition to the
stage of production the specific antibodies is characterized by an
improvement in the patient's condition and recovery. The specific
humoral response is important in the formation of collective
post-infection and post-vaccination immunity and is an indicator of
overcoming epidemic processes in the population. The study is conducted
in the Mechnikov institute of microbiology and immunology National
Academy of Medical Sciences of Ukraine has one of the tasks of
determining specific antibodies to SARS-CoV-2 in different age groups
and monitoring the formation of the immune layer during the epidemic
process, as well as the duration of detection of specific
antibodies. Material & methods. A study was conducted to determine specific total
immunoglobulins, IgA, IgM and IgG to SARS-CoV-2 by enzyme-linked
immunosorbent assay (ELISA) among schoolchildren Kharkiv gymnasium ¹X
for 27-28 weeks of the pandemic (21-24/09/2020). The object of the
study were 439 samples of serum from children aged 6 to 16 years. Results & discussion. This phase of the study the presence of specific
SARS-CoV-2 antibodies in certain groups held at a time when the
territory of Ukraine still it was not observed a significant increase
in the number of patients with Covid-19 compared to Western Europe,
China, the USA and others, but with almost 6 months have passed since
the beginning of the pandemic. Most of the patients (98.4 %) in a
survey questionnaire and not noted the manifestations of acute
respiratory illness, excessive fatigue, temperature rise or other
manifestations characteristic of Covid-19. The study revealed total
SARS-CoV-2 antibodies in 20.3% of children, questionable levels total
antibodies were found in almost 9 %. IgA was detected (positive and
questionable) in 15.2 % of children, IgM – 7.7 %, and IgG – 14.3
%. In serum samples total SARS-CoV-2 antibody
positive/questionable in 23.6% of cases were identified only one of the
classes of immunoglobulins: IgA at 5,6 %, IgM – 3,3 %, IgG – 14,7 %. In
most samples two classes of antibodies were detected simultaneously –
67.4 % of samples and in 9.02% all three classes of immunoglobulins
were detected simultaneously. An interesting fact is predominantly
simultaneous presence in serum IgA and IgG in 60 samples (46.9%) with
total serum antibodies to SARS-CoV-2. Simultaneous detection IgM and
IgG was noted much less, only in 29 samples – 22.7%, the combination of
simultaneous presence IgA and IgM without presence IgG was detected in
one sample (0.78%). Conclusion. The data obtained indicate that more than 20% of
school-age children in the randomized sample had antibodies to
SARS-CoV-2 at 27-28 weeks of the pandemic (21-24/09/2020), despite the
fact that at the time of this study, our country has not yet faced an
avalanche of rising morbidity, which was observed in this period.
Covid-19 is known to be milder, asymptomatic or asymptomatic in
children, which is consistent with our lack of evidence in most
children for 6-8 weeks prior to the study, although it is possible to
assume that the infection occurred earlier. However, the simultaneous
presence in the serum SARS-CoV-2 IgA and IgG in 60 samples (46.9%) and
IgM and IgG in 29 samples – in 22.7%, which is approximately three
quarters of all children, which had positive antibodies to SARS-CoV-2,
according to traditional notions about the frequency of the formation
humoral response suggests a higher probability of infection during this
period to the coin determination of antibodies in blood serum. Obtained
in this study data provide an understanding certain features of the
formation SARS-CoV-2 post infectious immunity in school-age children
and delivered important issues that require further research.
Keywords: Covid-19 school-age children; antibodies to SARS-CoV-2
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50-55
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Identification of the Oxa-48 Gene
Coding For Carbapenem Resistance in Klebsiella pneumoniae and
Escherichia Coli at Dr. Muhammad Hoesin Central General Hospital,
Palembang
Muhammad Fitrizal
OXA-48 is a class D β-lactamase which is not inhibited by clavulanic acid, tazobactam and sulbactam, because their activity may be inhibited in vitro by
NaCl. Some b-lactamase enzymes hydrolyze carbapenem and are
therefore defined as hydrolysis of class D carbapenem b-lactamase. Carbapenem-resistant Klebsiella pneumoniae isolates were found in Istanbul, Turkey, in 2001. OXA-48
produces isolates that have the ability to fight drugs and cause very
beneficial resistance to β-lactams, including widely available cephalosporins, cephamycins, and monobactone and carbapenem. The OXA-48 gene is found on plasmids.
Antibiotic resistance is a phenomenon taken from the discovery of
antibiotics where there is a very good adaptability for bacteria which
are treated with various antibiotics. It has bacteria that have the
ability to mutate as a protection for survival. This study is a case
study involving the OXA gene as an encoder for carbapenem in Escherichia coli and Klebsiella pneumoniae using the Polymerase Chain Reaction (PCR) method to use
genotypes used in hospitals that can be used in Dr. RSUP. Mohammad
Hoesin Palembang. Bacterial isolates derived from patients with Klebsiella pneumoniae and Escherichia coli infections in the period September-November 2017 were
identified using Vitek 2 Compact. blaOXA-48 gene was detected by PCR, followed by visualization
through electrophoresis. The detection results were then analyzed by
comparing the pattern of antibiotic resistance. From 24 samples tested
only 1 (4.7%) positive samples were obtained from the bacterium Klebsiellapneumoniae and no positive gene was found in Escherichia coli. This study identified 1 (4.2%) samples that had a
positive OXA-48 gene while 23 (95.8%) samples had a neative OXA-48 gene.
Keywords: Klebsiella pneumoniae, Escherichia coli, OXA-48, Carbapenem
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56-62
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Theoretical
confirmation of prospectivity of application of metabolitic complexes
of lactobacilli and saccharomycetes in the fight antibiotic resistance of bacteria
Isayenko O. Yu., Babich Y. M., Gorbach T. V., Pivnenko S. Yu, Antusheva T. O.
Introduction. The global problem is the increase in the number of
infectious diseases caused by antibiotic–resistant pathogens. Measures
to control these microorganisms should be aimed at preventing the formation of antibiotic–resistant
populations of microorganisms and at inhibiting already established
resistant populations. The aim of the work is to substantiate the
perspective of using metabolic complexes of lactobacilli and
saccharomycetes to control antibiotic resistance of bacteria. Material & methods. Cellular structures of lactobacilli and saccharomycetes
(L / S) were received by irradiation with low–frequency ultrasonic
waves (generator G3–109) of suspensions of Lactobacillus rhamnosus GG (from symbiotic PREEMA, Schonen, Switzerland) and Saccharomyces boulardii (from probiotic drug BULARDI, Schonen, Switzerland). The
metabolites of L. rhamnosus GG (ML) and S. boulardii (MS) were received in their own cellular structures. The
combination of lactobacilli with saccharomycetes (MLS) and metabolites
of saccharomycetes (LS) in the cellular structures of lactobacilli.
Suspensions of microorganisms (resistant to antibiotics) gram–negative Pseudomonas aeruginosa PR, Acinetobacter baumannii PR, Klebsiella pneumoniae PR, Lelliottia amnigena (Enterobacter amnigenus) PR,
gram–positive
Staphylococcus aureus PR, Staphylococcus haemoliticus PR, Enterococcus faecalisPR, Corynebacterium xerosis PR, the cultures Corynebacterium spp. tox +, Streptococcus viridans, Streptococcus
pneumoniaå with an optical density of 5,0 units on the McFarland
scale (Densi–La–Meter (PLIVA–Lachema Diagnostika, (Czech Republic)) was
added to L / S / ML / MLS / MS / LS (experimental samples) or to 0.9%
sodium chloride solution (control samples) in a ratio of 1: 1. All
samples were incubated for 1 hour at a temperature of + 35 ± 1 ° C,
then the optical density was adjusted to 0.5 McFarland. Sowing was
carried out on Mueller–Hinton medium. After disks with antibiotics
(imipenem, vancomycin, cefotaxime, gentamicin, erythromycin,
ciprofloxacin, amicil, chloramphenicol, ampicillin, ceftazidime ,
ceftriaxone, tetracycline, levofloxacin, amoxiclav, azithromycin),
incubated (35 ± 1 ° C, 24 hours), measured the zones of growth
retardation of microorganisms around the discs with antibiotics. Results & discussion. Potentiation of antimicrobial activity in the combined
use of experimental samples with antibacterial drugs occurred in 88% of
combinations with ML, 83 % – with MLS, 85 % – with MS, 73 % – with LS.
Without increase in activity was substances with gentamicin, amicil,
ampicillin, ceftazidime were administered agains A. baumannii PR and with levofloxacin against S. aureus. Samples of ML over L (P = 0,005) and MLS over
L (P = 0,008) had the advantage of a general increase in the
diameters of the zones of growth inhibition of all tested pathogens.
These results indicate a statistically significantly greater inhibition
of growth of selected strains when combining antibiotics with metabolic
complexes than with cellular structures. Excellent enhancement was
observed when combining different antibacterial drugs with ML (on 5,5 ± 0,7, P <0,05), MLS (on 4,95 ± 0,6, P = 0,01) and MS (on 3,96±0,6, Ð=0,001) relative to control. More inhibition of growth was
observed of antibiotics with MLS than with MS (P = 0,02). A difference
between the efficacies of the metabolic complexes ML and MLS was not
found (P = 0,09). The presence of a large number of combinations of
metabolic complex – antibiotic with the ability to therapeutically
significant indicators to increase the antibacterial activity testifies
the effectiveness of the combined use of metabolites L. rhamnosus GG and S.boulardii with different drugs. Conclusion. Theoretically confirmed of perspectivity of application of metabolic complexes of
lactobacilli and saccharomycetes in the fight against antibiotic
resistance of bacteria. Synergistic combinations of lactobacilli and
saccharomycetes with antibiotics have been established. A
therapeutically significant increase in their combined antimicrobial
activity has been proven. This efficacy for different antibiotic–resistant strains
indicates the perspectives of using metabolic complexes of lactobacilli
and saccharomycetes to develop multifunctional antimicrobials
preparations with consequence the possibility of inhibiting antibiotic
resistance to already formed bacterial populations and at preventing
the formation of antibiotic–resistant populations of microorganisms.
Keywords: lactobacilli, saccharomycetes, multidrug–resistant
microorganisms, potentiation of antibiotics,
combinations metabolites with antibiotics, synergistic antibacterial
activitie
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63-69
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Activity of the COVID-19 pathogen
transmission mechanism and means of its interruption in modern
conditions
Zhalko-Titarenko V.P., Podavalenko À.P.
Introduction. During the 21st century, three coronavirus-related
pandemics have been reported. In 2019, a new virus called severe acute
coronavirus 2 syndrome (SARS-CoV-2) was confirmed. Currently, the
incidence of COVID-19 is growing daily and fatalities are registered in
many parts of the world, including Ukraine. The introduction of
quarantine somewhat halted the spread of the virus, but led to enormous
economic losses. Therefore, the introduction of personal protective
equipment could help break the chain of the COVID-19 epidemic process.
However, misconceptions about the transmission mechanism of SARS-CoV-2
and myths about personal protective equipment have led the population
of Ukraine, including medical workers, to ignore the routine wearing of
masks and respirators indoors and in transport, and to keep a distance
of 1.5 - 2 meters. Therefore, the purpose of the work was to study the
aerosol-aspiration mechanism of transmission of the SARS-CoV-2 virus
based on extrapolation from experimental studies and proof of the
effectiveness of personal protective equipment in interrupting the
transmission of the pathogen COVID-19. Material & metods. The results of an experimental study of the mechanism of
transmission of pathogens through the air in the laboratory of the
State Institution "Institute of Epidemiology and Infectious Diseases.
L.V. Gromashevsky National Academy of Medical Sciences of Ukraine ".
The motion of the aerosol cloud and its vorticity was recreated. The
formulas were used to determine the aerodynamic characteristics of the
process, in particular, time, distance, speed, acceleration and
deceleration, cutting size, aspiration flows and directions of aerosol
cloud movement. The risk of infection in a mobile atmosphere compared
to a stationary indoor atmosphere was calculated using a certain
formula, which included the number 3.14; the distance between a sick
(or carrier) and a healthy person; a factor of 0.4 that is equal to the
maximum diameter ("diameter") of the aerosol cloud. Results & discussion. The mechanism of transmission of the causative agent of
infectious diseases is realized through three stages - the isolation of
the pathogen from the body, its stay in the factors of transmission and
entry into a new organism. It was found that during expiratory acts,
the droplets do not scatter in a cone, but are released in separate
portions - aerosol clouds of irregular shape about 40 cm in diameter.
The movement of these clouds is characterized by a fading inertial run
up to 90 cm long, and having overcome this distance, they fall into
nearby air currents and, depending on the direction of the wind, can
move in different directions. In a closed room, on the way of its
movement, an aerosol cloud changes within 1 - 7 seconds. Super-large
droplets in the range of 500 - 1000 microns fly about 1 meter per
second along ballistic curves and fall to the floor, slightly smaller
droplets with a radius of 50 - 100 microns have time to dry out,
decrease in diameter and settle within the next few seconds. Droplets
in the 25 - 50 micron range settle in two and a half minutes, and even
smaller droplets can stay in the air for tens of minutes or even hours.
In confined spaces, the ventilation flows directed towards the exhaust
hood can prevent transmission of SARS-CoV-2 coronavirus. There is a
misconception that it is not possible to become infected with the
SARS-CoV-2 virus outdoors. The study showed that the risk of infection
in the open at a distance of 1 meter is 12%, at a distance of 2 and 4
meters, respectively, 6.3% and 3.3%. The rapid spread of the COVID-19
pandemic against the background of the susceptible population
contributed to the smoothing of annual seasonality, but its pronounced
manifestation in the long-term dynamics is not excluded. Large
dispersed droplets have been shown to settle mainly in the upper
respiratory tract and cause subclinical forms with symptoms of acute
respiratory viral infection and carrieris, while small ones can enter
the lower respiratory tract, causing severe acute pneumonia. The chain
infectious process is dose-dependent, and is largely determined by the
nature of the pathogen. The pattern of dose-dependence of the
infectious process is especially important for understanding the
epidemic and infectious processes in COVID-19. The two-week incubation
period suggests that the coronavirus population may grow rather slowly.
Therefore, it can be assumed that in the current COVID-19 epidemic
process, SARS-CoV-2 virus infection occurs at doses close to those that
cause the carrieris, ie relatively small doses. And the conversation is
a variant of the aerosol-aspiration mechanism of transmission of the
pathogen, which causes the transmission of small doses, and hence the
pandemic spread of asymptomatic and subclinical forms of coronavirus
infection. Therefore, in our opinion, the most adequate and effective
means of protection against COVID-19 is the routine use of masks and
respirators that meet the State Standard of Ukraine. Conclusion. The spread of COVID-19 occurs through aerosol clouds
mainly indoors (buildings, structures, transport), but the risk of
infection also remains on the street and in parks where there are
crowds. The severity of the disease depends on the loading of the
aerosol cloud with the SARS-CoV-2 virus, the dose and characteristics
of the aerosol droplets that enter the body. Large droplets, which get
into the body when talking, even in small doses, cause subclinical
forms and carriers, and fine - severe forms of COVID-19. Total mask
regimen interrupts the aerosol-aspiration mechanism of SARS-Cov-2
transmission by stopping its transmission from a COVID-19 patient to a
healthy person.
Keywords: COVID-19, pathogen transmission mechanism, interruption
in modern conditions
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70-75
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The effect of using whitening
creams that contain mercury in the community
Pipim Septiana Bayasari, Anis Irawan Anwar, Faridha Ilyas,
Anni Adriani
Background: Mercury is usually added to skin lightening products
because of its whitening effect. However, many cosmetics contain
mercury above 1000 ppm to enhance the whitening effect. Objectives: This research aimed to determine the effects of mercury
use in whitening creams for the general public. Methods: This research used descriptive-qualitative approach, the
method that used in this research is literature study which implemented
by recording the previous findings regarding to the variables of
conflict.. Results: Skin lightening products that contain
mercury are available for sale via the Internet, promoted online on
social media sites, and for sale via mobile applications. WHO states
that more than 90 creams from 15 countries have detectable mercury
concentrations below 1 ppm. Discussion:Thirty-four creams (10% of the sample) were found to have
high mercury levels, that is, above 1 ppm, in four of the 13 samples
from Indonesia, overall, mercury concentrations in this particular
product ranged from 93 ppm to over 16,000 ppm.Long-term adverse effects of using mercury cream on
health include kidney damage, skin rashes, skin discoloration, and
scarring, decreased skin resistance to bacterial and fungal infections,
anxiety, and depression.
Keywords: Health Effects, Cosmetics, Mercury, Whitening Cream, Skin.
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76-82
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Case Report
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Cataract Extraction on One Seeing
Eye in Patient with Behcet’s Disease
Nurul Fitri Shabrina, Dicky Hermawan, Randi Montana
Introduction : Behcet’s Disease is autoimmune relapsing systemic
vasculitis of unknown that can cause uveitis and affect the anterior
and posterior segment of the eye. Cataract is the most common
complication of anterior segment caused by recurrent uveitis. This case
was to report the outcomes and complications of cataract surgery in
patients with Behçet's disease. Case report: A of 35 years old man complained progressive visual
loss since 6 months before admission with VA 1 meter finger counting
and NLP. There was history of recurrent redness on both eyes
accompanied by blurred vision and pain, oral aphtosis, and genital
aphtosis since three years before admission but didn’t seek help
because of low access to healthcare in his previous home. From anterior
segment examination there was posterior synechia, cloudy lens, pupil
secclusion, rubeosis iridis, flare and cell suggesting chronic uveitis.
There was elevated IOP of 43.4 mmHg on left eye. Retinal vasculitis was
found on posterior segment examination. Result: After combination of methylprednisolone and azathioprine
for one year, there were improvement of quieter condition on both
eyes and decreased left eye IOP of 37.2 mmHg. Lens extraction with iris
retractor was done after three months of no inflammatory response.
Despite uneventfull surgery and combination of immunosupresive
medication, the visual acuity are not much improved and there was optic
atrophy from the complication of chronic Behcet’s disease.
Conclusion: In patient with chronic Behcet’s disease, ocular
involvement can affect both anterior and posterior segments with poor
visual outcome regarding its complication
Keywords :Behcet’s disease, Cataract extraction, one-seeing eye
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83-87
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